Zeytin (Olea europaea) Yaprağı Ekstraktının Model Organizma Galleria mellonella Hemosit Aracılı Bağışıklık Tepkileri Üzerine Etkileri

Zeytin ağacı (Olea europaea) ülkemizin de içerisinde yer aldığı Akdeniz bölgesinde yaygın olarak yetiştirilen bir türdür. Bu ağacın meyvesi ve yağından yararlanıldığı kadar, yapraklarından da halk hekimliğinde yararlanılmaktadır. Antioksidan, anti-enflamatuar ve antibiotik etkilerinin yanında kalp hastalıklarından korunma, karaciğer yağlanması, diabet gibi bazı yaygın hastalıklara karşı da etkili olduğu bilinmektedir. Zeytin yaprağının en önemli bileşeni oleuropeindir. Çalışmamızda; insan patojenleri için sıklıkla kullanılan model organizma Galleria mellonella (Büyük Balmumu Güvesi) kullanıldı. Zeytin yaprağı ekstraktı ile muamele edilen G. mellonella larvalarında toplam hemosit sayısı, enkapsülasyon ve melanizasyon bağışıklık tepkileri incelendi. Toplam hemosit sayısının tespiti ve enkapsülasyonmelanizasyon tepkilerinin belirlenmesi için faz-kontrast mikroskobu kullanıldı. Bulgularımıza göre 1000 ppm dozunda zeytin yaprağı ekstraktı bağışıklığı desteklemektedir.

Effects of Olive (Olea europaea) Leaf Extract on Hemocyte-Mediated Immune Responses of The Model Organism Galleria mellonella

Olive tree (Olea europaea) is a species widely grown in the Mediterranean region, where our country is located. As well as the fruit and oil of this tree, its leaves are used in folk medicine. In addition to its antioxidant, anti-inflammatory and antibiotic effects, it is known to be effective against some common diseases such as protection from heart disease, fatty liver and diabetes. The most important component of olive leaf is oleuropein. In our study; the model organism Galleria mellonella (Greater Wax Moth), which is frequently used for human pathogens has been used. The effects of olive leaf extract treated to G. mellonella larvae on total hemocyte count, encapsulation and melanization immune responses were investigated. A phase-contrast microscope was used to determine the total hemocyte count and the encapsulation-melanization responses. According to our findings, 1000 ppm dose of olive leaf extract supports immunity.

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Altincicek, B., Linder, M., Linder, D., Preissner, K.T. ve Vilcinskas, A. 2007. Microbial metalloproteinases mediate sensing of invading pathogens and activate innate immune responses in the lepidopteran model host Galleria mellonella. Infection and Immunity, 75(1): 175-183.
Altuntaş, H., Kılıç, A.Y., Uçkan, F. ve Ergin, E. 2012. Effects of gibberellic acid on hemocytes of Galleria mellonella L.(Lepidoptera: Pyralidae). Environmental Entomology, 41(3): 688- 696.
Antolovich, M., Prenzler, P., Robards, K. ve Ryan, D. 2000. Sample Preparation in the Determination of Phenolic Compounds in Fruits. Analyst, 125: 989- 1009.
Bronskill, J. 1961. A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). Journal of Lepidopterists Society, 15(2): 102-104.
Champion, O.L., Cooper, I.A.M., James, S.L., Ford, D., Karlyshev, A., Wren, B.W., Duffield, M., Oyston, P.C.F. ve Titball, R.W. 2009. Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis. Microbiology, 155: 1516-1522.
Cook, S.M. ve McArthur J.D. 2013. Developing Galleria mellonella as a model host for human pathogens. Virulence, 4: 350- 353.
Cutuli, M. A., Petronio, G., Vergalito, F., Magnifico, I., Pietrangelo, L., Venditti, N. ve Di Marco, R. 2019. Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing. Virulence, 10(1): 527-541.
De Filippo, J. ve Beck, G. 2018. Cytokines of Invertebrate Immunity. “Alınmıştır: Reference Module in Life Sciences, ELSEIVER, ISBN:978-0-12-809633-8.
Duman, E. ve Altuntaş, H. 2018. Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). Biological Diversity and Conservation, 773-0918.
Eguchi, D. ve Iwabuchi K. 2006. A new cell line from the wax moth Galleria mellonella Linne (Lepidoptera: Pyralididae). In Vitro Cellular & Developmental BiologyAnimal, 42(1-2): 1-3.
El, S.N. ve Karakaya, S. 2009. Olive tree (Olea europaea) leaves: potential beneficial effects on human health. Nutrition Reviews, 67(11): 632-638.
Er, A. ve Keskin, M. 2015. Influence of abscisic acid on the biology and hemocytes of the model insect Galleria mellonella (Lepidoptera: Pyralidae). Annals of the Entomological Society of America, 109(2): 244-251.
