An assesment of domoic acid levels as a function of salinity-temperature variations in the Sea of Marmara

This study presents the domoic acid (DA), responsible from Amnesic Shellfish Poisoning (ASP), levels of plankton net samples in the Sea of Marmara during April, July and October 2019. Plankton net samples were collected from 13 stations, which were located close to the Istanbul metropolitan area, and along the east and southwest parts of the Sea of Marmara. DA measurements were done by high-performance liquid chromatography (HPLC), using the diode-array detection (detection limit (LOD): 0.108 µg mL−1). Twenty-two out of the 26 plankton net samples (84.6%) were found to contain DA during the study period and the concentrations varied between 0.22 and 3.45 µg mL−1. Possible relationships between physical parameters (salinitytemperature) and DA concentrations were investigated. DA production was mainly controlled by salinity (r:0.47, p

Marmara Denizi’ndeki domoik asit seviyelerinin tuzluluk-sıcaklık değişimlerinin bir fonksiyonu olarak değerlendirilmesi

Bu çalışmada Nisan, Temmuz ve Ekim 2019 dönemlerinde Marmara Denizi’nden alınan plankton kepçe örneklerinde Amnezik Kabuklu Zehirlenmesi’ne (AKZ) neden olan denizel biyotoksin, domoik asit (DA), seviyeleri sunulmaktadır. Plankton kepçe örneklemeleri, Marmara Denizi’nin doğusunda, güneybatısında ve İstanbul metropol bölgesinin yakınında bulunan toplam 13 adet istasyondan yapılmıştır. Domoik asit ölçümleri, diode-array dedeksiyonu kullanılarak Yüksek Performanslı Sıvı Kromatografisi yöntemiyle gerçekleştirilmiştir (Belirleme limiti: 0,108 µg mL−1). Çalışma dönemi boyunca alınan toplam 26 adet plankton kepçe örneğinden 22’sinin (%84,6) DA içerdiği tespit edilmiş ve bu örneklerdeki konsantrasyon aralığı 0,22 – 3,45 µg mL−1 olarak belirlenmiştir. DA konsantrasyonları ile fiziksel parametreler (tuzluluk ve sıcaklık) arasındaki olası ilişkiler incelenmiş ve çalışma dönemi boyunca DA üretiminin genel olarak tuzluluk (r:0,47, p

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Aktan, Y., Tüfekçi, V., Tüfekçi, H. & Aykulu, G. (2005). Distribution patterns, biomass estimates and diversity of phytoplankton in Izmit Bay (Turkey). Estuarine, Coastal and Shelf Science, 64(2–3), 372–384. DOI:10.1016/j.ecss.2005.03.003

Almandoz, G. O., Ferreyra, G.A., Schloss, I.R., Dogliotti, A.I., Rupolo, V., Paparazzo, F.E., & Ferrario, M.E. (2008). Distribution and ecology of Pseudo-nitzschia species (Bacillariophyceae) in surface waters of the Weddell Sea (Antarctica). Polar Biology, 31(4), 429–442. DOI:10.1007/s00300-007-0369-9

Bates, S., Freitas, A.S.W. de, Milley, J.E., Pocklington, R., Quilliam, M.A., Smith, J.C. & Worms, J. (1991). Controls on Domoic Acid Production by the Diatom Nitzschia pungens f. multiseries in Culture: Nutrients and Irradïance. Canadian Journal of Fisheries and Aquatic Sciences, 48(7), 1136–1144. DOI:10.1139/f91-137

Bates, S., Hubbard, K.A., Lundholm, N., Montresor, M. & Leaw, C.P. (2018). Pseudo-nitzschia, Nitzschia, and domoic acid: New research since 2011. Harmful Algae, 79, 3–43. DOI:10.1016/j.hal.2018.06.001

Beşiktepe, Ş., Sur, H.I., Özsoy, E., Latif, M.A., Oǧuz, T. & Ünlüata, Ü. (1994). The circulation and hydrography of the Marmara Sea. Progress in Oceanography, 34(4), 285-334. DOI:10.1016/0079-6611(94)90018-3

Buck, K.R. (1992). Autecology of the diatom Pseudonitzschia australis, a domoic acid producer, from Monterey Bay, California. Marine Ecology Progress Series, 84(3), 293–302. DOI:10.3354/meps084293

