İbrahim Ethem Özsoy, Mehmet Akif Tezcan, Çiğdem Karakükçü, Fatih Gürler

Primer spontan pnömotoraks etiyolojisinde telomerazın rolü

Amaç: Bu çalışmada göğüs cerrahisi pratiğinde sıklıkla karşılaşılan primer spontan pnömotoraks riskinde telomeraz aktivitesinin rolü araştırıldı. Çalışma planı: Bu prospektif çalışmaya Ocak 2018 - Ağustos 2018 tarihleri arasında primer spontan pnömotoraks nedeniyle tedavi edilen toplam 61 hasta (56 erkek, 5 kadın; medyan yaş: 29.4 yıl; dağılım, 17-43 yıl) ve yaş ve cinsiyet açısından eşleştirilmiş 19 sağlıklı kontrol (10 erkek, 9 kadın; medyan yaş: 29.1 yıl; dağılım, 23-43 yıl) alındı. Telomeraz aktivitesi enzim bağlı immünosorbent testi ile değerlendirildi. Telomeraz aktivitesi ile klinik ve demografik parametreler arasındaki ilişki incelendi. Bulgular: Ortalama serum telomeraz düzeyleri primer spontan pnömotoraks grubunda 3.4±0.6 ng/mL ve kontrol grubunda 1.9±0.5 ng/mL olup, hasta grubunda düzeyler anlamlı ölçüde daha yüksekti (p

The role of telomerase in the etiology of primary spontaneous pneumothorax

Background: This study aims to investigate the role of telomerase activity in the risk of primary spontaneous pneumothorax, which is most frequently encountered in the practice of thoracic surgery. Methods: A total of 61 patients (56 males, 5 females; median age: 29.4 years; range, 17 to 43 years) who underwent treatment for primary spontaneous pneumothorax and 19 age- and sex-matched healthy controls (10 males, 9 females; median age: 29.1 years; range, 23 to 43 years) were included in this prospective study between January 2018 - August 2018. Telomerase activity was evaluated with enzyme-linked immunosorbent assay. The correlation between telomerase activity and clinical and demographic parameters was examined. Results: The mean serum telomerase level was 3.4±0.6 ng/mL in the primary spontaneous pneumothorax group and 1.9±0.5 ng/mL in the control group, indicating significantly higher levels in the patient group (p