Er, A., Taşkıran, D. ve Sak, O. 2017. Azadirachtin-induced effects on various life history traits and cellular immune reactions of Galleria mellonella (Lepidoptera: Pyralidae). Archives of Biological Sciences, 69(2): 335-344.
Er, A., Uçkan, F., Rivers, D.B., Ergin, E. ve Sak, O. 2010. Effects of parasitization and envenomation by the endoparasitic wasp Pimpla turionellae (Hymenoptera: Ichneumonidae) on hemocyte numbers, morphology, and viability of its host Galleria mellonella (Lepidoptera: Pyralidae). Annals of the Entomological Society of America, 103(2): 273-282.
Furneri, P.M., Marino, A., Saija, A., Uccella, N. ve Bisignano, G. 2002. In vitro antimycoplasmal activity of oleuropein. International Journal of Antimicrobial Agents, 20: 293-296.
Geyikoğlu, F., Emir, M., Çolak, S., Koç, K., Türkez, H., Bakır, M. ve Özek, N.S. 2017. Effect of oleuropein against chemotherapy drug-induced histological changes, oxidative stress, and DNA damages in rat kidney injury. Journal of Food and Drug Analysis, 25(2): 447-459.
Giamarellos-Bourboulis, E.J., Geladopoulos, T., Chrisofos, M., Koutoukas, P., Vassiliadis, J., Alexandrou, I., Tsaganos, T., Sabracos, L., Karagianni, V., Pelekanou, E.,Tzepi, I., Kranidioti, H., Koussoulas, V. ve Giamarellou, H. 2006. Oleuropein: a novel immunomodulator conferring prolonged survival in experimental sepsis by Pseudomonas aeruginosa. Shock, 26(4):410-416.
Gwokyalya, R. ve Altuntaş, H. 2019. Boric acidinduced immunotoxicity and genotoxicity in model insect Galleria mellonella L.(Lepidoptera: Pyralidae). Archives of Insect Biochemistry and Physiology, 101: e21588.
Ignasiak, K. ve Maxwell, A. 2017. Galleria mellonella (greater wax moth) larvae as a model for antibiotic susceptibility testing and acute toxicity trials. BMC Res Notes,10: 428-436.
Jiravanichpaisal P., Lee B. ve Soderhall K. 2006. Cell-mediated immunity in arthropods: Hematopoiesis, coagulation, melanization and opsonization, Immunobiology, 211: 213–236.
Junqueira, J.C. 2012. Galleria mellonella as a model host for human pathogens: Recent studies and new perspectives. Virulence, 3:6, 474–476.
Kaya, S. 2015. Konağa Verilen İndol-3-Asetik Asitin ve Kaliks Sıvısının Konak Galleria mellonella L. (Lepidoptera: Pyralidae) ve Parazitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) Hemositlerine Etkisi, Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Ana Bilim Dalı, Doktora Tezi, Çanakkale, s. 98.
Krautz, R., Arefin, B. ve Theopold, U. 2014. Damage signals in the insect immune response. Frontiers in Plant Science, 5: 342.
Kurt, D. ve Kayış, T. 2015. Effects of the pyrethroid insecticide deltamethrin on the hemocytes of Galleria mellonella. Turkish Journal of Zoology, 39(3): 452- 457.
Lee, M.J. ve Anstee, J.H. 1995. Phenoloxidase and its zymogen from the haemolymph of larvae of the lepidopteran Spodoptera littoralis (Lepidoptera: Noctuidae). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 110(2): 379-384.
Loof, T.G., Schmidt, O., Herwald, H. ve Theopold, U. 2011. Coagulation Systems of Invertebrates and Vertebrates and Their Roles in Innate Immunity: The Same Side of Two Coins? Innate Immunity, 3: 34-40.
Magrone, T., Spagnoletta, A., Salvatore, R., Magrone, M., Dentamaro, F., Russo, M.A. ve Jirillo, E. 2018. Olive leaf extracts act as modulators of the human immune response. Endocrine, Metabolic & Immune Disorders-Drug Targets, 18(1): 85-93.
Martinez-Martos, J.M., Mayas, M.D., Carrera, P., de Saavedra, J.M.A., Sánchez-Agesta, R., Arrazola, M. ve Ramírez-Expósito, M.J. 2014. Phenolic compounds oleuropein and hydroxytyrosol exert differential effects on glioma development via antioxidant defense systems. Journal of Functional Foods, 11: 221-234.
Mukherjee, K., Altincicek, B., Hain, T., Domann, E., Vilcinskas, A. ve Chakraborty, T. 2010. Galleria mellonella as a Model System for Studying Listeria Pathogenesis. Applied and Environmental Microbiology, 76(1): 310-317.
