Metin KILINÇ, Alptekin YASIM, Davut ÖZBAĞ, Harun ÇIRALIK, İsmail TORU

The effect of diosmin-hesperidin combination treatment on the lipid profile and oxidative-antioxidative system in high-cholesterol diet-fed rats

Amaç: Bu çalışmada yüksek kolesterollü diyetle beslenen sıçanlarda diosmin-hesperidin kombinasyonu tedavisinin serum lipid profili ile oksidatif-antioksidatif sistem üzerindeki etkisi araştırıldı.Çalışma planı: Çalışmaya ağırlıkları 220 ile 280 g arasında değişen 36 Sprague-Dawley cinsi sıçan alındı ve sıçanlar her bir grupta 12 sıçan olacak şekilde rasgele biçimde üç gruba ayrıldı. Kontrol grubundaki sıçanlar (Grup 1) standart sıçan yemi ile beslenirken, grup 2 (Yüksek kolesterollü diyet grubu) ve grup 3'teki (100 mg/kg/gün diosmin-hesperidin grubu) sıçanlar üç ay boyunca yüksek kolesterollü diyetle beslendi. Çalışma periyodu sonunda serumda total kolesterol, düşük yoğunluklu lipoprotein (LDL) kolesterol, yüksek yoğunluklu lipoprotein (HDL) kolesterol ve ve trigliserid düzeyleri ölçüldü. Serumda ve kalp dokusu örneklerinde malondialdehit (MDA), glutatyon peroksidaz (GPX), süperoksit dismutaz (SOD) ve katalaz düzeyleri ölçüldü.Bulgular: Total kolesterol, LDL-kolesterol ve trigliserid düzeyleri açısından gruplar arasında anlamlı farklılık yoktu. Yüksek yoğunluklu lipoprotein kolesterol düzeyi ise grup 3'te, grup 1 ve 2'dekinden anlamlı ölçüde yüksekti. Malondialdehit düzeyleri açısından gruplar arasında anlamlı farklılık yoktu. Glutatyon peroksidaz ve SOD düzeylerinin grup 3'te grup 1 ve 2'ye göre anlamlı ölçüde yüksek olduğu saptandı. Katalaz düzeyleri açısından gruplar arasında hiçbir anlamlı farklılık yoktu. İntrakardiyak MDA, GPX, SOD ve katalaz ölçümlerinin gruplar arasında anlamlı ölçüde farklı olmadığı görüldü.Sonuç: Yüksek kolesterollü diyetle beslenen sıçanlara diosminhesperidin kombinasyonu verilmesi HDL-kolesterol düzeylerini anlamlı ölçüde artırdı, fakat diğer lipid parametrelerini anlamlı düzeyde etkilemedi. Oksidatif-antioksidatif dengede olumlu değişiklikler meydana geldi ve diosmin-hesperidin uygulaması GPX ve SOD düzeylerinde anlamlı ölçüde artış sağlandı.

Yüksek kolesterollü diyetle beslenen sıçanlarda diosmin-hesperidin kombinasyonu tedavisinin lipid profili ve oksidatif-antioksidatif sistem üzerindeki etkisi

Background: In this study, the effect of diosmin-hesperidin combination treatment on serum lipid profile and oxidativeantioxidative system in high-cholesterol diet-fed rats was investigated.Methods: Thirty-six Sprague-Dawley rats, weighing between 220 and 280 g, were included in this study and were randomly assigned to three groups with 12 rats in each group. While rats in the control group (Group 1) were fed standard rat chow diet, those in group 2 (High cholesterol diet group) and group 3 (100 mg/kg/day diosmin-hesperidin group) were fed high-cholesterol diet for three months. After the study period, the levels of total cholesterol, low-density lipoprotein (LDL) cholesterol, highdensity lipoprotein (HDL) cholesterol and triglycerides were measured in serum. Malondialdehyde (MDA), glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase levels were measured in serum and heart tissue samples.Results: The levels of total cholesterol, LDL-cholesterol and triglyceride were not significantly different between the groups. High-density lipoprotein cholesterol level was found to be significantly higher in group 3 than that in groups 1 and 2. Malondialdehyde levels were not significantly different between the groups. Glutathione peroxidase and SOD levels were found to be significantly higher in group 3 than those in groups 1 and 2. There were no significant differences between the groups with respect to catalase levels. Intracardiac measurements of MDA, GPX, SOD and catalase were not found significantly different between the groups.Conclusion: Diosmin-hesperidin administration to high-cholesterol diet-fed rats significantly increased HDL-cholesterol levels but did not significantly affect other lipid parameters. Positive changes occurred in oxidative-antioxidative balance and administration of diosmin-hesperidin significantly increased the levels of GPX and SOD.

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