Burhan APİLİOĞULLARI, Ali YÜCEAKTAŞ, Banu YOLDAŞ, Recep KEŞLİ, Hıdır ESME

Akciğer iskemi reperfüzyonunun tetiklediği böbrek hasarında karnosol ön koşullaması

Amaç: Bu deneysel çalışmada, akciğer iskemi/reperfüzyonu (İ/R) ile indüklenen böbrek hasarı üzerine karnosolun koruyucu etkisi araştırıldı.Çalışma planı: Yirmi dört Yeni Zelanda beyaz tavşanı üç gruba randomize edildi. Grup IR; akciğer İ/R grubu (60 dk. iskemi-60 dakika reperfüzyon), grup CIR; akciğer İ/R'si öncesi karnasol bolus enjeksiyonu ve grup S; plasebo grubu (pulmoner hilus klemplenmedi). Böbrek dokusu miyeloperoksidaz (MPO) ve interselüler adezyon molekülü -1 (ICAM-1) düzeyleri değerlendirildi.Bul gu lar: Böbrek dokusu MPO ve ICAM-1 düzeyleri grup IR'de, grup CIR ve grup S ile karşılaştırıldığında, anlamlı olarak daha yüksek idi (sırasıyla, p=0.021 ve p=0.0001). Grup CIR ve grup S arasında ise ölçülen parametrelerde istatistiksel olarak anlamlı fark tespit edilmedi.So nuç: Çalışma sonuçlarımız akciğer İ/R'sine bağlı böbrek hasarında aktif nötrofil sekestrasyonu ile ilişkili MPO ve ICAM-1 düzeylerinin arttığını ve karnosolun bu hasarda koruyucu etkisi olabileceğini göstermektedir

Pretreatment with carnosol in lung ischemia reperfusion-induced renal injury

Background: This experimental study aims to investigate the protective effects of carnosol on kidney injury induced by lung ischemia/reperfusion (I/R). Methods: Twenty-four New Zealand white rabbits were randomized into three groups. Group IR; lung I/R group (60 min ischemia-60 min reperfusion), group CIR; bolus injection of carnosol before lung I/R, and group S; sham group (pulmonary hilum was not clamped). Renal tissue myeloperoxidase (MPO) and intercellular adhesion molecule-1 (ICAM-1) levels were evaluated. Results: Renal tissue MPO and ICAM-1 levels were significantly higher in the group IR compared to the group CIR and group S (p=0.021 and p=0.0001 respectively). No statistically significant difference in the parameters evaluated was detected between the group CIR and the group S. Conclusion: Our study result suggests that lung I/R injury causes increased renal tissue MPO and ICAM-1 levels, which are related to activated neutrophil sequestration and carnosol may play a protective role against this kidney injury

