ZELİHA YILDIRIM, Yaselin İLK, METİN YILDIRIM

Enterosin KP'nin Antibakteriyal Aktivitesi Üzerine Para-Hidroksi-Benzoik Asit ile Propil-Parabenin Sinerjit Etkisi

Bu çalışmada, Enterococcus faecalis KP tarafından üretilen enterosin KPnin inhibitör aktivitesi üzerine gıda koruyucularından p-hidroksi-benzoik asit ve propil-parabenin etkisi incelenmiştir. Test mikroorganizması olarak enterosin KP bakteriyosinine dayanıklı olan Staphylococcus aureus, Escherichia coli O157:H7 ve Salmonella Typhimurium kullanılmıştır. Enterosin KP (1600 AU/ml) tek başına ve p-hidroksi-benzoik asit (%0,1- 0,3) ve propil-paraben (%0,008-0,16) ile birlikte kullanılarak Staphylococcus aureus, Escherichia coli O157:H7 ve Salmonella Typhimuriumun gelişimi üzerine inhibitör aktivitesi belirlenmiştir. Enterosin KP, p-hidroksi-benzoik asit (%0,1-0,3) ve propilparaben (%0,008-0,16) ile birlikte kullanıldığında inhibitör aktivitesinin arttığı ve hatta Staphylococcus aureus, Escherichia coli O157:H7 ve Salmonella Typhimuriumun enterosin KPye karşı duyarlı hale geçtikleri belirlenmiştir. Sonuç olarak, enterosin KP diğer gıda koruyucu sistemleriyle birlikte kullanıldığında inhibitör aktivitesi ve spektrumunun arttığı saptanmıştır.

The Synergist Effect of P-Hydroxybenzoic Acid and Propyl-Paraben on The Antibacterial Activity of Enterocin KP

In this study, the effects of food preservative p-hydroxybenzoic acid and propyl-paraben on the inhibitory activity of enterocin KP produced by Enterococcus faecalis KP were determined. Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella Typhimurium, resistant to enterocin KP bacteriocin, were used as target organisms. The inhibitor activity of enterosin KP (1600 AU/ml) alone or in combination with phydroxybenzoic acid (%0.1-0.3) and propyl-paraben (%0.008-0.16) on the growth of Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella Typhimurium were determined. The inhibitory activity of enterocin KP was increased when used in combination with p-hydroxybenzoic acid and propyl-paraben at concentrations of 0.1- 0.3% and 0.008-0.016%, respectively. Furthermore, Staphylococcus aureus, E. coli O157:H7 and Salmonella Typhimurium became sensitive to enterocin KP. In conclusion, the use of enterocin KP in combination with other food preservatives principles resulted in an increase in its inhibitory activity and spectrum.

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