Mehmet Burakalp YUSUFLU, Sakine Ülküm ÇİZMECİ

Kültür Medyumuna İlave Edilen Antioksidanların Blastosist Gelişim Oranlarına Etkisi

Sunulan çalışmada in vitro embriyo üretiminde kültür medyumlarına eklenen antioksidanların blastosist gelişim oranlarına etkisinin belirlenmesi amaçlandı. Çalışmanın materyalini mezbahadan alınan ovaryumlardan toplanan oositler oluşturdu. Kumulus oosit kompleksleri (COC) stereo mikroskop altında toplandı ve sınıflandırıldı. Çalışmaya alınacak oositler maturasyon ve fertilizasyon aşamalarına tabi tutuldu. Muhtemel zigotlar antioksidanların (L-ergotiyonin 100 μM (n:163), Vitamin E 100 μM (n:151) Sisteamin 50 μM (n:154) ilave edildiği kültür (IVC) droplarına aktarılarak tri gaz (Hera Cell- %6 O2, %6CO2, %88N) inkübatörde kültüre bırakıldı. Kültür ortamındaki 6. ve 7. gün blastosist oranları ve embriyo kaliteleri değerlee yerleştirildi. IVMFC aşamalarında oluşan farklılıklar Ki-kare testi ile değerlendirildi. Mezbahadan toplanan 162 ovaryumdan 966 adet oosit toplandı. Ovaryum başına oosit sayısının 5,96 olduğu A ve B kalite oosit sayısının ise 4,26 olduğu belirlendi. İn vitro maturasyona alınan toplam 690 oositin 655’inin (%94,93) mature olduğu belirlendi. Grupların cleavage oranları sırasıyla %83,44; %80,79; %79,87 ve %83,96 olduğu belirlendi. Kültür aşamasına alınan 655 oositten 140 (%21,37) adet blastosist elde edildi ve gruplardaki blastosist oranları sırasıyla %33,13; %8,61; %7,79 ve %32,62 olduğu görüldü. Yapılan çalışma sonucunda L-ergotiyonin eklenen gruptaki bilastosist oranlarının kontrol grubu ile benzer olduğu ancak sisteamin ve Vitamin E eklenen gruplarda blastosisit oranlarının önemli oranda düştüğü belirlendi. Bu düşüşün antioksidanların dozu yada oosit gelişim yeterliliğinden etkilenmiş olabileceği düşünüldü.

The Effect of Antioxidants Added to Culture Medium on Blastocyst Development Rates

The present study, it was aimed to determine the effect of antioxidants added to culture media on blastocyst development rates in in vitro embryo production. The material of the study consisted of oocytes collected from the ovaries taken from the slaughterhouse. Cumulus oocyte complexes (COC) were collected and classified under a stereomicroscope. Oocytes included in the study were subjected to maturation and fertilization stages. Probable zygotes were transferred to the culture (IVC) containing antioxidants (L-ergothionine 100 μM (n: 163), Vitamin E 100 μM (n: 151) Cysteamine 50 μM (n: 154) and were cultured in a tri gas incubator (Hera Cell- 6% O2%, 6%CO2, 88%N). Blastocyst rates and embryo quality were determined on the 6th and 7th days in culture medium. Differences in IVMFC stages were evaluated by chi-square test. 966 oocytes were collected from 162 ovaries collected from the slaughterhouse. It was determined that the number of oocytes per ovary was 5,96, and the number of A and B quality oocytes was 4.26. It was determined that 655 (94.93%) of a total of 690 oocytes undergoing in vitro maturation were mature. The cleavage rates of the groups were 83.44%; 80.79%; 79.87%, and 83.96%, respectively. 140 (21.37%) blastocysts were obtained from 655 oocytes taken into the culture stage and the blastocyst rates in the groups were 33.13%; 8.61%; 7.79%, and 32.62%, respectively. As a result of the study, it was determined that the rates of blastocysts in the L-ergothioneine added the group was similar to the control group, but the blastocyst rates decreased significantly in the cysteamine and Vitamin E added groups. It was thought that this decrease might have been affected by the dose of antioxidants or the adequacy of oocyte development

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