Busse, L.B., Venrick, E.L., Antrobus, R., Miller, P.E., Vigilant, V., Silver, M.W., & Prezelin, B.B. (2006). Domoic acid in phytoplankton and fish in San Diego, CA, USA. Harmful Algae, 5(1), 91–101. DOI:10.1016/j.hal.2005.06.005

Dao, H.V., Takata, Y., Omura, T., Sato, S., Fukuyo, Y. & Kodama, M. (2009). Seasonal variation of domoic acid in a bivalve Spondylus versicolor in association with that in plankton samples in Nha Phu Bay, Khanh Hoa, Vietnam. Fisheries Science, 75(2), 507–512. DOI:10.1007/s12562-009-0057-5

Deniz, N. & Tas, S. (2009). Seasonal variations in the phytoplankton community in the north-eastern sea of marmara and a species list. Journal of the Marine Biological Association of the United Kingdom, 89(2), 269–276. DOI:10.1017/S0025315409003117

Dursun, F., Yurdun, T. & Ünlü, S. (2016). The First Observation of Domoic Acid in Plankton Net Samples from the Sea of Marmara, Turkey. Bulletin of Environmental Contamination and Toxicology, 96(1), 70–75. DOI:10.1007/s00128-015-1704-4

Dursun, F., Ünlü, S., Tas, S. & Yurdun, T. (2017). Domoic acid variations in response to environmental conditions in an eutrophic estuary, Golden Horn (Turkey). Turkish Journal of Fisheries and Aquatic Sciences, 17(4), 653–662. DOI:10.4194/1303-2712-v17_4_01

Dursun, F., Ünlü, S. & Yurdun, T. (2018). Determination of Domoic Acid in Plankton Net Samples from Golden Horn Estuary, Turkey, Using HPLC with Fluorescence Detection. Bulletin of Environmental Contamination and Toxicology, 100(3), 457–462. DOI:10.1007/s00128-018-2275-y

Dursun, F. & Tas, S. (2019). Variations in abundance and diversity of phytoplankton in the surface waters of the Golden Horn Estuary (Sea of Marmara). Journal of the Marine Biological Association of the United Kingdom, 99(2), 279–290. DOI:10.1017/S0025315418000073

Fire, S.E., Wang, Z., Berman, M., Langlois, G.W., Morton, S.L., SekulaWood, E. & Benitez-Nelson, C. R. (2010). Trophic transfer of the harmful algal toxin domoic acid as a cause of death in a minke whale (Balaenoptera acutorostrata) stranding in southern California. Aquatic Mammals, 36(4), 342–350. DOI:10.1578/AM.36.4.2010.342

Granéli, E. & Flynn, K. (2006). Chemical and Physical Factors Influencing Toxin Content. In Ecology of Harmful Algae (pp. 229–241). DOI:10.1007/978-3-540-32210-8_18

Hallegraeff, G.M. (2004). Harmful algal blooms: a global overview. Manual on Harmful Marine Microalgae, 33, 1-22.

Hasle, G.R. (2002). Are most of the domoic acid-producing species of the diatom genus Pseudo-nitzschia cosmopolites? Harmful Algae, 1(2), 137–146. DOI:10.1016/S1568-9883(02)00014-8

Klein, C., Claquin, P., Bouchart, V., Le Roy, B. & Véron, B. (2010). Dynamics of Pseudo-nitzschia spp. and domoic acid production in a macrotidal ecosystem of the Eastern English Channel (Normandy, France). Harmful Algae, 9(2), 218–226. DOI:10.1016/j.hal.2009.10.004

Kudela, R., Roberts, a, & Armstrong, M. (2004). Laboratory analyses of nutrient stress and toxin accumulation in Pseudo-nitzschia species from Monterey Bay, California. Harmful Algae 2002, 136–138.

Lapworth, C., Hallegraeff, G.M.J., & Ajani, P.A. (2001). Identification of domoic-acid-producing Pseudo-nitzschia species in Australian waters. In Harmful Algal Blooms 2000 Proceedings of the Ninth International Conference on Harmful Algal Blooms (pp. 38–41).

