Gulten Okmen, Mahabbat Mammadhkanli, Kutbettin Arslan

The Antibacterial Activities of Lavandula angustifolia L., Mentha piperita L., and Ribes nigrum L. against Oral Bacteria, and Their Antioxidant Activities

There is an expanding interest in medicinal and aromatic plants as a natural alternative to synthetic drugs, especially to antimicrobial agents due to the growing problem of antibiotic resistance. In recent years, a lot of reports have been published on the antimicrobial activity of the plant extracts. This study was used Lavandula angustifolia L., Mentha piperita L., and Ribes nigrum L. as plant materials. This study aims to test the plant extracts against oral bacteria. Its purpose is to produce directly comparable, quantitative, antimicrobial data, and in addition to containing very little information of the different extracts against oral pathogens. Disc diffusion method was studied for antimicrobial activity tests. Also, minimum inhibitory concentration (MIC) values were determined in this study. Additionally, the extracts were tested against stable 2,2-diphenyl-1-picryl-hydrazyl- hydrate• (DPPH•) free radicals for non-enzymatic antioxidant activity. This study was used Trolox (6-hydroxy-2,5,7,8-tetra-methyl chroman-2-carboxylic acid) as standard. The extracts showed different inhibition zones against bacteria. The methanol extract of Lavandula showed the highest inhibition zone against the oral pathogen MBKK5. The positive control was penicillin (10 μg). The lowest MIC value was taken at 6500 μg /ml concentration of the plant extracts. The highest DPPH• radical scavenging activity was found in Ribes nigrum extract as 36%. As a result, plant extracts have antibacterial and antioxidant potentia

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Akgül H, Mohammed FS, Kına E, Uysal İ, Sevindik M, Doğan M. 2022. Total antioxidant and oxidant status and DPPH free radical activity of Euphorbia eriophora. Turkish Journal of Agriculture- Food Science and Technology, 10(2): 272-275. https://doi.org/10.24925/turjaf.v10i2.272-275.4685
Aytaç Z, İğci BK. 2012. Bitki Sistematiği (Plant Systematics, Simpson’dan çeviri). Ankara: Nobel Yayıncılık. ISBN 978- 605-133-350-2.
Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45: 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
Bayrak D, Okmen G, Arslan A. 2017. The biological activities of Lavandula stoechas L. against food pathogens. International Journal of Secondary Metabolite, 4(3): 270-279. https://doi.org/10.21448/ijsm.372221
Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel- Wissenschaft und-Technologie, 28: 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Cavanagh HM, Hipwell M, Wilkinson JM. 2003. Antibacterial activity of berry fruits used for culinary purposes. Journal of Medicinal Food, 6(1): 57-61. https://doi.org/10.1089/10966 2003765184750
Chitme HR, Chandra R, Kaushik S. 2003. Studies on antidiarrheal activity of Calotropis gigantea R. Br. in experimental animals. Journal of Pharmacy and Pharmaceutical Sciences, 7: 70-75. PMID: 15144737
Chung JY, Choo JH, Lee MH, Hwang JK. 2006. Anticariogenic activity of macelignan isolated from Myristica fragrans (nutmeg) against Streptococcus mutans. Phytomedicine, 13(4): 261-266. https://doi.org/10.1016/j.phymed.2004. 04.007
CLSI, 2003. Clinical and Laboratory Standards Institute. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. Approved Standard M7-A 6th edn. National Committee for Clinical Laboratory Standards. Wayne: Philadelphia.
CLSI, 2006. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 16th Informational Supplement M100-S16. National Committee for Clinical Laboratory Standards. Wayne: Philadelphia.
Çinbilgel İ, Kurt Y. 2019. Research on species diversity and ethno botanical utilization of Lamiaceae family in southern Türkiye. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 23(1): 90-107. https://doi.org/10.19113/sdufenbed.449607
Danh LT, Han LN, Triet NDA, Zhao J. 2013. Comparison of chemical composition, antioxidant and antimicrobial activity of lavender (Lavandula angustifolia L.) essential oils extracted by supercritical CO2, hexane and hydro distillation. Food and Bioprocess Technology, 6: 3481-3489. https://doi.10.1007/s11947-012-1026-z
Davis PH. 1965. Flora of Türkiye and the East Aegean Islands. Volume I. 568 pp. Edinburgh: Edinburgh University Press. eISBN 978-1-4744-6605-9.
Davis PH, Mill RR, Tan K. 1988. Flora of Türkiye and the East Aegean Islands. Volume X. 590 pp. Edinburgh: Edinburgh University Press. ISBN 9780852245590.
