The Use of Syrian Sumac (Rhus coriaria) as a Meat Tenderizer: Effect on Fat, Protein and Collagen Profiles on Pectoralis superficialis Cut

The Syrian Sumac (Rhus coriaria) is a widely used spice in the Arab world of attractive economic importance in food, cosmetic and pharmaceutical industries. Meat tenderness is one of the very most important factors for customers’ acceptability. The global objective of this study was to add an additional value to Sumac by evaluating its meat tenderizing effect. Crude sumac fruits were used to create three different extracts (aqueous, ethanolic and purified enzymatic extract). Pectoralis superficialis cuts were treated with the extracts and studied for their shear stress, pH, protein and fat contents and collagen. The hypothesis that Sumac could have a meat tenderizing effect was supported by our results showing a significant decrease in shear stress and protein content with increase in collagen solubility. Moreover, an effect on decreasing meat fat was detected, where the aqueous sumac extract decreased significantly the fat percentage in meat. The active enzymes in Sumac were shown to be variate in nature, lipase and protease, with a significant effect on collagen, thus proving Sumac’s possibility to be potentially used as a meat tenderizer

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Abdelmalek A. 2013. The Effect of Different Spices on increasing Poultry Meat Shelf Life. Assiut Environmental Research Journal. Volume 38.

Abed OO, Arkan MA, Kholoud AJ. 2013. The Antibacterial Effect of Sumac and Myrtle Extracts on the Bacteria Causative of Soft Rot Disease in Potatoes. Babilonia University Journal. Volume 21.

AbouReidah I, Jamous R, Shtayeh M. 2014. Phytochemistry, pharmacological properties and industrial applications of Rhus coriaria L. JJBS. Volume 7. Pages 233-244. Palestine.

AlJaber G. 2008. Antibacterial effect of two phenolic extracts from Rhus coriaria. BRJ. Volume 22: 22-32. Iraq.

Almouwaly K, Alflayeh K, Ali A. 2013. Antioxidant and free radical scavenging effects of Iraqi sumac Rhus coriaria L. BSJ. Volume 10: 921-933. Iraq.

Anaee R, Abdullah H, Tareq M. 2016. Sumac extract as green inhibitor for steel in seawater. IJSRSET. Volume 2: 353-358. Iraq.

Anweri T, Sharma M, Khan G, Iqbal M, Ali M, Alam M, Safhi M, Gupta M. 2013. Rhus coriaria ameliorates insulin resistance in non-insulin-dependent diabetes mellitus (NIDDM) rats. APPDR. Volume 70: 861-867. Saudi Arabia.

Araújo I, Bezerra T, Nascimento E, Gadelha C, Santi-Gadelha T, Madruga M. 2018. Optimal conditions for obtaining collagen from chicken feet and its characterization. JFST. ISSN 1678- 457X. DOI: https://doi.org/10.1590/fst.27517. Brazil.

Ardalani H, Moghadam M, Hadipanah A, Fotovat F, Azizi A, Soltani J. 2016. Identification and characterization of chemical composition of Rhus coriaria L. fruit from Hamadan, Western Iran. Journal of Herbal Drug. Vol. 6, No. 4: 195-198. Iran.

Arshad M, Kwon J, Imran M, Sohaib M, Aslam A, Nawaz I, Amjad Z, Khan U, Javed M. 2016. Plant and bacterial proteases: A key towards improving meat tenderization, a mini review. Cogent Food and Agriculture. Volume 2: 1261780. DOI: http://dx.doi.org/10.1080/23311932.2016.1261780. Pakistan.

Arunmozhivarman K, Abraham R, Rao A, Parthiban M. 2017. Extraction and Molecular Characterization of Collagen from Poultry meat Processing by-Product (Chicken Skin). Int. J. Pure App. Biosci. Volume 5 (5): 1085-1091. DOI: http://dx.doi.org/10.18782/2320-7051.5337

Asgarpanah J, Saati S. 2013. An overview on phytochemical and pharmacological properties of Rhus coriaria L. RJP. Volume 1: 47-54. Iran.

