Periimplantal Kemik Defektlerinin Titanyum Tüplerde Hazırlanan Trombositten Zengin Fibrin ile Kemik Rejenerasyonunun Histomorfometrik Değerlendirmesi: Tavşan Tibiasinda Deneysel Çalışma

Amaç: Bu çalışmanın amacı titanyum tüplerde hazırlanan trombositten zengin fibrinin (T-PRF) deneysel olarak hazırlanmış periimplantal kemik defektlerindeki rejenerasyonu incelemektir. Gereç ve Yöntem: Çalışmada 12 adet erkek wistar rat kullanılmış olup her bir tavşanın sağ ve sol tibiasına trephan frez ile 7 mm genişliğinde 4 mm derinliğinde defektler oluşturulmuştur. Daha sonra 3,3X8 mm lik implantlar tibialara yerleştirilmiş olup periimplantal defektler sırasıyla Otojen greft, T-PRF, Bifazik Kalsiyum Fosfat greft (BCP) ile restore edilmiştir. Kontrol grubu boş bırakılıp spontan iyileşmeye bırakılmıştır. 4 hafta sonra tüm tavşanlarötenazi ile öldürülerek implant ile beraber etrafındaki kemik alındı. Kemik-implant kontağı toludin mavisi ile boyanan kesitlerin histomorfometrik incelenmesi ile değerlendirilmiştir. Bulgular: Otojen grupta (5267,50±228,95) kemik implant kontağı değeri en yüksek çıkmıştır. Ancak otojen grup ile T-PRF grubu (3932,50±275,50) arasında istatistiksel olarak bir fark gözlenmemiştir. BCP grubu ile kontrol grubunda en düşük BIC değerleri gözlenmiştir. Sonuçlar: Peri-implantal defektlerin rejenerasyonunda T-PRF grubunda neredeyse otojen grup kadar BIC değerleri bulunmuştur. Bu yüzden T-PRF gelecekte yönlendirilmiş kemik rejenerasyonunda otojen matriksfonksiyonu görevi görebilir.

Histomorphometric Evaluation of Bone Regeneration in Peri-Implant Osseous Defects Treated With Titanium Prepared Platelet Rich Fibrin: An Experimental Study in a Rabbit Model

Aim: The present study aims to investigate the healing of artificially createdperi-implant osseous defects using Titanium-Platelet Rich Fibrin Materialand Method: Bone defects (9-mm diameter, 4-mm depth) were created andimplant beds (3-mm diameter, 6-mm depth) were prepared in the middle ofthem in rabbit tibias (24 rabbits) used as the experimental model. Afterwards,dental implants were installed into the left and right tibia (diameter 3.0 mm,length 8 mm). In the experimental groups, the peri-implant defect was filledwith Autogenous grafts (AG), Titanium-Platelet Rich Fibrin (T-PRF), BiphasicCalcium Phosphate grafts (BCP). The control group did not receive any filling.The bone-to-implant contact (BIC) was obtained by histomorphometrically examining the sections taken from the study groups.Results: AG group was resulted in a high degreeof BIC. No difference of statistical significance wasfound for the BIC as far as autogenous graft and PRFgroups are concerned. Conclusion: Peri-implantaldefects in regeneration of T-PRF were found to benearly as affective as the autogenous bone graft.We, therefore, conclude that T-PRF can be used forguided bone regeneration in the function of autogenicmatrix in the future.

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