PDF

___

1. de Perrot M, Liu M, Waddell TK, Keshavjee S. Ischemiareperfusion-induced lung injury. Am J Respir Crit Care Med 2003;167:490-511.
2. Yücel O, Güler A, Şirin Y, Gamsızkan M, Şahin MA, Eken A, et al. Akciğer Easy Applicable Model of Ischemia and Reperfusion on Lung Transplantation. J Clin Anal Med 2010;1:13-20.
3. Wang M, Ji P, Wang R, Zhao L, Xia Z. TRPV1 agonist capsaicin attenuates lung ischemia-reperfusion injury inrabbits. J Surg Res 2012;173:153-60.
4. Pazoki-Toroudi HR, Ajami M, Habibey R. Pre-medication and renal pre-conditioning: a role for alprazolam, atropine, morphine and promethazine. Fundam Clin Pharmacol 2010;24:189-98.
5. Park KM, Han HJ. Prior ischemic treatment renders kidney resistant to subsequent ischemia. J Vet Sci 2002;3:115-22.
6. Seifi B, Kadkhodaee M, Delavari F, Mikaeili S, Shams S, Ostad SN. Pretreatment with pentoxifylline and N-acetylcysteine in liver ischemia reperfusion-induced renal injury. Ren Fail 2012;34:610-5.
7. Yuksel S, Sezer MT, Sahin O, Sutcu R, Koçogullari C, Yilmaz HR, et al. The role of carnitine in preventing renal damage developed as a result of infrarenal aortic ischemiareperfusion. Ren Fail 2011;33:440-9.
8. Oksuz H, Bulbuloglu E, Senoglu N, Ciralik H, Yuzbasioglu MF, Kilinc M, et al. Re-protective effects of pre- and post-laparoscopy conditioning, zinc, pentoxifylline, and N-acetylcysteine in an animal model of laparoscopyinduced ischemia/reperfusion injury of the kidney. Ren Fail 2009;31:297-302.
9. Stokes KY, Abdih HK, Kelly CJ, Redmond HP, BouchierHayes DJ. Thermotolerance attenuates ischemia-reperfusion induced renal injury and increased expression of ICAM-1. Transplantation 1996;62:1143-9.
10. Zhang Q, Huang C, Meng B, Tang T, Shi Q, Yang H. Acute effect of ghrelin on ischemia/reperfusion injury in the rat spinal cord. Int J Mol Sci 2012;13:9864-76.
11. Eren S, Esme H, Balci AE, Cakir O, Buyukbayram H, Eren MN, et al. The effect of aprotinin on ischemia-reperfusion injury in an in situ normothermic ischemic lung model. Eur J Cardiothorac Surg 2003;23:60-5.
12. Li JY, Yin HZ, Gu X, Zhou Y, Zhang WH, Qin YM. Melatonin protects liver from intestine ischemia reperfusion injury in rats. World J Gastroenterol 2008;14:7392-6.
13. Gedik HS, Korkmaz K, Erdem H, Karakilic E, Lafci G, Ankarali H. Protective effect of heparin in the end organ ischemia/reperfusion injury of the lungs and heart. J Cardiothorac Surg 2012;7:123.
14. Esme H, Fidan H, Koken T, Solak O. Effect of lung ischemia- -reperfusion on oxidative stress parameters of remote tissues. Eur J Cardiothorac Surg 2006;29:294-8.
15. Poeckel D, Greiner C, Verhoff M, Rau O, Tausch L, Hörnig C, et al. Carnosic acid and carnosol potently inhibit human 5-lipoxygenase and suppress pro-inflammatory responses of stimulated human polymorphonuclear leukocytes. Biochem Pharmacol 2008;76:91-7.
16. Yao JH, Zhang XS, Zheng SS, Li YH, Wang LM, Wang ZZ, et al. Prophylaxis with carnosol attenuates liver injury induced by intestinal ischemia/reperfusion. World J Gastroenterol 2009;15:3240-5.
17. Huang SC, Ho CT, Lin-Shiau SY, Lin JK. Carnosol inhibits the invasion of B16/F10 mouse melanoma cells by suppressing metalloproteinase-9 through down-regulating nuclear factorkappa B and c-Jun. Biochem Pharmacol 2005;69:221-32.
18. Lee JJ, Jin YR, Lim Y, Hong JT, Kim TJ, Chung JH, et al. Antiplatelet activity of carnosol is mediated by the inhibition of TXA2 reeptor and cytosolic calcium mobilization. Vascul Pharmacol 2006;45:148-53.
19. Matute-Bello G, Frevert CW, Martin TR. Animal models of acute lung injury. Am J Physiol Lung Cell Mol Physiol 2008;295:379-99.
20. den Hengst WA, Gielis JF, Lin JY, Van Schil PE, De Windt LJ, Moens AL. Lung ischemia-reperfusion injury: a molecular and clinical view on a complex pathophysiological process. Am J Physiol Heart Circ Physiol 2010;299:H1283-99.
21. Parks DA, Granger DN. Contributions of ischemia and reperfusion to mucosal lesion formation. Am J Physiol 1986;250:G749-53.
22. Balciunas M, Bagdonaite L, Samalavicius R, Baublys A. Markers of endothelial dysfunction after cardiac surgery: soluble forms of vascular-1 and intercellular-1 adhesion molecules. Medicina (Kaunas) 2009;45:434-9.
23. Cowley HC, Heney D, Gearing AJ, Hemingway I, Webster NR. Increased circulating adhesion molecule concentrations in patients with the systemic inflammatory response syndrome: a prospective cohort study. Crit Care Med 1994;22:651-7.
24. Johnson JJ. Carnosol: a promising anti-cancer and antiinflammatory agent. Cancer Lett 2011;305:1-7.
25. Poeckel D, Greiner C, Verhoff M, Rau O, Tausch L, Hörnig C, et al. Carnosic acid and carnosol potently inhibit human 5-lipoxygenase and suppress pro-inflammatory responses of stimulated human polymorphonuclear leukocytes. Biochem Pharmacol 2008;76:91-7.