Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes

The Myrtle plant (Myrtus communis L.) is a plant species of the Myrtaceae family and a member of the maquis community, which naturally spreads in Mediterranean regions. Being resistant to arid conditions, the ecological distribution areas of the myrtle plant have been allowed to expand. The myrtle plant has been used of medicinal and aromatic plants, having interesting and beautiful flowers, and rich nutrient content of the fruit in terms of valuable phytochemicals, in particular, the nutritional content of its fruits and valuable metabolites have allowed the myrtle plant to be among the healthier foods. Antioxidant activity, which neutralizes reactive oxygen species (ROS), which causes many medical problems, is one of the most important features of the myrtle plant. Investigation of the biosynthesis of anthocyanin, which leads to antioxidant activity, and determination of the biosynthesis in different tissues and genotypes is important, especially in the development of production activities. Furthermore, this study aimed to investigate the anthocyanin biosynthesis in different genotypes with white and black fruits and various tissues of genotypes. For this purpose, the expression levels of CHS, CHI, F3H and PAL genes, which are involved in the anthocyanin biosynthesis pathway, were determined by qRT-PCR. In the study, it was determined that there was an increase in the level of genes related to the biosynthesis of anthocyanin in the leaf and fruit tissues of the genotype with white fruits. It was determined that the expression level of genes related to the biosynthesis of anthocyanin was observed to be higher in the leaf and fruit tissues of the genotype with black fruits, and the highest gene expression level was found in black fruits. It was observed that anthocyanin biosynthesis was synthesized in different tissues of the plant, and anthocyanin biosynthesis was higher in fruits compared to leaves.

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Aka Kaçar Y, Biçen B, Şimşek Ö, Dönmez D, Erol M. 2020. Evaluation and comparison of new type of temporary immersion system (TIS) bioreactors for myrtle (Myrtus communis L.). Applied Ecology and Environmental Research, 18(1): 1611-1620.
Ban T, Ishimaru M, Kobayashi S, Goto-Yamamoto N, Horiuchi S. 2003. Abscisic acid and 2, 4-dichlorophenoxyacetic acid affect the expression of anthocyanin biosynthetic pathway genes in ‘Kyoho’grape berries. The Journal of Horticultural Science and Biotechnology, 78(4): 586-589.
Ben-Simhon Z, Judeinstein S, Trainin T, Harel-Beja R, Bar- Ya’akov I, Borochov-Neori H, Holland D. 2015. A" White" anthocyanin-less pomegranate (Punica granatum L.) caused by an insertion in the coding region of the Leucoanthocyanidin Dioxygenase (LDOX; ANS) gene. PLoS One, 10(11): e0142777.
Boss PK, Davies C, Robinson SP. 1996a. Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L. cv Shiraz grape berries and the implications for pathway regulation. Plant physiology, 111(4): 1059-1066.
Boss PK, Davies C, Robinson SP. 1996b. Expression of anthocyanin biosynthesis pathway genes in red and white grapes. Plant molecular biology, 32(3): 565-569.
Castellarin SD, Di Gaspero G. 2007. Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines. BMC Plant Biology, 7(1): 46.
Castellarin SD, Pfeiffer A, Sivilotti P, Degan M, Peterlunger E, Di Gaspero G. 2007. Transcriptional regulation of anthocyanin biosynthesis in ripening fruits of grapevine under seasonal water deficit. Plant, Cell and Environment, 30(11): 1381-1399.
Chusak C, Pasukamonset P, Chantarasinlapin P, Adisakwattana S. 2020. Postprandial glycemia, insulinemia, and antioxidant status in healthy subjects after ingestion of bread made from anthocyanin-rich riceberry rice. Nutrients, 12(3): 782.
Durbin ML, McCaig B, Clegg MT. 2000. Molecular evolution of the chalcone synthase multigene family in the morning glory genome. Plant molecular evolution, 79-92.
Feng S, Wang Y, Yang S, Xu Y, Chen X. 2010. Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10. Planta, 232(1): 245-255.
Fulcrand H, Dos Santos PJC, Sarni-Manchado P, Cheynier V, Favre-Bonvin J. 1996. Structure of new anthocyanin-derived wine pigments. Journal of the Chemical Society. Perkin Transactions 1(7): 735-739.
Guo J, Wang MH. 2010. Ultraviolet A-specific induction of anthocyanin biosynthesis and PAL expression in tomato (Solanum lycopersicum L.). Plant Growth Regulation, 62(1): 1-8.
Han M, Yang C, Zhou J, Zhu J, Meng J, Shen T, Xin Z, Li H. 2020. Analysis of flavonoids and anthocyanin biosynthesis- related genes expression reveals the mechanism of petal color fading of Malus hupehensis (Rosaceae). Brazilian Journal of Botany, 43(1): 81-89.