Bekhit A, Hopkins D, Geesink G, Franks P. 2014. Exogenous proteases for meat tenderization. CRFSN. Volume 54: Pages 1012- 1031. DOI: https://doi.org/10.1080/10408398.2011.623247

Bellew J, Brooks J, McKenna D, Savell J. 2002. Warner–Bratzler shear evaluations of 40 bovine muscles. MSJ. Volume 64: 507-512. DOI: 10.1016/S0309-1740(02)00242-5. USA.

Bergman I. and Loxley R. 1963. Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. JAC. Volume 35:1961.

Bursal E, Köksal E. 2011. Evaluation of reducing power and radical scavenging activities of water and ethanol extracts from sumac Rhus coriaria. FRI. Volume 44: 2217-2221. DOI: 10.1016/j.foodres.2010.11.001. Turkey.

Calkins C, Sullivan G. 2007. Adding Enzymes to Improve Meat Tenderness. R&KM National Cattlemen’s Beef Association. www.beefresearch.org.

Chaiwut P, Pintathoga P, Rawdkuen S. 2010. Extraction and three-phase partitioning behavior of proteases from papaya peels. JoPB. Volume 45: 1172-1175. Thailand.

Christensen M, Torngren M, Gunvig A, Rozlosnik N, Lametsch R, Karlsson A, Ertbjerg P. 2009. Injection of marinade with actinidin increases tenderness of porcine M. biceps femoris and affects myofibrils and connective tissue. JoSFA. Abstract.

Codex Alimentarius. 1999. Recommended Methods of Analysis and Sampling CXS 234-1999. International Food Standards.

Cross HR, Carpenter ZL, Smith GC. 1973. Effects of intramuscular collagen and elastin on bovine muscle, tenderness. Journal of Food Science. Volume 38: 998.

Dabas D. 2016. Polyphenols as Colorants. Adv Food Technol Nutr Sci Open J. Volume 2: S1-S6. DOI: 10.17140/ AFTNSOJ-SE-2-101USA.

Dinh T. 2006. Meat quality: understanding of meat tenderness and influence of fat content on meat flavor. Science & Technology Development. Volume 9: 65-70.

Dogan A. 2015. Potential therapeutic properties of Rhus coriaria on streptozotocin-induced diabetic rats. Yuzuncu YIL University. Volume 54: 2092-2102. DOI: https://doi.org/10.3109/13880209.2016.1145702. Turkey.

Duman Y, Kaya E. 2013. Three-Phase Partitioning as a Rapid and Easy Method for the Purification and Recovery of Catalase from Sweet Potato Tubers (Solanum tuberosum). Appl Biochem Biotechnol. Volume 170: 1119–1126. DOI 10.1007/s12010-013-0260-9

Ertürk O. 2010. Antibacterial and Antifungal Effects of Alcoholic Extracts of 41 Medicinal Plants growing in Turkey. Czech J. Food Sci. Vol. 28 No. 1: 53–60. Turkey.

Font-i-Furnols M, Candek-Potokar M, Maltin C, Povse M. 2015. A handbook of reference methods for meat quality assessment. European Cooperation in Science and Technology.

Food and Agriculture Organization of the United Nations, FAO. 1986. Manuals of Food Quality Control; Food Analysis: General Techniques, Additives, Contaminants and Composition. FAO Food and Nutrition Paper. Rome.

Gagaoua M, Hafid K, Hoggas N. 2016. Data in support of three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes. Elsevier Data in Brief. Volume 6: 634–639. DOI: https://doi.org/10.1016 /j.dib.2016.01.014. Algeria.

Gagaoua M, Hoggas N, Hafid K. 2014. Three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes. Int. J. Biol. Macromol. Volume 73: 245– 252. DOI: http://dx.doi.org/10.1016/j.ijbiomac.2014.10.069 014. Algeria.

Handa S, Khanuja S, Longo G, Rakesh D. 2008. Extraction Technologies for Medicinal and Aromatic Plants. International Centre for Science and High Technology. Italy, Earth, Environmental and Marine Sciences and Technologies. Hanna Instruments. pH measurement SOP.