Davis PH. 1978. Flora of Türkiye and the East Aegean Islands. Volume VI. 827 pp. Edinburg: Edinburgh at the University Press. ISBN 0-85224-336-7.
Ergün N, Okmen G, Erdal P, Cantekin Z, Ergün Y. 2018. The antibacterial activities of Lavandula stoechas and Crepis sancta leaf and flower against mastitis pathogens and enzymatic and non- enzymatic antioxidant activities of the extracts. Turkish Journal of Agriculture- Food Science and Technology, 6(5): 543-549. https://doi.org/10.24925/ turjaf.v6i5.543-549.1692
Güner A, Aslan S, Ekim T, Vural M, Babaç MT. 2012. Türkiye Bitkileri Listesi (Damarlı Bitkiler). 1290 sayfa. İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. ISBN 978-605-60425-7-7.
Horvath P, Koscova J. 2017. In vitro antibacterial activity of Mentha essential oils against Staphylococcus aureus. Folia Veterinaria, 61(3): 71-77. https://doi.org/10.1515/fv-2017-0030
Houston MC. 2005. Nutraceutical, vitamins, antioxidants and minerals in the prevention and treatment of hypertension. Progress in Cardiovascular Diseases, 47: 396-449. https://doi.org/10.1016/j.pcad.2005.01.004
IHME, 2018. Institute for Health Metrics and Evaluation. Findings from the Global Burden of Disease Study 2017. Available from: https://www.healthdata.org/sites/ default/files/files/policy_report/2019/GBD_2017_Booklet_I ssuu_2.pdf [Accessed 01 February 2022].
İlkimen H, Gülbandılar A. 2018. Lavanta, ada çayı, kekik ve papatya ekstrelerinin antimikrobiyal etkilerinin araştırılması. Türk Mikrobiyoloji Cemiyeti Dergisi, 48(4): 241-246. doi:10.5222/TMCD.2018.241
Kendir G, Köroğlu A, Özek G, Özek T, Başer KHC. 2019. Glandular trichome structures and chemical composition of the volatiles of five Ribes species from Türkiye. Journal of Essential Oil Research, 31(2): 111-119. https://doi.org/10. 1080/10412905.2018.1547226
Kendir G, Köroğlu A, Özkan S, Özgacar SÖ, Karaoglu T, Gargari S. 2016. Evaluation of antiviral and antimicrobial activities of Ribes species growing in Türkiye. Journal of Biologically Active Products from Nature, 6(2): 136-149. https://doi.org/10.1080/22311866.2016.1202141
Korkmaz N, Dayangaç A, Sevindik M. 2021. Antioxidant, antimicrobial and antiproliferative activities of Galium aparine. Journal of Faculty of Pharmacy of Ankara University, 45(3): 554-564.
Krisch J, Galgóczy L, Papp T, Vágvölgyi C. 2014. Antimicrobial and antioxidant potential of waste products remaining after juice pressing. Annals of The Faculty of Engineering Hunedoara – Journal of Engineering, 4: 1584-2665.
Ludwiczuk A, Kiełtyka-Dadasiewicz A, Sawicki R, Golus J, Ginalska G. 2016. Essential oils of some Mentha species and cultivars, their chemistry and bacteriostatic activity. Natural Product Communications, 11(7): 1015-1018. https://doi.org/10.1177/1934578X1601100736
Mak KK, Day JR. 2011. Dental health behaviours among early adolescents in Hong Kong. Int. Journal of Dental Hygiene, 9(2): 122-126. https://doi.org/10.1111/j.1601-5037.2010. 00452.x
Okmen AS, Okmen G, Arslan A, Vurkun M. 2017. Antibacterial activities of Mentha piperita L. extracts against bacteria isolated from soccer player’s shoes and its antioxidant activities. Indian Journal of Pharmaceutical Education and Research, 51(3): 163-169. doi:10.5530/ijper.51.3s.5
Petersen PE. 2003. The World Oral Health Report 2003: Continuous improvement of oral health in the 21st century- the approach of the WHO Global Oral Health Programme. Community Dentistry and Oral Epidemiology, 31(1): 3-24. doi: 10.1046/j.2003.com122.x
Pramila DM, Xavier R, Marimuthu K, Kathiresan S, Khoo ML, Senthilkumar M, Sathya K and Sreeramanan S. 2012. Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae). Journal of Medicinal Plants Research, 6(2): 331- 335. doi: 10.5897/JMPR11.1232
Priya AM, Manikandan M, Kalaiselvi G, Arun P, Chinnaswamy P, Selvam K. 2007. Screening of antibacterial activity of Mentha piperita L. Asian Journal of Microbiology Biotechnology and Environmental Sciences, 9(4): 1049- 1052.
