Ida ² GİARDİNO, Massimo Pettoello- MANTOVANİ, Angelo CAMPANOZZİ, Maria D'APOLİTO

İnek sütü proteini alerjisi olan çocukların periferik kan mononükleer hücrelerinde inflamatuar sitokin düzeyler

Amaç: Çalışmanın amacı, inek sütü proteini alerjisi olan hastaların inek sütü proteini ile uyarılmış periferik mononükleer hücrelerinin kültürlerinde sitokin düzeylerini incelemekti. Gereç ve Yöntemler: İnek sütü proteini alerjisi olan 11 çocuk ve alerjisi olmayan 11 kontrol incelendi. Bu çocukların periferik kan mononükleer hücreleri tek başına ve inek sütü ?-laktalbümin; ?-laktoglobülin; ?S 1, ?S 2, ? ve ?-kazein fraksiyonu karışımları ve tam sütten elde edilen bir inek sütü protein karışımı varlığında kültüre edildi. Kültür süzüntülerinde, sitokinlerin (tümör nekroz faktörü-?, interlökin-10 ve interlökin-12) düzeyleri belirlendi.Bulgular: İnek sütü proteini alerjisi olan çocukların inek sütü proteini ile uyarılmış periferik kan mononükleer hücre kültürlerinde; tümör nekroz faktörü-?, interlökin-10 ve interlökin-12 düzeyleri, alerjisi olmayan kontrollere göre anlamlı derecede daha yüksekti (p

Levels of inflammatory cytokines from peripheral blood mononuclear cells of children with cow’s milk protein allergy

Aim: The aim of the study was to investigate the level of cytokines in cultures of cow’s milk protein- stimulated peripheral blood mononuclear cells of patients with cow’s milk protein allergy. Material and Methods: Eleven children with cow’s milk protein allergy and 11 non-allergic controls were studied. Their peripheral blood mononuclear cells were cultured alone and in the presence of cow’s milk α-lactalbumin; β-lactoglobulin; αS 1, αS 2, β, and κ-casein fraction mixtures; and a cow’s protein mixture from whole milk. Production of cytokines, tumor necrosis factor-α, interleukin-10, and interleukin-12 were determined in culture supernatants.Results: In cow’s milk protein-stimulated peripheral blood mononuclear cell cultures of children with cow’s milk protein allergy, tumor necrosis factor-α, interleukin-10, and interleukin-12 production was significantly higher than in non-allergic controls (p

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