Hill F. 1966. The solubility of intramuscular collagen in meat animals of various ages. Journal of Food Science. Volume 31:161.

Hosseinzadeh J, Feyzollahzadeh M, Afkari A. 2013. The physical and chemical properties of kiwifruit harvested at four stages. Bulg. J. of Agri. Sci. Volume 19: 174-180. Iran.

Institute of Agriculture and Natural Resources. Bovine Myology. 2017. University of Nebraska. USA. (https://bovine.unl.edu/main /index.php?lang=English&musID=86&muscle=null&what= muscleDescriptions&listBy=commonName)

Jin Y, Fu W, Ma M. 2011. Preparation and structure characterization of soluble bone collagen peptide chelating calcium. African Journal of Biotechnology. Volume 10: 10204-10211. DOI: 10.5897/AJB10.1923. China.

King N, Whyte R. 2006. Does it look cooked? A Review of Factors that Influence Cooked Meat Color. JoFS. Volume 71: R31-R40. DOI: 10.1111/j.1750-3841.2006.00029.

Kossah R, Nsabimana C, Zhang H, Chen W. 2010. Optimization of extraction of polyphenols from Syrian Sumac (Rhus coriaria) and Chinese Sumac (Rhus typhina) Fruits. RJoPhytochem. Volume 4: 146-153.

Kossah R, Nsabimana C, Zhao J, Chen H, Tian F, Zhang H, Chen W. 2009. Comparative Study on the Chemical Composition of Syrian Sumac (Rhus coriaria L.) and Chinese Sumac (Rhus typhina L.) Fruits. Pak. J. Nutr. Volume 8: 1570-1574.

Lewis D. 1988. Application of actinidin from kiwifruit to meat tenderization and characterization of beef muscle protein hydrolysis. Journal of Food Biochemistry. Volume 12: 147- 158. DOI: 10.1111/j.1745-4514.1988.tb00368.x USA.

Listrat A, Lebret B, Louveau I, Astruc T, Bonnet M, Lefaucheur L, Picard B, Bugeon J. 2016. How Muscle Structure and Composition Influence Meat and Flesh Quality. The Scientific World Journal.

Lovrien R, Dennison C. 1997. Three Phase Partitioning: Concentration and Purification of Proteins. Book: Protein Expression and Purification. Volume 11: 149–165. New York.

Lucas J, Castaneda D, Hormigo D. 2017. New trends for a classical enzyme: Papain, a biotechnological success story in the food industry. Trends in Food Science and Technology Elsevier. DOI: 10.1016/j.tifs.2017.08.017. Spain.

Meat Technology Information Sheet. 1998. Crude Fat Determination – Soxhlet Method. Food and Science Australia.

Mitchell H, Zimmerman R, Hamilton T. 1926. The Determination of the Amount of Connective Tissue in Meat. Department of Animal Husbandry, University of Illinois. http://www.jbc.org/

Mohammadi S, Kouhsari M, Feshani M. 2010. Antidiabetic properties of the ethanolic extract of Rhus coriaria fruits in rats. DARU J. of Pharm. Sci. Volume 18: 270-275. Iran.

Morgan J, Savell B, Hale D, Miller R, Shackelford S. 1991. Traits of "Tender" and "Tough" meat. J. Anim. Sci. Volume 69: 3274-3283.

Nagappan R. 2012. Evaluation of aqueous and ethanol extract of bioactive medicinal plant, Cassia didymobotrya (Fresenius) Irwin & Barneby against immature stages of filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae). Asian Pacific Journal of Tropical Biomedicine. Volume 9: 707-711. DOI: 10.1016/S2221-1691(12)60214-7. Ethiopia.

Narsaiah K, Shyam N, Suresh K, Borah D, Desh B, Singh S. 2010. Tenderizing effect of blade tenderizer and pomegranate fruit products in goat meat. J Food Sci Technol Volume 48: 61–68. DOI 10.1007/s13197-010-0127-9. Italy

Ozcan M, Haciseferogullari H. 2004. A condiment sumac fruits: some physicochemical properties. Bulg. J. of Plant Physiol. Volume 30: 74-84. Turkey.