Prusinowska R, Smigielski K, Stobiecka A, Kunicka-Styczynska A. 2016. Hydrolates from lavender (Lavandula angustifolia) – their chemical composition as well as aromatic, antimicrobial and antioxidant properties. Natural Product Research, 30(4): 386-393. doi: 10.1080/14786419.2015. 1016939
Raghavan R, Devi MPS, Varghese M, Joseph A, Madhavan SS, Sreedevi PV. 2018. Effectiveness of Mentha piperita leaf extracts against oral pathogens: An in vitro study. The Journal of Contemporary Dental Practice, 19(9): 1042-1046. doi: 10.5005/jp-journals-10024-2378
Rapper S, Viljoen A, and Vuuren S. 2016. The In vitro antimicrobial effects of Lavandula angustifolia essential oil in combination with conventional antimicrobial agents. Evidence-Based Complementary and Alternative Medicine, Volume 2016: Article ID 2752739, 9 pages. https://doi.org/10.1155/2016/2752739
Rasooli I, Gachkar L, Yadegarinia D, Rezaei MB, Astaneh SDA. 2008. Antibacterial and antioxidant characterization of essential oils from Mentha piperita and Mentha spicata grown in Iran. Acta Alimentaria, 37(1): 41-52. https://doi.org/10.1556/aalim.2007.0019
Rodrigues F, Lehmann M, Amaral VSD, Reguly ML, Andrade HHRD. 2007. Genotoxicity of three mouthwash products, cepacol, periogard, and plax, in the Drosophila wing-spot test. Environmental and Molecular Mutagenesis, 48(8): 644- 649. https://doi.org/10.1002/em.20332
Saeed S, Tariq P. 2005. Antibacterial activities of Mentha piperita, Pisum sativum and Momordica charantia. Pakistan Journal of Botany, 37(4): 997-1001.
Schmidt E, Bail S, Buchbauer G, Stoilova I, Atanasova T, Stoyanova A, Krastanov A, Jirovetz L. 2009. Chemical composition, olfactory evaluation and antioxidant effects of essential oil from Mentha x piperita. Natural Product Communications, 4(8): 1107-1112. http://dx.doi.org/10. 1177/1934578X0900400819
Seçmen Ö, Gemici Y, Görk G, Bekat L, Leblebici E. 1998. Tohumlu Bitkiler Sistematiği. 396 sayfa. İzmir: Ege Üniversitesi Fen Fakültesi Kitaplar Serisi. ISBN 9754830282.
Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresenius Environmental Bulletin, 26(7): 4757-4763.
Singh R, Shushni MA, Belkheir A. 2015. Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry, 8(3): 322-328. https://doi.org/10.1016/j.arabjc. 2011.01.019
Smigielski K, Raj A, Krosowiak K, Gruska R. 2009. Chemical composition of the essential oil of Lavandula angustifolia cultivated in Poland. Journal of Essential Oil-Bearing Plants, 12(3): 338-347. https://doi.org/10.1080/0972060X.2009.106 43729
Sokovic M, Glamoclija J, Marin PD, et al. 2010. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules, 15: 7532-7546. https://doi.org/10.3390/molecules15117532
Thosar N, Basak S, Bahadure RN, Rajurkar M. 2013. Antimicrobial efficacy of five essential oils against oral pathogens: An in vitro study. European Journal of Dentistry, 7: 71-77. https://doi.org/10.4103/1305-7456.119078
Uğur A, Everest A. 2017. Mersin ilinde antidepresan ilaç ve benzeri bitki satış oranları. Bağımlılık Dergisi, 18(4): 152-157.
Uysal İ, Mohammed FS, Şabik AE, Kına E, Sevindik M. 2021. Antioxidant and oxidant status of medicinal plant Echium italicum collected from different regions. Turkish Journal of Agriculture-Food Science and Technology, 9(10): 1902- 1904. https://doi.org/10.24925/turjaf.v9i10.1902-1904.4588
Üstü Y, Uğurlu M. 2019. Lavantanın Tıbbi Kullanımı. Ankara Medical Journal, 19(2): 416-418. https://doi.org/10.17098/ amj.575563
Vagiri M, Ekholm A, Andersson SC, Johansson E, Rumpunen K. 2012. An optimized method for analysis of phenolic compounds in buds, leaves, and fruits of black currant (Ribes nigrum L.). Journal of Agricultural and Food Chemistry, 60(42): 10501-10510. https://doi.org/10.1021/jf303398z
WHO, 2012. World Health Organization. Available from: http://www.who.int/mediacentre/factsheets/fs318/fr/# [Accessed 22 September 2019]