Semra Küçük, Sema Midilli, Mehmet Güler, Deniz Çoban

Akut ve Kronik Kadmiyum Konsantrasyonlarına Maruz Bırakılmış Sarı Prenses Balıklarının Histolojik Değişiklikleri

Bu çalışmada, histolojik değişimleri incelemek için sarı prenses balıkları (Labidochromis caeruleus) akut cadmiyum konsantrasyonlarına (0, 50, 60, 70, 80 mg l-1 ) 72 saat süreyle ve kronik kadmiyum konsantrasyonlarına (0,00; 0,25; 0,50; 1,00; 2,00 mg l-1 ) 20 gün süreyle maruz bırakılmıştır. Ayrıca kronik deneme sonunda arta kalan balıklar 10 gün süreyle kadmiyum içermeyen temiz suya maruz bırakılmıştır. Bu iki denemeden sonra seçilmiş balıklardan solungaç, karaciğer ve dalak örnekleri toplanmıştır. Doku örnekleri standart hematosilen-eozin tekniğiyle hazırlanmıştır. Kadmiyum artan konsantrasyonlara bağlı olarak balıklarda önemli bozukluklara neden olmuştur. Akut maruziyet sonrası solungaçlarda ödem, hiperplazi, epitel dokuda bozukluklar; karaciğerde vakuoleşme ve nekroz; dalakta hemosterin kümeciklerinde artış ve nekroz gibi çeşitli bozukluklar saptanmıştır. Kronik maruziyet sonrasında solungaçlarda ödem, anörizm, sekonder lamellerde dejenerasyon; karaciğerde dejenerasyon ve nekroz; dalakta dejenerasyon ve granuloma gibi bazı semptomlar bulunmuştur. Ayrıca kronik kadmiyum maruziyetinden sonraki iyileşme evresinde, solungaçlarda ödem, hiperplazi ve anörizm; karaciğerde vakular dejenerasyon ve nekroz; dalakta granüloma ve dejenersyon gibi bazı benzer semptomlar gözlenmiştir.

Histological Changes of Electric Yellow Cichlid (Labidochromis caeruleus) Exposed to Acute and Chronic Cadmium Concentrations

In this study, electric yellow cichlid were (Labidochromis caeruleus) exposed to acute cadmium(Cd) concentrations (0, 50, 60, 70, 80 mg l-1) for 72 h, and chronic Cd concentrations (0.00, 0.25,0.50, 1.00, 2.00 mg l-1) for 20 d to examine histological alternation. In addition to the recovery wasdetected after 10 d. After chronic trial, rest of the fish were exposed to just water not containingcadmium for 10 day for recovery. Gills, liver, muscle, and spleen samples were collected fromrandomly selected fish. The tissue samples prepared with standard techniques for haematoxylin andeosin (H&E). Cadmium depending on concentrations increase caused severe disorders on fish. Afteracute exposure, several changes were detected such as edema, hyperplasia, breakdown in epithelialtissues of gills; vacuolation and necrosis in liver; increase of hemosterin clustering and necrosis inspleen. After chronic exposure, some symptoms were also found such as edema, aneurysm,degermation of secondary lamellae in gills; degeneration and necrosis in liver; degeneration andgranulomas in spleen. Additionally, after recovery of chronic Cd exposure some of the samesymptoms were observed such as edema, hyperplasia and aneurysm in gills; vacuolar degenerationand necrosis in liver; granulomas and degeneration in spleen

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