Rachana C, Jose V. 2014. Three Phase Partitioning - A Novel Protein Purification Method. National Institute of Animal Nutrition and Physiology. Volume 6: 3467-3472. India.

Raodah M, Alia Z, Feleeha H. 2014. The antioxidant and Antimicrobial activity of Syrian Sumac (Rhus coriaria) Fruits Extract. J. of Nat. Sci. R. Volume 4: 36-40. Iraq.

Recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology on the Nomenclature and Classification of Enzymes by the Reactions they Catalyze. http://www.sbcs.qmul.ac.uk /iubmb/enzyme/

Runs K, Wui D, Pritchard R. Steps for Warner-Bratzler Shear Force Assessment of Cooked Beef Longissimus Steaks at South Dakota State University. Department of Animal and Range Sciences. USA.

Schmid F. 2001. Biological Macromolecules: UV-Visible Spectrophotometry. Encyclopedia of Life Sciences. DOI: https://doi.org/10.1038/npg.els.0003142

Seggern V, Drew D, Calkins, Chris R. 2005. Physical and Chemical Properties of 39 Muscles from the Beef Chuck and Round. Nebraska Beef Cattle Reports. 323. http://digitalcommons.unl.edu/animalscinbcr/323

Sepos E. 2012. Standard Operating Procedure Rotary Evaporator in the P.O.W.E.R. Laboratory.

Shabbir A. 2012. Rhus coriaria Linn, a Plant of Medicinal, Nutritional and Industrial Importance: A Review. Journal of Animal and Plant Sciences. Volume 22: 505-512. Pakistan.

Shackelford S, Wheeler T, Koohmaraie M. 1999. Evaluation of Slice Shear Force as an Objective Method of Assessing Beef Longissimus Tenderness. American Society of Animal. Volume 10: 2693-2699. DOI: 10.2527/1999.77102693x

Sharmila S, Rebecca J, Das M, Saduzzaman M. 2012. Isolation and partial purification of Protease from plant leaves. Journal of Chemical and Pharmaceutical Research. Volume 6: 1153- 1156. India.

Shidfar F, Hosseinic S. 2014. The Effect of Sumac Rhuscoriaria L. Powder on Serum Glycemic Status, ApoB, ApoA-I and Total Antioxidant Capacity in Type 2 Diabetic Patients. Iranian Journal of Pharmaceutical Research. Volume 13: 1249-1255.

Sugiyama S, Hirota A, Okada C, Yorita T, Sato K, Ohtsuki K. 2005. Effect of Kiwifruit Juice on Beef Collagen. J Nutr Sci Vitaminol. Volume 51: 27-33. Tokyo, Japan.

VELP Scientifica. Nitrogen and Protein Determination in Meat according to the Kjeldahl method. Reference: AOAC 981.10 Crude Protein in Meat.

Wada M, Suzuki T, Yaguti Y, Hasegawa T. 2002. Effects of pressure treatments with kiwi fruit protease on adult cattle semitendinosus muscle. Journal of Food Chemistry. Volume 78: 167-171.

Wall K, Kerth C, Miller R, Alvarado C. 2017. Grilling temperature effects on tenderness, juiciness, flavor and volatile aroma compounds of aged ribeye, strip loin, and top sirloin steaks. MSc Thesis. Texas A&M University.

Whitehurst R, Oort M. 2010. Enzymes in Food Technology. 2nd Edition. Chapter 12.

Wilson M, Felker J. 2011. Better Crops with Plant Food. International Plant Nutrition Institute. Vol. 95. Page 27. Canada.

Yu T, Morton J, Clerens S, Dyer J. 2016. Cooking-Induced Protein Modifications in Meat. Comprehensive Reviews in Food Science and Food Safety. Volume 16: 141-159. DOI: 10.1111/1541-4337.12243

Zhu X. 2017. Actinidin Treatment and Sous Vide Cooking: Effects on Tenderness and In Vitro Protein Digestibility of Beef Brisket. MSc Master Thesis. Massey University. New Zealand.