Tünay KURTOĞLU, Selim DURMAZ, Ömer Faruk RAHMAN, Nesibe ÇETİN, Mustafa YILMAZ, Emin BARBARUS, Muhammet Hüseyin ERKAN

Borate reduces experimental supra-celiac aortic clamping-induced oxidative stress in lung and kidney, but fails to prevent organ damage

Background: This study aims to investigate the effects of 2-aminoethoxydiphenyl borate (2-APB) on aortic clamping-induced lung and kidney tissue oxidation, tissue inflammation, and histological damage in a rat model. Methods: A total of 28 adult female Wistar albino rats were randomly allocated to four equal groups: Control group, ischemia-reperfusion group, dimethyl sulfoxide group, and 2-APB group. Animals in the control group underwent median laparotomy. In the remaining groups, supra-celiac aorta was clamped for 45 min and, then, reperfusion was constituted for 60 min. The 2-APB (2 mg/kg) was administered before clamping. The remaining groups received saline (ischemia-reperfusion group) or dimethyl sulfoxide (dimethyl sulfoxide group). Kidney and lung tissue samples were harvested at the end of reperfusion. Results: Aortic occlusion caused increased tissue total oxidant status and reduced total antioxidant status and glutathione levels in the ischemia-reperfusion and dimethyl sulfoxide groups. Tissue interleukin-1 beta and tumor necrosis factor-alpha levels, nuclear factor kappa beta activation, and histological damage severity scores were also higher in these groups. The 2-APB treatment eliminated the increase in total oxidant status and the decrease in total antioxidant status and glutathione levels. It also caused a decrease in the interleukin-1 beta levels, although it did not significantly alter the tumor necrosis factor-alpha levels, nuclear factor kappa beta immunoreactivity, and histological damage scores. Conclusion: Borate exerted a beneficial antioxidant effect as evidenced by reduced oxidative stress; however, it did not inhibit nuclear factor kappa beta activation and prevent histological damage in supra-celiac aortic clamping-induced kidney and lung injury in rats

Borat akciğer ve böbrekte deneysel supraçölyak aort klemplenmesine bağlı oksidatif stresi azaltır, ancak organ hasarını önlemekte yetersizdir

Amaç: Bu çalışmada sıçan modelinde 2-aminoetoksidifenil boratın (2-APB) aortik klemplenmeye bağlı akciğer ve böbrek dokusu oksidasyonu, doku enflamasyonu ve histolojik hasar üzerindeki etkileri araştırıldı. Çalışma planı: Toplam 28 erişkin dişi Wistar albino sıçan, rastgele dört eşit gruba ayrıldı: Kontrol grubu, iskemi-reperfüzyon grubu, dimetil sülfoksit grubu ve 2-APB grubu. Kontrol grubundaki hayvanlara medyan laparotomi yapıldı. Kalan gruplarda supraçölyak aort 45 dk. süreyle klemplendi ve ardından 60 dk. reperfüzyon oluşturuldu. Klemplemeden önce 2-APB (2 mg/kg) uygulandı. Diğer gruplarda hayvanlara salin (iskemi-reperfüzyon grubu) veya dimetil sülfoksit (dimetil sülfoksit grubu) verildi. Reperfüzyon sonunda böbrek ve akciğer doku örnekleri alındı. Bulgular: Aortun klemplenmesi, iskemi-reperfüzyon ve dimetil sülfoksit gruplarında dokudaki toplam oksidan statüsünün artmasına ve total antioksidan statüsünün ve glutatyon düzeylerinin azalmasına neden oldu. Doku interlökin-1 beta ve tümör nekroz faktör-alfa düzeyleri, nükleer faktör kappa beta aktivasyonu ve histolojik hasar şiddeti skorları da bu gruplarda daha yüksek bulundu. 2-APB tedavisi, toplam oksidan statüsündeki artışı ve total antioksidan statüsü ve glutatyon düzeylerindeki düşüşü ortadan kaldırdı. Ayrıca, interlökin-1 beta düzeylerinde anlamlı azalmaya neden oldu; ancak tümör nekroz faktör-alfa düzeylerini, nükleer faktör kappa beta aktivasyonunu ve histolojik hasar skorlarını önemli ölçüde değiştirmedi. Sonuç: Borat, sıçanlarda supraçölyak aortik klemplenmeye bağlı böbrek ve akciğer hasarında oksidatif stresi azaltarak faydalı bir antioksidan etki göstermiştir; ancak, nükleer faktör kappa beta aktivasyonunu inhibe etmemiş ve histolojik hasarı önleyememiştir.

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