Metin TUNA, Gülsemin SAVAŞ TUNA, Gülru YÜCEL, Gözde DUYU, Kardelen UZUN

Türkiye’nin Batı Bölgelerinden Toplanmış Hypericum perforatum L. Aksesyonlarının Çekirdek DNA İçerikleri ile Ploidi Düzeylerinin Belirlenmesi

Hypericum perforatum L. (sarı kantaron) depresyon ve kanser gibi rahatsızlıkların tedavisinde kullanılan, farmasötik açıdan önemli bileşikler üreten tıbbi bir bitkidir. H. perforatum, bitkilerin genetik yapısını ve kimyasal kompozisyonunu etkileyen birden fazla üreme mekanizması ile üreme yeteneğine sahip fakültatif apomiktik bir türdür. Bu çalışmanın amacı; Türkiye’nin doğal florasında bulunan H. perforatum L. aksesyonlarının çekirdek DNA içeriklerini ve ploidi düzeylerini belirlemektir. Çalışmada, Türkiye’nin 23 farklı lokasyonundan toplanan 39 Hypericum perforatum L. aksesyonuna ait tohumlar materyal olarak kullanılmıştır. Flow sitometri yöntemi ile H. perforatum aksesyonlarının herbiri için 3 farklı fide üzerinde çekirdek DNA analizi yapılmıştır. Flow sitometri analiz sonuçlarına göre aksesyonların çekirdek DNA içerikleri 0.8-2.57 pg 2C-1 arasında değişmiştir. Aksesyonların çekirdek DNA içerikleri arasındaki farklılıklar istatistiki açıdan (P

Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected from Western Turkey

Hypericum perforatum L. (St John’s Wort) is a medicinal plant that produces pharmaceutically important compounds with antidepressive and anticancer activities. H. perforatum is a facultative apomictic species as it has the ability to reproduce with multiple reproduction mechanisms affecting genetic structure and chemical composition of the plants. The objective of this study was to determine nuclear DNA contents and ploidy levels of H. perforatum L. plants growing naturally in the flora of Turkey. The seeds of 39 Hypericum perforatum L. accessions collected from 23 different locations in Turkey were used in the study. Nuclear DNA contents of three different seedlings for each of the 39 H. perforatum accessions were determined using flow cytometry. Based on the results of flow cytometric analysis, nuclear DNA contents of the accessions varied between 0.8-2.57 pg 2C-1. Nuclear DNA content differences observed among H. perforatum accessions were statistically significant (P

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