Lefebvre, K.A. & Robertson, A. (2010). Domoic acid and human exposure risks: A review. Toxicon, 56(2), 218-230 DOI:10.1016/j.toxicon.2009.05.034

Lewis, N., Bates, S., Mclachlan, J. & Smith, J. (1993). Temperature effects on growth, domoic acid production, and morphology of the diatom Nitzschia pungens f. multiseries. In: Toxic phytoplankton blooms in the sea (Ed. by T.J. Smayda & Y. Shimizu). In Elsevier Science Publishers B.V (pp. 601–606).

Likumahua, S., de Boer, M.K., Krock, B., Nieuwenhuizen, T., Tatipatta, W.M., Hehakaya, S., & Buma, A.G.J. (2019). First record of the dynamics of domoic acid producing Pseudo-nitzschia spp. in Indonesian waters as a function of environmental variability. Harmful Algae, 90. DOI:10.1016/j.hal.2019.101708

Ljubešić, Z., Bosak, S., Viličić, D., Borojević, K.K., Marić, D., Godrijan, J., & Dakovac, T. (2011). Ecology and taxonomy of potentially toxic Pseudonitzschia species in Lim Bay (north-eastern Adriatic Sea). Harmful Algae, 10(6), 713–722. DOI:10.1016/j.hal.2011.06.002

Lundholm, N., Hansen, P.J., & Kotaki, Y. (2004). Effect of pH on growth and domoic acid production by potentially toxic diatoms of the genera Pseudo-nitzschia and Nitzschia. Marine Ecology Progress Series, 273, 1–15. DOI:10.3354/meps273001

Macintyre, H.L., Stutes, A.L., Smith, W.L., Dorsey, C.P., Annabraham, & Dickey, R.W. (2011). Environmental correlates of community composition and toxicity during a bloom of Pseudo-nitzschia spp. in the northern Gulf of Mexico. Journal of Plankton Research, 33(2), 273–295. DOI:10.1093/plankt/fbq146

Milley, J.E., Bates, S., Bird, C.J., De Freitas, A.S.W. & Quilliamt, M.A. (1990). Trace determination of domoic acid in seawater and phytoplankton by high-performance liquid chromatography of the fluorenylmethoxycarbonyl (fmoc) derivative. International Journal of Environmental Analytical Chemistry, 38(3), 351–368. DOI:10.1080/03067319008026940

Perl, T.M., Bédard, L., Kosatsky, T., Hockin, J.C., Todd, E.C.D. & Remis, R.S. (1990). An Outbreak of Toxic Encephalopathy Caused by Eating Mussels Contaminated with Domoic Acid. New England Journal of Medicine, 322(25), 1775–1780. DOI:10.1056/nejm199006213222504

Saeed, A.F., Awan, S.A., Ling, S., Wang, R. & Wang, S. (2017). Domoic acid: Attributes, exposure risks, innovative detection techniques and therapeutics. Algal Research, 24, 97-110. DOI:10.1016/j.algal.2017.02.007

Sahraoui, I., Grami, B., Bates, S., Bouchouicha, D., Chikhaoui, M.A., Mabrouk, H.H. & Hlaili, A. S. (2012). Response of potentially toxic Pseudo-nitzschia (Bacillariophyceae) populations and domoic acid to environmental conditions in a eutrophied, SW Mediterranean coastal lagoon (Tunisia). Estuarine, Coastal and Shelf Science, 102–103, 95– 104. DOI:10.1016/j.ecss.2012.03.018

Schnetzer, A., Miller, P.E., Schaffner, R.A., Stauffer, B.A., Jones, B.H., Weisberg, S.B., … Caron, D.A. (2007). Blooms of Pseudo-nitzschia and domoic acid in the San Pedro Channel and Los Angeles harbor areas of the Southern California Bight, 2003-2004. Harmful Algae, 6(3), 372–387. DOI:10.1016/j.hal.2006.11.004

Scholin, C.A., Gulland, F., Doucette, G.J., Benson, S., Busman, M., Chavez, F.P., & Van Dolah, F.M. (2000). Mortality of sea lions along the central California coast linked to a toxic diatom bloom. Nature, 403(6765), 80– 84. DOI:10.1038/47481