PDF

___

1. Kaminker PG, Kim SH, Desprez PY, Campisi J. A novel form of the telomere-associated protein TIN2 localizes to the nuclear matrix. Cell Cycle 2009;8:931-9.
2. Wood AM, Laster K, Rice EL, Kosak ST. A beginning of the end: New insights into the functional organization of telomeres. Nucleus 2015;6:172-8.
3. Cusanelli E, Chartrand P. Telomeric noncoding RNA: Telomeric repeat-containing RNA in telomere biology. Wiley Interdiscip Rev RNA 2014;5:407-19.
4. Ouellette MM, McDaniel LD, Wright WE, Shay JW, Schultz RA. The establishment of telomerase-immortalized cell lines representing human chromosome instability syndromes. Hum Mol Genet 2000;9:403-11.
5. Olovnikov AM. A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon. J Theor Biol 1973;41:181-90.
6. Calado RT, Young NS. Telomere diseases. N Engl J Med 2009;361:2353-65. 7. Kircher LT, Swartzel RL. Spontaneous pneumothorax and its treatment. JAMA 1954;155:24-29.
8. Young NS. Telomere biology and telomere diseases: Implications for practice and research. Hematology Am Soc Hematol Educ Program 2010;2010:30-5.
9. Garcia CK, Wright WE, Shay JW. Human diseases of telomerase dysfunction: Insights into tissue aging. Nucleic Acids Res 2007;35:7406-16.
10. Tschopp JM, Bintcliffe O, Astoul P, Canalis E, Driesen P, Janssen J, et al. ERS task force statement: Diagnosis and treatment of primary spontaneous pneumothorax. Eur Respir J 2015;46:321-35.
11. Olesen WH, Titlestad IL, Andersen PE, Lindahl-Jacobsen R, Licht PB. Incidence of primary spontaneous pneumothorax: A validated, register-based nationwide study. ERJ Open Res 2019;5:00022-2019.
12. Melton LJ 3rd, Hepper NG, Offord KP. Incidence of spontaneous pneumothorax in Olmsted County, Minnesota: 1950 to 1974. Am Rev Respir Dis 1979;120:1379-82.
13. Bense L, Eklund G, Wiman LG. Smoking and the increased risk of contracting spontaneous pneumothorax. Chest 1987;92:1009-12.
14. Gupta D, Hansell A, Nichols T, Duong T, Ayres JG, Strachan D. Epidemiology of pneumothorax in England. Thorax 2000;55:666-71.
15. Bobbio A, Dechartres A, Bouam S, Damotte D, Rabbat A, Régnard JF, et al. Epidemiology of spontaneous pneumothorax: Gender-related differences. Thorax 2015;70:653-8.
16. Çelik B, Nadir A, Şahin E, Kaptanoğlu M, Demir H, Furtun K. Nüks spontan pnömotorakslı olgularda risk faktörleri, klinik ve radyolojik değerlendirme. Turk Gogus Kalp Dama 2008;16:107-12.
17. Lippert HL, Lund O, Blegvad S, Larsen HV. Independent risk factors for cumulative recurrence rate after first spontaneous pneumothorax. Eur Respir J 1991;4:324-31.
18. Gardner M, Bann D, Wiley L, Cooper R, Hardy R, Nitsch D, et al. Gender and telomere length: Systematic review and meta-analysis. Exp Gerontol 2014;51:15-27.
19. Sadikot RT, Greene T, Meadows K, Arnold AG. Recurrence of primary spontaneous pneumothorax. Thorax 1997;52:805-9.
20. Huang TW, Lee SC, Cheng YL, Tzao C, Hsu HH, Chang H, et al. Contralateral recurrence of primary spontaneous pneumothorax. Chest 2007;132:1146-50.
21. Mitlehner W, Friedrich M, Dissmann W. Value of computer tomography in the detection of bullae and blebs in patients with primary spontaneous pneumothorax. Respiration 1992;59:221-7.
22. Armanios M. Telomerase and idiopathic pulmonary fibrosis. Mutat Res 2012;730:52-8.
23. Armanios M, Chen JL, Chang YP, Brodsky RA, Hawkins A, Griffin CA, et al. Haploinsufficiency of telomerase reverse transcriptase leads to anticipation in autosomal dominant dyskeratosis congenita. Proc Natl Acad Sci U S A 2005;102:15960-4.
24. Guo Y, Xie C, Rodriguez RM, Light RW. Factors related to recurrence of spontaneous pneumothorax. Respirology. 2005;10:378-84.
25. Sayar A, Kök A, Citak N, Metin M, Büyükkale S, Gürses A. Size of pneumothorax can be a new indication for surgical treatment in primary spontaneous pneumothorax: A prospective study. Ann Thorac Cardiovasc Surg 2014;20:192-7.
26. Latifovic L, Peacock SD, Massey TE, King WD. The influence of alcohol consumption, cigarette smoking, and physical activity on leukocyte telomere length. Cancer Epidemiol Biomarkers Prev 2016;25:374-80.
27. Feng F, Yang Y, Li Z, Song J, Zhu H, Wang L, et al. Changes in telomere length and telomerase activity in human bronchial epithelial cells induced by coal tar pitch extract, Toxicology Research 2015;4:1535-44.
28. Bense L, Wiman LG. Time relation between sale of cigarettes and the incidence of spontaneous pneumothorax. Eur J Respir Dis 1987;71:362-4.
29. Carrero JJ, Stenvinkel P, Fellström B, Qureshi AR, Lamb K, Heimbürger O, et al. Telomere attrition is associated with inflammation, low fetuin-A levels and high mortality in prevalent haemodialysis patients. J Intern Med 2008;263:302-12.
30. Chung HY, Kim HJ, Kim KW, Choi JS, Yu BP. Molecular inflammation hypothesis of aging based on the anti- aging mechanism of calorie restriction. Microsc Res Tech 2002;59:264-72.
31. Tueller EE, Crise NR, Belton JC, McLaughlin RF Jr. Idiopathic spontaneous pneumothorax. Electron microscopic study. Chest 1977;71:419-21.
32. Akyıl M, Tezel Ç, Evman S, Tokgöz Akyıl F, Vayvada M, Bayram S, et al. Correlation between meteorological changes and primary spontaneous pneumothorax: Myth or fact? Turk Gogus Kalp Dama 2018;26:436-40.
33. Chan-Yeung M, Malo JL. Aetiological agents in occupational asthma. Eur Respir J 1994;7:346-71.