Muhammet Yusuf TEPEBAŞI, Pınar ASLAN KOŞAR, Okan SANCER

Escherichia coli’nin Üriner Sistem Epitelyal Hücrelerinde Mikronükleus Testi ile Genotoksik Etkisinin İncelenmesi

Object: E. coli is a type of bacterium that causes urinary tract infections in women. The aim of the work was to investigate the possible use of epithelial cells from the urinary tract in identifying infections–related DNA damage effects in women. Methods: Epithelial cells from urinary tract were collected from 40 E .coli infection and 40 control (non- infection) women and subjected to micronucleus assay. Number of cells with micronucleated, binucleated cells were counted in two groups as parameters for the evaluation of genome stability. All of urine samples pH, leukocyte, leukocyte esterase, nitrite results measured with H-800 urine analyzer and FUS-200 urine sediment analyzer. Result: E. coli infection and non-infection groups test results were statistically evaluated with Mann-Whitney U and Independent Sample T test. A p-value of 0,05). Conclusion: The detection of E. coli in urinary tract epithelial cells showed that induce DNA damage. It can be said that patients with early antibiotics and doses, early treatment may help to reduce the level of DNA damage

Investigation of Genotoxic Effect of Escherichia coli in Urinary Tract Epithelial Cells with Micronucleus Assay

Amaç: E. coli, kadınlarda idrar yolu enfeksiyonlarına neden olan bir bakteritürüdür. Bu çalışmada kadınların idrar yolu epitel hücrelerinde, enfeksiyonabağlı DNA hasar etkilerinin araştırılması amaçlandı. Metot: 40 E. coli enfeksiyonuolan ve 40 enfeksiyon olmayan kadınların idrar yolu epitel hücreleri,toplandı ve mikronükleus analizine tabi tutuldu. Her iki grupta mikronükleusve binükleus parametreleri genom stabilitesinin değerlendirilmesi için kullanıldı.Tüm idrar örneklerinin pH, lökosit, lökosit esteraz, nitrit değerleri H-800ve FUS-200 tam otomatik idrar cihazı ile analiz edildi. Bulgular: E. coli enfeksiyonuolan ve enfeksiyon olmayan grupların sonuçları istatistiksel olarakMann-Whitney U ve Independent Sample T testi ile değerlendirildi. Mikronükleus,binukleus, idrar, lökosit, lökosit esteraz ve nitrit parametreleri için sonuçlaristatistiksel olarak anlamlı bulundu (p0,05).Sonuç: İdrar yolu epitel hücrelerinde bulunan E. coli’ nin, DNA hasarını indüklediğigörülmüştür. Antibiyotik tedavisinin hastalarda erken kullanımı veuygun dozda kullanımı ile DNA hasar seviyesini azaltmaya yardımcı olabileceğisöylenebilir.

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