Elma Bitkisi (Malus domestica L.)'nde Foliar Uygulanan Salisilik Asitin Donmaya Karşı Etkileri

Türkiye ve dünya ekonomisinde önemli bir yeri olan elma’nın donma zararına direncini artırmayı, verim ve kalitesini yükseltmeyi amaçladığımız çalışmamızda elma (Malus domestica L.) bitkilerinde fenolojik ve morfolojik gözlemler ile fizyolojik ve biyokimyasal analizler yapılmıştır. Bu bitkinin donma stresine karşı gösterdiği adaptasyon ile kalite ve verimi üzerine foliar salisilik asitin (0, 500 ppm/bitki ve 1000 ppm/bitki) etkileri belirlenmeye çalışılmıştır. Yaprakta fotosentetik pigment içerikleri, total protein miktarı, prolin miktarı ile süperoksit dismutaz ve peroksidaz enzim aktiviteleri ölçülmüştür. Çalışma tesadüfi deneme desenine göre planlanmış ve verilerin istatistik analizleri SPSS paket programıyla (LSD testi) yapılmıştır. Elma bitkilerinin sürgün ve meyve sayılarının 500 ppm salisilik asit uygulamalarında, meyve ağırlıklarının ise 500 ppm ve 1000 ppm salisilik asit uygulamalarında kontrol grubuna göre artış gösterdiği, aynı zamanda uygulama gruplarında elma renklerinin de koyulaştığı tespit edilmiştir. Kla, klb, toplam kl, prolin, süperoksit dismutaz ve peroksidaz miktarları uygulama gruplarında kontrol grubuna göre istatistiki açıdan önemli derecede artmıştır. Karotenoid ve protein miktarlarında meydana gelen artışlar ise istatistiki açıdan önemli değildir. Elde edilen veriler ışığında foliar salisilik asit uygulamalarının elma bitkisinde donma stresinin etkilerini azalttığı, verim ve kaliteyi artırdığı saptanmıştır.

Effects of Foliar Treatments of Salicylic Acid on Apple (Malus domestica L.) Against Freezing

In our study we aim to increase frost resistance and improve the yield and quality of apple is important in Turkey and world economy. Phenological and morphological observations, physiological and biochemical analyzes were carried out in apple (Malus domestica L.) plants. It was studied to determine the effects of foliar Salicylic acid (0, 500 ppm/plant and 1000 ppm/plant) on adaptation of this plant when exposed to freezing stress, the quality and yield. Leaf photosynthetic pigment contents, total protein amount, proline amount, superoxide dismutase and peroxidase enzymatic activities were measured. The study planned by random experimental design and statistical analysis of data with SPSS program (LSD test) were made. It was determined that fruit and shoot numbers were increased in samples exposed to 500 ppm salicylic acid and while fruit weights were increased in samples exposed to 500 ppm and 1000 ppm salicylic acid compared to the control. Also, colour of plants were darkened. Chla, chlb, total chl, proline, superoxide dismutase and peroxidase amounts were increased significantly compared to the control group. Increases occured in the carotenoid and the protein amounts are not significant statistically. In the light of obtained data, foliar application of salicylic acid were found to reduce the effects of freezing stress and to increase the yield and quality of apple plants.

