Raphanus Sativus Yumrusu Belirli Ekstraktlarının Etkisinin Vicia Faba Üzerinde Botrytis Cinerea’ya Karşı Toplam Protein İçeriği ve Peroksidaz Aktivitesinin Değerlendirilmesi

Tıbbi bitki Raphanus sativus L. yumru kökünün su, etanol ve metanol ekstraktlarının Vicia faba L. (bakla) yapraklarında Botrytis cinerea fungusunun oluşturacağı kurşuni küf hastalığına karşı toplam protein içeriği ve peroksidaz (POX) aktivitesi bakımından bitkide meydana getirebileceği antioksidan savunma sistemi spektrofotometrik olarak analiz edilmiştir. R. sativus yumru kökünün su, etanol ve metanol ekstraktlarının stok solüsyonları dimetil sülfoksit (DMSO) ile hazırlanmıştır. 105 spor/mL B. cinerea spor süspansiyonu hazırlanmıştır. V. faba yapraklarına sadece distile su (negatif kontrol), sadece DMSO, sadece B. cinerea (pozitif kontrol), sadece ekstrakt uygulamaları (distile su, etanol ve metanol ekstraktı) ve ekstrakt uygulamaları sonrasında B. cinerea uygulaması gerçekleştirilmiştir. Yapraklar, analizi yapılacak olan yöntemlere uygun olarak ekstrakte edilmiştir. Ekstraktlardan elde edilen süpernatantlardan analizler spektrofotometrik olarak gerçekleştirilmiştir. Araştırma sonucunda, toplam protein içeriğinin distile su uygulanan (negatif kontrol) gruba kıyasla ekstrakt:fungus uygulama gruplarında artış gösterdiği saptanmıştır. Fakat sadece fungus uygulanan (pozitif kontrol) gruba kıyasla genel olarak azaldığı belirlenmiştir. POX aktivitesinin ise, negatif kontrol grubuna kıyasla artış gösterdiği saptanmıştır. Toplam protein içeriği ve POX aktivitesi, sadece ekstrakt uygulanan gruplarda negatif kontrol grubuna göre artış göstermiştir. En yüksek POX aktivitesi, 10 mg/mL distile su:fungus uygulamasından elde edilmiştir. Bu açıdan, ekstrakt:fungus uygulanan gruplar arasında en iyi antioksidan aktivitenin 10 mg/mL distile su:fungus uygulaması olduğu belirtilebilmektedir. Bu açıdan bakıldığında, R. sativus saf su, etanol ve metanol ekstraktlarının V. faba yapraklarında antioksidan savunma yanıtı verdiği belirtilebilmektedir.

Evaluation of the Effects of Specific Extracts of Raphanus Sativus Tuber on Total Protein Content and Peroxidase Activity Against Botrytis cinerea in Vicia Faba

The antioxidant defense system of the medicinal plant Raphanus sativus L. tuber in terms of total protein content and peroxidase (POX) activity against gray mold disease caused by Botrytis cinerea fungus on Vicia faba L. (bean) leaves was analyzed spectrophotometrically. Stock solutions of distilled water, ethanol and methanol extracts of R. sativus tuber were prepared with dimethyl sulfoxide (DMSO). A spore suspension of 105 spores/mL of B. cinerea was prepared. The leaves of V. faba were treated with distilled water (negative control), only DMSO, only B. cinerea (positive control), only extract treatment (distilled water, ethanol and methanol extract) and B. cinerea applications after extract treatments. The leaves were extracted in accordance with the methods to be analyzed. Analyzes of the supernatants obtained from the extracts were performed spectrophotometrically. As a result of the research, it was determined that the total protein content increased in the extract:fungus treatment groups compared to the group treated with distilled water (negative control). However, it was determined that it decreased in generally compared to the group that was only treated with fungus (positive control). POX activity was found to be increased compared to the negative control group. Total protein content and POX activity increased in only the extract groups compared to the negative control group. The highest POX activity was obtained from the treatment of 10 mgmL distilled water:fungus. In this respect, it can be stated that the best antioxidant activity among the extract:fungus treated groups is 10 mg/mL distilled water:fungus treatment. From this point of view, it can be stated that R. sativus distilled water, ethanol and methanol extracts give an antioxidan defense response in V. faba leaves.

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