Mwakalukwa, R., Ashour, A., Amen, Y., Niwa, Y., Tamrakar, S., Miyamoto, T. ve Shimizu, K. 2019. Anti-allergic activity of polyphenolic compounds isolated from olive mill wastes. Journal of Functional Foods, 58: 207-217.
Nappi, A.J. ve Christensen, B.M. 2005. Melanogenesis and associated cytotoxic reactions: applications to insect innate immunity. Insect Biochemistry and Molecular Biology, 35(5): 443-459.
Omar, S.H. 2010. Oleuropein in olive and its pharmacological effects. Scientia Pharmaceutica, 78(2): 133-154.
Park, J.H., Jung, J.H., Yang, J.Y. ve Kim, H.S. 2013. Olive leaf down-regulates the oxidative stress and immune dysregulation in streptozotocin-induced diabetic mice. Nutrition Research, 33(11): 942-951.
Persia, F.A., Mariani, M.L., Fogal, T.H. ve Penissi, A.B. 2014. Hydroxytyrosol and oleuropein of olive oil inhibit mast cell degranulation induced by immune and nonimmune pathways. Phytomedicine, 21(11): 1400-1405
Sak, O., Uçkan, F. ve Ergin E. 2006. Effects of cypermethrin on total body weight, glycogen, ptotein and lipid contents of Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae). Belgian Journal of Zoology, 136: 53-58
Servili, M. ve Montedoro G.F. 2002. Contribution of phenolic compounds to virgin olive oil quality. European Journal of Lipid Science and Technology, 104: 602-613.
Sezer, B. ve Özalp, P. 2015. Effects of pyriproxyfen on hemocyte count and morphology of Galleria mellonella. Fresenius Environmental Bulletin, 24(2): 621-625.
Sheehan, G., Garvey, A., Croke, M. ve Kavanagh, K. 2018. Innate humoral immune defences in mammals and insects: The same, with differences? Virulence, 9(1): 1625-1639.
Sigle, L.T. ve Hillyer, J.F. 2018. Mosquito Hemocytes Associate With Circulatory Structures That Support Intracardiac Retrograde Hemolymph Flow. Frontier in Physiology, 9: 1187.
Silva, S., Gomes, L., Leitao, F., Coelho, A.V. ve Boas, L.V. 2006. Phenolic compounds and antioxidant activity of Olea europaea L. fruits and leaves. Food Science and Technology International, 12(5): 385-395.
Sirianni, R., Chimento, A., De Luca, A., Casaburi, I., Rizza, P., Onofrio, A. ve Pezzi, V. 2010. Oleuropein and hydroxytyrosol inhibit MCF-7 breast cancer cell proliferation interfering with ERK1/2 activation. Molecular Nutrition & Food Research, 54(6): 833-840.
Tassetto, M., Kunitomi, M. ve Andino R. 2017. Circulating immune cells mediate a systemic RNAi based adaptive antiviral response in Drosophila. Cell, 169(2): 314–325.
Uçkan, F., Er, A., ve Ergin, E. 2010. Levels of encapsulation and melanization in Galleria mellonella (Lepidoptera: Pyralidae) parasitized and envenomated by Pimpla turionellae (Hymenoptera: Ichneumonidae). Journal of Applied Entomology, 134: 718-726.
Vilmos, P. ve Kurucz É. 1998. Insect immunity: evolutionary roots of the mammalian innate immune system. Immunology Letters, 62: 59–66.
Wu, G., Xu, L. ve Yi, Y. 2016. Galleria mellonella larvae are capable of sensing the extent of priming agent and mounting proportionatal cellular and humoral immune responses. Immunology Letters, 174: 45-52.
Yao, J., Wu, J., Yang, X., Yang, J., Zhang, Y. ve Du, L. 2014. Oleuropein induced apoptosis in HeLa cells via a mitochondrial apoptotic cascade associated with activation of the c-Jun NH2-terminal kinase. Journal of Pharmacological Sciences, 125(3): 300- 311.
Yateem, H., Afaneh, I. ve Al-Rimawi, F. 2014. Optimum conditions for oleuropein extraction from olive leaves. International Journal of Appiled Science and Technology, 4(5): 153-157
Yin, M., Jiang, N., Guo, L., Ni, Z., Al-Brakati, A.Y., Othman, M.S. ve Kassab, R.B. 2019. Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats. Life Sciences, 232: 116634.
Yücel, M.S. ve Kayış, T. 2018. Imidacloprid induced alterations in oxidative stress, biochemical, genotoxic and immunotoxic biomarkers in nonmammalian model organism Galleria mellonella L. (Lepidoptera: Pyralidae). Journal of Environmental Science and Health, Part B, 1–8.
Zhang, J.Y., Yang, Z., Fang, K., Shi, Z.L., Ren, D.H. ve Sun, J. 2017. Oleuropein prevents the development of experimental autoimmune myocarditis in rats. International Immunopharmacology, 48: 187-195.