How Pullulanase Affects Resistant Starch and Antioxidant Activity in Purple Sweet Potato Powder?

Purple sweet potato (PSP) serves as a potential source for dual functionalities of resistant starch (RS) and antioxidants. This study aims to evaluate the effects of pullulanase enzyme on these functionalities. Results showed that the incorporation of pullulanase into PSP powder could significantly increase the RS content from 3.06 g/100g to 7.11 g/100g. However, total anthocyanin content and DPPH radical scavenging activity reduced significantly, due to the interference from RS fragments on anthocyanins. Securing both functionalities (RS and antioxidant) within the same sample is seemingly impossible. A compromise between RS and antioxidant properties in coloured, starchy plant powders is recommended.

___

Alcalde-Eon C, Escribano-Bailón MT, Santos-Buelga C, RivasGonzalo JC. 2006. Changes in the detailed pigment composition of red wine during maturity and ageing: A comprehensive study. Anal. Chim. Acta 563: 238-254.

Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Technol. 28: 25-30.

Burgos G, Amoros W, Muñoa L, Sosa P, Cayhualla E, Sanchez C, Díaz C, Bonierbale M. 2013. Total phenolic, total anthocyanin and phenolic acid concentrations and antioxidant activity of purple-fleshed potatoes as affected by boiling. J. Food Compost. Anal. 30: 6-12.

Eerlingen R, Delcour J. 1995. Formation, analysis, structure and properties of type III enzyme resistant starch. J Cereal Sci. 22: 129-138.

Englyst H, Wiggins H, Cummings J. 1982. Determination of the non-starch polysaccharides in plant foods by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 107: 307-318.

He F, Liang NN, Mu L, Pan QH, Wang J, Reeves MJ, Duan CQ. 2012. Anthocyanins and their variation in red wines II. Anthocyanin derived pigments and their color evolution. Molecules 17: 1483-1519.

Kim JM, Park SJ, Lee CS, Ren C, Kim SS, Shin M. 2011. Functional properties of different Korean sweet potato varieties. Food Sci. Biotechnol. 20: 1501-1507.

Madzlan K, Hasnisa H, Sabeetha S, Dayana M. 2012. Extraction of starch and enzymatic production of high amylose starch from sweetpotato (Ipomea batatas) var. Telong. J. Trop. Agric. Food Sci. 40: 203-210.

McGee H. 2004. A survey of common vegetables. In: McGee H (ed) On Food and Cooking: The Science and Lore of the Kitchen. 2nd ed. New York, USA: Scribner Publisher, 305.

Megazyme International. 2011. Resistant starch assay procedure: AOAC method 2002.02 and AACC method 32-40.01, Wicklow, Ireland: Megazyme International Ireland.

Miles MJ, Morris VJ, Orford PD, Ring SG. 1985. The roles of amylose and amylopectin in the gelation and retrogradation of starch. Carbohydr. Res. 135: 271-281.

Mulinacci N, Ieri F, Giaccherini C, Innocenti M, Andrenelli L, Canova G, Saracchi M, Casiraghi MC. 2008. Effect of cooking on the anthocyanins, phenolic acids, glycoalkaloids, and resistant starch content in two pigmented cultivars of Solanum tuberosum L. J. Agric. Food Chem. 56: 11830- 11837.

Raigond P, Ezekiel R, Raigond B. 2015. Resistant starch in food: A review. J. Sci. Food Agric. 95: 1968-1978.

Sajilata MG, Singhal RS, Kulkarni PR. 2006. Resistant starch–A review. Compr. Rev. Food Sci. Food Saf. 5: 1-17.

Surendra Babu A, Parimalavalli R. 2018. Effect of pullulanase debranching and storage temperatures on structural characteristics and digestibility of sweet potato starch. Journal of the Saudi Society of Agricultural Sciences 17: 208- 216.

Vasanthan T, Bhatty R. 1998. Enhancement of resistant starch (RS3) in amylomaize, barley, field pea and lentil starches. Starch/Stärke 50: 286-291.

Walter WM, Purcell AE, Nelson AM. 1975. Effects of amylolytic enzymes on “moistness” and carbohydrate changes of baked sweet potato cultivars. J. Food Sci. 40: 793-796.
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)