Muammer KIRICI, Ünal İSPİR, Muhammed ATAMANALP

Efficacy of a Formalin-Killed Vaccine against Flavobacterium psychrophilum in Rainbow Trout (Oncorhynchus mykiss)

Gökkuşağı alabalığı yavru sendromu için aşı geliştirilmesi tam hücre bakterinlerine dayalı olarak yapılmaktadır. Bu çalışmada, Flavobacterium psychrophilum’a karşı sonraki çalışmalarda aşı geliştirilmesinde kullanılacak aday suşun koruyucu etkileri araştırıldı. 30, 60 ve 90 dakika için 1×108 CFU/ml formalinle inaktive edilmiş hücrelere maruz kalan gökkuşağı alabalıklarının nisbi hayatta kalma oranlarındaki (RPS) değişimler belirlendi. RPS oranları sırasıyla %21.74, %34.78 ve %43.48 olarak kaydedildi. Sonuç olarak, bu suşun zayıf bir koruyucu etkiye sahip olduğu ve aşı yapımı için kullanımının uygun olmadığı kanaatine varıldı.

Anahtar Kelimeler:

Oncorhynchus mykiss, Flavobacterium psychrophilum, Gökkuşağı alabalığı yavru sendromu, nisbi hayatta kalma oranları, aşı.

Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nda Flavobacterium psychrophilum’a Karşı Formalinle Öldürülmüş Bir Aşının Etkisi

Vaccine development for rainbow trout fry syndrome (RTFS) has been based primarily on whole-cell bacterins. In this study, protective effects of candidate strain to develop a vaccine in future studies against to Flavobacterium psychrophilum were investigated. Alteration in the relative percentage of survival rate (RPS) of rainbow trout exposed to 1×108 CFU/mL formalin-killed cells for 30, 60 and 90 min. Upon challenge with a strain the RPS of respectively, 21.74%, 34.78% and 43.48% was recorded. The results suggested that a weak protection had obtained with this strain, therefore; the strain was not suitable for use in vaccine development.

Keywords:

Oncorhynchus mykiss, Flavobacterium psychrophilum, rainbow trout fry syndrome, relative survival rates, vaccine.,

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