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  • Aktaş YL. 2001. Vitis vinifera L. cv. Sultani’de salisilik asit uygulamasının yaprak proteinleri içeriği üzerine etkileri. Doktora tezi, Ege Üniv. Fen Bil. Ens., Biyoloji Anabilim Dalı, İzmir.
  • Anjum SA, Farooq M, Xie X, Liu XJ, Ijaz MF. 2012. Antioxidant defense system and proline accumulation enables hot pepper to perform better under drought. Sci. Hort., 140: 66-73.
  • Antikainen M, Griffith M. 1997. Antifreeze protein accumulation in freezing-tolerant cereals. Physiol. Plant., 99: 423–432.
  • Atıcı Ö, Nalbantoğlu B. 1999. Effect of apoplastic proteins on freezing tolerance in leaves. Phytochemistry, 50: 755-761. Atıcı Ö, Nalbantoğlu B. 2003. Antifreeze proteins in higher plants. Phytochemistry, 64: 1187-1196.
  • Aygün A, Şan B. 2005. The late spring frost hardiness of some apple varieties at various stages of flower buds. Ankara Üniv. Tarım Bil. Dergisi, 11: 283-285.
  • Babalar M, Asghari M, Talaei A, Khosroshahi A. 2007. Effect of pre- and postharvest salicylic acid treatment on ethylene production, fungal decay and overall quality of Selva strawberry fruit. Food Chem., 105: 449-453.
  • Bates LS, Waldren RP, Tevre, IU. 1973. Rapid determination of free proline for waterstress studies. Plant and Soil, 39: 205- 207.
  • Beauchamp C, Fridovich I. 1971. Superoxide Dismutase: Improved assay and applicable to acrylamide gels. Anal. Biochem., 44: 276-287.
  • Blokhina O, Virolainen E, Fagerstedt K. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot., 91: 179-194.
  • Bradford M. 1976. A rapid and sensitive method for quantation of microgram quantities of proteins utilizing the principle of protein dye binding. Anal. Biochem., 72: 248-250.
  • El-Bahy MM. 2002. Metabolic changes, phytohormonal level and activities of certain related enzymes associated with growth of presoaked lupine seeds in salicylic and gallic acid. Bull. Fac. Sci., Assuit Univ., 31: 259-270.
  • El-Tayeb MA. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regul., 45: 215-224.
  • Fariduddin Q, Hayat S, Ahmda A. 2003. Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity, and seed yield in Brassica juncea. Photosynthetica, 41: 281-284.
  • Farooq M, Aziz T, Basra SMA, Cheema MA, Rehman H. 2008. Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. J. of Agron. and Crop Sci., 194: 161-168.
  • Foyer CH, Fletcher JM. 2001. Plant antioxidants: colour me healthy. Biologist, 48: 115-120.
  • Ghasemzadeh A, Jaafar HZE. 2013. Interactive effect of salicylic acid on some physiological features and antioxidant enzymes activity in Ginger (Zingiber officinale Roscoe). Molecules, 18: 5965-5979.
  • Griffith M, Yaish MWF. 2004. Antifreeze proteins in overwintering plants: a tale of two activities. Trends Plant Sci., 9: 399-405.
  • Gunes A, Inal A, Alpaslan M, Eraslan F, Bagci EG, Cicek N. 2007. Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. J. Plant Physiol., 164: 728-736.
  • Halliwell B, Gutteridge JMC. 1999. Free radicals in biology and medicine. Oxford University Press, New York.
  • Herzog V, Fahimi H. 1973. Determination of the activity of peroxidase. Anal. Biochemistry, 55: 554-562.
  • Hua YJ, Yuan G, Man LY, Hua QX, Fang ZM. 2008. Salicylic acid-induced enhancement of cold tolerance through activation of antioxidative capacity in watermelon. Scientia Hort., 118: 200-205.
  • Kacar B, Katkat V, Öztürk Ş. 2002. Bitki Fizyolojisi, Uludağ Üniv. Güçlendirme Vakfı, Yayın no: 198, Bursa, s. 493, 494, 510-513.
  • Kalarani MK, Thangaraj M, Sivakumar R, Mallika V. 2002. Effects of salicylic acid on tomato (Lycopersicon esculentum Mill) productivity. Crop Res. (Hisar), 23: 486- 492.
  • Kang G, Wang C, Sun G, Wang Z. 2003. Salicylic acid changes activities of H2O2
  • -metabolizing enzymes and increases the chilling tolerance of banana seedlings. Environ. and Exp. Bot., 50: 9-15.
  • Karlidag H, Yildirim E, Turan M. 2009. Exogenous applications of salicylic acid affect quality and yield of strawberry grown under antifrost heated greenhouse conditions. J. Plant Nutr. Soil Sci., 172: 270-276.
  • Kaya C, Tuna AL, Ashraf M, Altunlu H. 2007. Improved salt tolerance of melon (Cucumis melo L.) by the addition of proline and potassium nitrate. Environ. Exp. Bot., 60: 397- 403.
  • Kusvuran S, Ellialtioglu S, Polat Z. 2013. Antioxidative enzyme activity, lipid peroxidation, and proline accumulation in the callus tissues of salt and drought tolerant and sensitive pumpkin genotypes under chilling stres. Hort. Environ. Biotechnol., 54: 319-325.
  • Lu JF, Yu JH. 2004. Effects of SA on physiological indexes of chilling-tolerance in watermelon seedlings. J. of Gansu Agric. Univ.
  • Pell EJ, Dann MS. 1991. Multiple stress and plant senescence. In: H. A.Mooney, W. E.Winner, and E.J.Pell, eds. Integrated Response of Plants to Stress, pp. 189-284, Academic Press, San Diego, CA.
  • Raskin I. 1992. Role of salicylic acid in plants. Ann. Rev. Plant Physiol. Molec. Biol., 43: 439-463.
  • Reyes, E, Jennings PH. 1994. Response of cucumber (Cucumis sativus L.) and squash (Cucurbita pepo L. var. melopepo) roots to chilling stress during early stages of seedling development. J. Am. Soc. Hort. Sci. 119:964-970.
  • Sayyari M. 2012. Improving chilling resistance of cucumber seedlings by salicylic acid. American-Eurasian J. Agric. and Environ. Sci., 12: 204-209.
  • Sayyari M, Ghanbari F, Fatahi S, Bavandpour F. 2013. Chilling tolerance improving of watermelon seedling by salicylic acid seed and foliar application. Not. Sci. Biol., 5(1): 67-73. Scandalios JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiol., 101:7-12.
  • Tasgin E, Atici Ö, Nalbantoglu B. 2003. Effect of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regul., 41: 231-236.
  • Türkyılmaz B, Aktaş L, Güven A. 2005. Phaseolus vulgaris L.’te salisilik asit uyarımlı bazı fizyolojik ve biyokimyasal değişimler. Fırat Üniv., Fen ve Müh. Bil. Dergisi, 17: 319- 326.
  • Witham FH, Blayles DF, Devlin RM. 1971. Experiments in plant physiology. Van Nostrand Reinheld Company, New York, pp. 55-56.
  • Yan DY, Cheng WP, Jia C, Peng SC. 2008. Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana, (Special Issue: Understanding abiotic stresses and the solution), J. Integrative Plant Biol., 5: 1318- 1326.
  • Yildirim E, Guvenc I, Karatas A. 2006. Effect of different number foliar salicylic acid applications on plant growth and yield of cucumber, VI. Turkey National Vegetable symposium, Kahramanmaras, Turkey, September 19–22.
  • Yordanova R, Popova L. 2007. Effect of exogenous treatment wıth salicylic acid on photosynthetic activity and antioxidant capacity of chilled wheat plants. Gen. Appl. Plant Physiolog., 33: 155-170.
  • Yu XM, Griffith M, Wiseman SB. 2001. Ethylene induces antifreeze activity in winter rye leaves. Plant Physiol., 126: 1232-1240.
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)
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