ARZU ÖZLÜER HUNT, FERBAL ÖZKAN YILMAZ, Şanser DELİOĞLAN, MEHMET BERKÖZ

Organik ve İnorganik Selenyum Katkılı Yemlerle Beslenen Oreochromis niloticus ’da Antioksidan ve İmmün Sistem Parametrelerinin Araştırılması

Bu çalışmada organik (Sel-Plex®-2000, Alltech, USA) ve inorganik selenyum (Na2SeO3.5H2O - Sodyum selenite pentahydrate-FLUCA) ilaveli yem ile beslenen Oreochromis niloticus'un karaciğer antioksidan enzim aktiviteleri ve immün sistem parametreleri araştırılmıştır. Ortalama ağırlıkları 12,62 ± 0,11 g olan balıklar, 5 farklı uygulama grubu (Kontrol; Org1,5; Org3,0; İnorg1,5; İnorg3,0) olacak şekilde akvaryumlara yerleştirilmiş ve 75 gün beslenmişlerdir. Deneme süresi sonunda karaciğer katalaz (CAT), süperoksit dismutaz (SDO), glutatyon peroksidaz (GSH-Px), lizozim (LYZ) ve miyeloperoksidaz (MPO) aktiviteleri ile malondialdehit (MDA) düzeyi ve kas selenyum seviyeleri belirlenmiştir. Karaciğer antioksidan enzim aktiviteleri ve immün sistem parametreleri Se gruplarında kontrol grubuna oranla artmıştır. Kas dokusu Se düzeyi Se ilaveli tüm gruplarda kontrol ile karşılaştırıldığında göre önemli oranda artmıştır. 3mg/kg organik selenyumla beslenen balıklarda, kas selenyum seviyeleri diğer gruplara oranla daha yüksek bulunmuştur.

Investigation of Antioxidant and Immune System Parameters in Oreochromis niloticus Fed with Organic and Inorganic Selenium Supplementation Feeds

In this study, antioxidant enzyme activities and immun system parameters of liver tissue of Oreochromis niloticus fed with organic (Sel-Plex®-2000, Alltech, USA) and inorganic selenium (Na2SeO3.5H2O - Sodium selenite pentahydrate-FLUCA) supplemented feed were investigated. The fish with average weights of 12.62 ± 0.11 g were placed in aquariums and fed for 75 days in 5 different application groups (Control; Org1.5; Org3.0; Inorg1.5; Inorg3.0). At the end of the trial period, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), lysozyme (LYZ) and myeloperoxidase (MPO) activities and MDA level in the liver tissue and selenium accumulation in the muscle was determined. The antioxidant enzyme activities and immune system parameters in liver were increased in all Se groups compared to control. Se levels in muscle tissues significantly increased in all tested selenium groups when compared to control. In fish fed with 3mg/kg of organic selenium, muscle selenium levels were found to be higher than other groups.

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