Dissection of Barley Landraces Originated From Twelve Different Countries by Using Simple Sequence Repeats Markers

Genetik çeşitliliğin önemli bir kaynağı olan yerel çeşitler, tahıl türlerinin geliştirilmesi için önemlidirler. Yerel çeşitler arasındaki genetik çeşitliliğin araştırılması, genetik kaynakları koruma ve arpa ıslahında gelecek stratejileri geliştirme için önemlidir. Bu çalışmada, on iki ülkeden köken alan arpa yerel çeşitlerindeki genetik çeşitlilik, basit sıra tekrarları (BST) işaretleyicileri kullanılarak çalışılmıştır. Yedi arpa bağlantı grubuna ait onaltı BST işaretleyicisi yüksek genetik çeşitliği ortaya çıkarmıştır. Toplamda 92 polimorfik allel sayılmış ve allel sayısı 5.75 ortalama ile lokus başına 1 ila 7 arasında olmuştur. Genetik çeşitlilik en yüksek Türkiye yerel çeşitlerinde (0.66 ± 0.13), en düşük Ukrayna yerel çeşitlerinde (0.38 ± 0.24) belirlenmiştir. Polimorfik lokus yüzdesi, Almanya, Holanda, Rusya, Türkiye ve Amerikada en yüksek yani % 100 iken, % 75 ile en düşük İngilterede bulunmuştur. Yerel çeşitlerin kümeleme analizleri onları dört ana gruba ayırmıştır. Sonuçlar gelecekteki ıslah çalışmaları için farklı ülkelerden arpa yerel çeşitleri hakkında ilave genetik bilgiler sağlamıştır. Anahtar Kelimeler: Arpa; Yerel çeşitler; BST; Çeşitlilik

Basit Sıra Tekrarı İşaretleyicilerini Kullanarak On İki Farklı Ülkeden Köken Alan Arpa Yerel Çeşitlerinin İncelenmesi

Landraces, as an important source of genetic diversity, are important for improvement of crop species. Investigating of genetic diversity among landraces is necessary to conserve genetic resources and develop future strategies on barley breeding. In this study, genetic diversity in barley landraces originating from twelve countries was studied using simple sequence repeat (SSR) markers. Sixteen SSR markers belong to the seven barley linkage groups revealed high genetic diversity. A total of 92 polymorphic alleles were scored and the number of alleles ranged from 1 to 7 per locus, with an average of 5.75. Genetic diversity was the highest in landraces from Turkey (0.66 ± 0.13) and the lowest in those from Ukraine (0.38 ± 0.24). While the highest percentage of polymorphic loci was found for landraces from Germany, Netherland, Russia, Turkey and USA as 100%, the lowest one was in England with 75%. Clustering analysis of landraces divided them into four main groups. The results provided additional genetic information about the barley landraces from different countries for future breeding process.

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  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: Halit APAYDIN
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