Takahashi, E., Yu, Q., Eaglesham, G., Connell, D.W., McBroom, J., Costanzo, S., & Shaw, G. R. (2007). Occurrence and seasonal variations of algal toxins in water, phytoplankton and shellfish from North Stradbroke Island, Queensland, Australia. Marine Environmental Research, 64(4), 429–442. DOI:10.1016/j.marenvres.2007.03.005

Takemoto, T. & Daigo, K. (1958). Constituents of Chondria armata. Chemical and Pharmaceutical Bulletin, 6(5), 578–580. DOI:10.1248/cpb.6.578b

Taş, S., Okuş, E., Ünlü, S. & Altiok, H. (2011). A study on phytoplankton following Volgoneft-248 oil spill on the north-eastern coast of the Sea of Marmara. Journal of the Marine Biological Association of the United Kingdom, 91(3), 715–725. DOI:10.1017/S0025315410000330

Tas, S. & Yilmaz, I.N. (2015). Potentially harmful microalgae and algal blooms in a eutrophic estuary in the Sea of Marmara (Turkey). Mediterranean Marine Science, 16(2), 432–443. DOI:10.12681/mms.1042

Tas, S., Dursun, F., Aksu, A. & Balkis, N. (2016). Presence of the diatom genus Pseudo-nitzschia and particulate domoic acid in the Golden Horn Estuary (Sea of Marmara, Turkey). Diatom Research, 31(4), 339–349. DOI:10.1080/0269249X.2016.1247020

Tas, S. & Lundholm, N. (2017). Temporal and spatial variability of the potentially toxic Pseudo-nitzschia spp. in a eutrophic estuary (Sea of Marmara). Journal of the Marine Biological Association of the United Kingdom, 97(7), 1483–1494. DOI:10.1017/S0025315416000837

Teng, S.T., Lim, H.C., Lim, P.T., Dao, V.H., Bates, S. & Leaw, C. P. (2014). Pseudo-nitzschia kodamae sp. nov. (Bacillariophyceae), a toxigenic species from the strait of Malacca, Malaysia. Harmful Algae, 34, 17–28. DOI:10.1016/j.hal.2014.02.005

Tenorio, C., Uribe, E., Gil-Kodaka, P., Blanco, J. & Álvarez, G. (2016). Morphological and toxicological studies of Pseudo-nitzschia species from the central coast of Peru. Diatom Research, 31(4), 331–338. DOI:10.1080/0269249X.2016.1247018

Thessen, A.E., Dortch, Q., Parsons, M.L. & Morrison, W. (2005). Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. Journal of Phycology, 41(1), 21–29. DOI:10.1111/j.1529-8817.2005.04077.x

Trainer, V.L., Adams, N.G., Bill, B.D., Stehr, C.M., Wekell, J.C., Moeller, P., & Woodruff, D. (2000). Domoic acid production near California coastal upwelling zones, June 1998. Limnology and Oceanography, 45(8), 1818–1833. DOI:10.4319/lo.2000.45.8.1818

Trainer, V.L., Bates, S., Lundholm, N., Thessen, A.E., Cochlan, W.P., Adams, N.G. & Trick, C. G. (2012). Pseudo-nitzschia physiological ecology, phylogeny, toxicity, monitoring and impacts on ecosystem health. Harmful Algae, 14, 271–300. DOI:10.1016/j.hal.2011.10.025

Ünlülata, Ü., Oğuz, T., Latif, M.A. & Özsoy, E. (1990). On the Physical Oceanography of the Turkish Straits. In The Physical Oceanography of Sea Straits (pp. 25–60). DOI:10.1007/978-94-009-0677-8_2

Vale, P. & Sampayo, M.A.M. (2001). Domoic acid in Portuguese shellfish and fish. Toxicon, 39(6), 893–904. DOI:10.1016/S0041-0101(00)00229-4

Vrieling, E.G., Gieskes, W.W.C., Vrieling, E.G., Veenhuis, M., Koeman, R.P.T., Scholin, C. A., & Peperzak, L. (1996). Identification of a domoic acid-producing pseudo-nitzschia species (bacillariophyceae) in the dutch wadden sea with electron microscopy and molecular probes. European Journal of Phycology, 31(4), 333–340. DOI:10.1080/09670269600651561