Bazı Orkide Türlerinin in vitro Simbiyotik Kültür Çalışmaları

Bu çalışmada Dactylorhiza iberica (Bieb. ex Willd.) Soó, Dactylorhiza umbrosa (Kar. et. Kir.) Soó ve Orchis palustris Jacquin türlerine ait in vitro kültüre alınmış tohumlardan protokorm ve sürgün oluşumları incelenmiştir. Simbiyotik kültürler, bitkilerin yumrularından izole edilen binükleik Rhizoctonia ve Rhizoctonia solani izolatları ile yulaf ortamı (YO) ve modifiye yulaf ortamından (MYO) oluşmaktadır. Protokorm ve sürgün oluşumunda en erken süre, sırasıyla, 42.67 gün ve 66 gün olarak YO’na ekilmiş olan D. umbrosa türünde belirlenmiştir. En yüksek protokorm gelişim ve en az kararan protokorm oluşum oranı sırasıyla % 60 oranında (YO) ve % 2.99 oranında (MYO) D. umbrosa türünden elde edilmiştir. Protokormlardan elde edilen sürgünler, en fazla YO’da gelişen D. iberica türünde % 35.04 olarak tespit edilmiştir. Tüm veriler, besi ortamlarında, binükleik Rhizoctonia izolatının kullanılması ile elde edilmiştir.

In vitro Symbiotic Culture Studies of Some Orchid Species

This study investigated the formation of protocorms and shoots from in vitro cultured seeds of Dactylorhiza iberica (Bieb. ex Willd.) Soó, D. umbrosa (Kar. and Kir.) Nevski, and Orchis palustris Jacquin. Culture conditions included binucleate Rhizoctonia and Rhizoctonia solani isolates, which were symbiotic cultures isolated from the tubers of these plants, and culture media consisting of an oat medium (OM) or a modified oat medium (MOM). The shortest times for protocorm and shoot development of D. umbrosa sowed in OM were 42.67 and 66 days, respectively. The highest rate of protocorm development and the lowest percentage of formation of darkened protocorms in D. umbrosa were 60% (in OM) and 2.99% (in MOM), respectively. The maximum percentage of shoots obtained from protocorms was 35.04% for D. iberica cultured in OM. All data were obtained using binucleate Rhizoctonia sp. inoculates in the nutrient media.

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