Polifenol Oksidaz Enzimi ve İnaktivasyon Yöntemleri

Polifenol oksidaz enzimi, sebze ve meyvelerde bulunmasının yanı sıra bazı hayvansal organlarda ve mikroorganizmalarda da bulunmaktadır. Enzimatik esmerleşmeden sorumlu olan polifenol oksidaz enzimi meyve ve sebzelerde yaygın olarak bulunan fenolik bileşiklerin kahverengi pigmentleri üreten kinonlara oksidasyonunu katalizleyen bakır proteinlerinin bir grubudur. Meyve ve sebzelerin endüstriyel olarak hazırlanması sırasında polifenol oksidazın katalitik etkisi sonucu enzimatik esmerleşme meydana gelmektedir. Enzimatik esmerleşme fenolik yapıda bileşenler içeren ürünlerde görünüşü bozmakla kalmayıp istenmeyen renk, koku ve tat oluşumuna ve ürünlerin besleyici değerinde önemli ölçüde kayıplara neden olmaktadır. Bu durum ürünlerin tüketici tarafından kabul edilirliğini etkilemekte ve ekonomik kayıplara neden olmaktadır. Bu derlemede farklı meyve ve sebzelerdeki polifenol oksidaz enziminin bazı karakteristikleri gözden geçirilmiş ve polifenol oksidazı inaktive etmek için kullanılan enzimatik esmerleşme karşıtı kimyasal ajanlar, ısıl uygulamalar, ışınlama uygulamaları ve yüksek basınç uygulaması, vurgulu elektrik alan uygulaması, süperkritik karbondioksit uygulaması ve ultrason uygulaması gibi alternatif yöntemler hakkında bilgi verilmiştir.

Polyphenol Oxidase Enzyme and Inactivation Methods

Polyphenol oxidase enzyme is found in vegetables and fruits, as well as in some animal organs and microorganisms. Polyphenol oxidase enzyme responsible for enzymatic browning is a group of copper proteins that catalyses the oxidation of phenolic compounds to quinones, which produce brown pigments, commonly found in fruits and vegetables. During the industrial preparation of fruits and vegetables, results of catalytic effect of polyphenol oxidase causes enzymatic browning. Enzymatic browning impairs the appearance of products containing phenolic compounds along with undesirable colour, odor and taste formation and significant loss of nutritional value of the products. This affects the acceptability of the products by the consumers and causes economic losses. In this review, some characteristics of polyphenol oxidase enzyme in different fruits and vegetables have been reviewed and information about chemical antibrowning agents, thermal applications, irradiation applications and alternative methods such as high pressure processing, pulse electric field, supercritical carbon dioxide and ultrasound applications to inactivate this enzyme has been presented.

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