Harel-Beja R, Tian L, Freilich S, Habashi R, Borochov-Neori H, Lahav T, Trainin T, Doron-Faigenboim A, Ophir R, Bar- Ya’akov I. 2019. Gene expression and metabolite profiling analyses of developing pomegranate fruit peel reveal interactions between anthocyanin and punicalagin production. Tree Genetics and Genomes, 15(2): 1-13.
Hasegawa H, Fukasawa-Akada T, Okuno T, Niizeki M, Suzuki M. 2001. Anthocyanin accumulation and related gene expression in Japanese parsley (Oenanthe stolonifera, DC.) induced by low temperature. Journal of Plant Physiology, 158(1): 71-78.
Hennia A, Miguel MG, Nemmiche S. 2018. Antioxidant activity of Myrtus communis L. and Myrtus nivellei Batt. and Trab. extracts: A brief review. Medicines, 5(3): 89.
Janićijević J, Tošić S, Mitrović T. 2007. Flavonoids in plants. Proceeding of the 9th Symposium on Flora of Southeastern Serbia and Neighbouring Regions. Niš, Serbia, 153-156.
Medda S, Sanchez-Ballesta MT, Romero I, Dessena L, Mulas M. 2021. Expression of Structural Flavonoid Biosynthesis Genes in Dark-Blue and White Myrtle Berries (Myrtus communis L.). Plants, 10(2): 316.
Niu SS, Xu CJ, Zhang WS, Zhang B, Li X, Lin-Wang K, Ferguson IB, Allan AC, Chen KS. 2010. Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor. Planta, 231(4): 887 899. https://doi.org/10.1007/s00425-009- 1095-z.
Pahlke G, Ahlberg K, Oertel A, Janson-Schaffer T, Grabher S, Mock HP, Matros A, Marko D. 2021. Antioxidant Effects of Elderberry Anthocyanins in Human Colon Carcinoma Cells: A Study on Structure–Activity Relationships. Molecular nutrition and food research, 65(17): 2100229.
Palapol Y, Ketsa S, Lin-Wang K, Ferguson IB, Allan AC. 2009. A MYB transcription factor regulates anthocyanin biosynthesis in mangosteen (Garcinia mangostana L.) fruit during ripening. Planta, 229(6): 1323-1334. https://doi.org/10.1007/s00425-009-0917- 3.
Rouholamin S, Zahedi B, Nazarian-Firouzabadi F, Saei A. 2015. Expression analysis of anthocyanin biosynthesis key regulatory genes involved in pomegranate (Punica granatum L.). Scientia Horticulturae, 186: 84-88.
Sezgin AC. 2014. Meyve, sebze ve sağlımız (Fruit, vegetable and our health). About Thıs Journal, 46: 46.
Speer H, D’Cunha NM, Alexopoulos NI, McKune AJ, Naumovski N. 2020. Anthocyanins and human health—A focus on oxidative stress, inflammation and disease. Antioxidants, 9(5): 366.
Şimşek Ö, Biçen B, Dönmez D, Kaçar YA. 2017. Effects of different media on micropropagation and rooting of Myrtle (Myrtus communis L.) in in vitro conditions. International Journal of Environmental and Agriculture Research, 3(10).
Tena N, Martín J, Asuero AG. 2020. State of the art of anthocyanins: Antioxidant activity, sources, bioavailability, and therapeutic effect in human health. Antioxidants, 9(5): 451.
Ünal AN, Açar E, Şimşek Ö, Kaçar YA. 2021. Determınatıon Of Expressıon Levels Of Genes Related To Anthocyanın Synthesıs In Some Pomegranate Genotypes. International Journal of Agricultural and Natural Sciences, 14(3), 255-263.
Wrolstad RE, Durst RW, Lee J. 2005. Tracking color and pigment changes in anthocyanin products. Trends in Food Science and Technology, 16(9): 423-428.
Xie R, Zheng L, He S, Zheng Y, Yi S, Deng L. 2011. Anthocyanin biosynthesis in fruit tree crops: Genes and their regulation. African Journal of Biotechnology, 10(86): 19890- 19897.
Yamuangmorn S, Prom-u-Thai C. 2021. The Potential of High- Anthocyanin Purple Rice as a Functional Ingredient in Human Health. Antioxidants, 10(6): 833.
Yan F, Li H, Zhao P. 2019. Genome-Wide Identification and transcriptional expression of the PAL Gene family in common Walnut (Juglans regia L.). Genes, 10(1): 46.
Yu XZ, Fan WJ, Lin YJ, Zhang FF, Gupta DK. 2018. Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis. Ecotoxicology, 27(3): 325-335.
Zaidi SHR, Zakari SA, Zhao Q, Khan AR, Shah JM, Cheng F. 2019. Anthocyanin accumulation in black kernel mutant rice and its contribution to ROS detoxification in response to high temperature at the filling stage. Antioxidants, 8(11): 510.