Türkiye'nin Doğu Bölgesinden Toplanan Bazı Yerel Ekmeklik Buğday Popülasyonlarından Seçilen Saf Hatların Yüksek Molekül Ağırlıklı Glutenin Alt Birimlerinin İncelenmesi

Bu araştırmada Türkiyenin doğu bölgesinden toplanan bazı yerel ekmeklik buğday popülasyonlarından seçilen saf hatlardan tane verimi, verim unsurları ve bazı kalite özelliklerine göre belirlenen 41 adet genotipin, SDS-PAGE yöntemi kullanılarak Glu-1 lokusunda bulunan yüksek molekül ağırlıklı glutenin (YMAG) alt birimleri incelenmiştir. Ekmeklik buğday saf hatlarında Glu-1 lokusuna ait 9 tane allel belirlenmiştir. Glu-A1 lokusuna ait sadece null alleli (c) belirlenirken, Glu-B1 lokusuna ait 7+8 alt birimi (b) 35 (% 85.4) saf hatta 13+16 alt birimi (f) 2 (% 4.9) saf hatta, 7 (a), 14+15 (h), 17+18 (i) ve 6+8 (d) alt birimleri ise sadece 1er (% 2.4) saf hatta gözlenmiştir. Glu-D1 lokusunda a alleli 34 (% 82.9) saf hatta, d alleli 7 (% 17.1) saf hatta belirlenmiştir. Yedi farklı YMAG alt birimleri kombinasyonlarının saptandığı çalışmada en yaygın olarak (% 78.0) gözlenen n, 7+8 ve 2+12 kombinasyonu 32 saf hatta belirlenmiştir. Sadece 5 saf hat (% 12.2) 8 kalite skor değerini alırken, 36 (% 87.8) saf hattın skor değerinin 4-6 arasında değiştiği tespit edilmiştir. Anahtar Kelimeler: Türkiye yerel ekmeklik buğdayları; SDS-PAGE; Yüksek molekül ağırlıklı glutenin (YMAG)

Analysis of High Molecular Weight Glutenin Subunit Composition of Pure Lines Selected from Eastern Turkey Bread Landraces

In this research, high molecular weight glutenin (HMWG) subunit composition, found on Glu-1 locus of 41 genotypes selected according to grain yield, yield components and some quality properties from bread wheat landraces collected from Eastern Turkey, has been investigated by using SDS-PAGE method. Of the bread wheat pure lines, nine Glu-1 locus alleles have been determined in genotypes. While only null allele (c) on Glu-A1 locus has been determined, on Glu-B1

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  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: Halit APAYDIN
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