Pegah RAMEZANI, Morteza SIAVOSHI, Anıl D. MORE, Mahmoubeh EBRAHIMI, Salman DASTAN

Mürdümük (Lathyrus sativus Linn.)’de Gama Işınımı ve EMS Kökenli Çiçek Rengi Mutasyonu

Pusa-24 mürdümük (Lathyrussativus Linn.) çeşidinde çiçek rengi mutantı oluşturmak için taze tohumlar fiziksel mutajen olarak 4 farklı dozda (100, 200, 300 ve 400 Gy) gama radyasyonu ve kimyasal mutajen olarak da 4 farklı dozda (5, 10, 15 ve 20 mM) EMS (etil metan sülfonat) ve bunların kombinasyonuna maruz bırakılmıştır. Mutagenesis nedeniyle M2 , M3 and M4 generasyonlarında geniş boyutta farklı çiçek rengi mutantları elde edilmiştir. Kontrol progenlerinde doğal olarak mavi olan çiçek rengine karşın değişik aynı ve farklı olan pembe, mor, beyaz ve sarı çiçek rankleri elde edilmiştir. En fazla çiçek rengi sıklığı 20 mM EMS uygulanmış tohumların 4’üncü generasyonlarında elde edilirken en düşük mutasyon sıklığı 100 Gy gama ışını uygulanmış tohumların 2’nci generasyonlarından elde edilmiştir. Bu araştırma sonuçları; her generasyonda yüksek çiçek rengi mutasyonunun yüksek doz/konsantrasyonlarda gerçekleştiğini göstermiştir.

Gamma Rays and EMS Induced Flower Color Mutation in Grasspea (Lathyrus sativus Linn.)

To induce flower color mutants in grasspea (Lathyrus sativus Linn.) variety of Pusa-24, the fresh seeds were exposed to 4 different doses of gamma radiation as physical mutagens (100, 200, 300 and 400 Gy) and treated with 4 different concentration of EMS (ethyl methanesulphonate) as chemical mutagen (5, 10, 15 and 20 mM) and also combination of both mutagens. Due to mutagenesis broad range of flower color mutants in M2 , M3 and M4 were obtained. Various unique and different flower color mutations like pink, purple, white and yellow detected against blue which is natural color in control progenies. Maximum flower color frequency was recorded in EMS treated seeds (20 mM) in 4th generation and lowest mutation frequency in 2nd generation was obtained in gamma rays (100 Gy) treated seeds. The results showed that more flower color mutations happened in more dose/concentration of mutagens in every generation.

PDF

___

Toker C, Uzun B, Canci H & Ceylan F O (2005). Effects of gamma irradiation on the shoot length of Cicer seeds. Radiation Physics and Chemistry 73: 365-367
Tripathy S K, Lenka D & Ranjan R (2011). Maximization of mutation of mutation frequency in grasspea (Lathyrus sativus L.). Legume Research 34: 296-299
Rathod K L (1989). Status of Lathyrus sativus Linn. in India with special reference to Madhya Pradesh. Third World Medical Research Foundation 11(1): 168-174
Palmer V S, Kaul A K & Spenser P S (1989). Proceeding of the international network for the improvement of Lathyrus sativus Linn. and the eradication of Lathyrism. Third World Medical Research Foundation 11(1): 219-223
Oluwatosin O B (2001). Inheritance of genes controlling flower color in cowpea (Vigna unguiculata L.Walp). Journal of Genetics and Breeding 1(4): 259-262
Mahamune S E & Kothekar V S (2011). Gamma ray induced flower color and seed mutants in French bean (Phaseolus vulgaris L.). Recent Research in Science and Technology 3(5): 33-35
Kaul A K, Islam M Q & Hamid A (1986). Screening of Lathyrus germplasm of Bangladesh for BOAA content and some agronomic characters. Third World Medical Research Foundation 11(1): 256-265
Girija M, Dhanavel D & Gnanamurthy S (2013). Gamma rays and EMS induced flower color and seed mutants in cowpea (Vigna unguiculata L. Walp). Advances in Applied Science Research 4(2): 134-139
Campbell C G, Mehra R B, Agrawal S K, Chen Y Z, Abdel A M, Moneim H I T, Kawaja C R, Yadav J U & Araya W A (1994). Current status and future strategy in breeding grasspea (Lathyrus sativus Linn.). Euphytica 73: 167-175
Campbell C G & Clayton G (1997). Grass pea (Lathyrus sativus Linn.) Promoting the conservation and use of underutilized and neglected crops. Institute of Plant Genetics and Crop Plant Research, Gatersleben/ International Plant Genetic Resources Institute, Rome, Italy
Borkar A T & More A D (2010). Induced flower color mutations in Phaseolus vulgaris Linn. through physical and chemical mutagens. Advances in Bioresearch 1(1): 22-28