Ali Doğan DURSUN, Hakan FIÇICILAR, Metin BAŞTUĞ, Demet TEKİN

KISA DÖNEM ANTRENMANIN İSKELET KASINDA KAVEOLİN VE VEGF EKSPRESYONU ÜZERİNE ETKİSİ.

Dayanıklılık egzersizi iskelet kası ve kardiyovasküler sistemde adaptif değişikliklere yol açmaktadır. Egzersiz anjiyogenezin önemli bir mediatörü olan vasküler endotelyal büyüme faktörünü (VEGF) indüklemektedir. Kaveolin-1 ve -3, kaveoller olarak bilinen hücre membran oluşumlarını oluşturan protein ünitelerdir ve kaveolin-reseptör-postreseptör an active part in the signal transduction regulation etkileşimleri yoluyla sinyal iletiminin regülasyonunda aktif rol alırlar. Kaveolin-1’in VEGF ile indüklenen sinyal kaskadında yer aldığı gösterilmiştir. induced signal cascades. The aim of the study was to Çalışmada kısa dönem egzersiz antrenmanının farklı investigate the effect of short term training on mRNA lif kompozisyonlarından oluşan iskelet kaslarında VEGF ve kaveolin-1 ve -3 mRNA düzeyleri üzerindeki etkisinin incelenmesi amaçlanmıştır. Çalışmada 3-4 months aged Wistar Albino rats were accustomed 3-4 aylık Wistar Albino türü erkek sıçanlara 10 gün süreyle koşu bandına ve egzersize alıştırma programı uygulandı. Takiben denekler alıştırma (n=6) ve antrenman grubu (n=6) olarak ikiye ayrıldı. Antrenman grubu ratlara 3 günlük kısa dönem dayanıklılık antrenmanı yaptırıldı (20-25 metre/dakika, %10 eğim, 85 dakika/gün). Koşu bandı deneylerine katılmayan ratlardan üçüncü deney grubu olarak kontrol grubu oluşturuldu (n=6). Deneklerin gastroknemius (kırmızı ve beyaz kısımları), plantaris ve soleus kas örneklerinden elde edilen total RNA’larından ters-trankripsiyon PCR ile cDNA sentezlendi. VEGF164, VEGF188, kaveolin-1, kaveolin-3 ve GAPDH PCR amplifikasyonunu takiben agaroz jel elektroforezi ve ardından UV kamera görüntülerinden bant yoğunluk analizleri yapıldı. Antrenman grubunda kontrol ve alıştırma gruplarına göre gastroknemius kası kırmızı kısımda VEGF164 mRNA düzeyinde artış olduğu gösterilmiştir (Kruskal Wallis Test: p=0.033, posthoc Tukey HSD: kontrol vs antrenman p=0.014, kontrol vs alıştırma p=0.995, alıştırma vs antrenman p=0.016). Kaveolin-1 ve -3 düzeylerinde değişiklik saptanmamıştır. VEGF164 mRNA’sındaki artış anjiyogenik mekanizmaların tetiklendiğine işaret etmektedir. Anjiyogenik mekanizmalarda kaveolin-VEGF etkileşiminin anlaşılabilmesi için ek çalışmalar gerekmektedir

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KISA DÖNEM ANTRENMANIN İSKELET KASINDA KAVEOLİN VE VEGF EKSPRESYONU ÜZERİNE ETKİSİ.

Endurance training leads to adaptational changes in skeletal muscle and cardiovascular system. Exercise induces vascular endothelial growth factor; one of the important mediator of angiogenesis. Caveolin-1 and -3 are the protein units forming the cell membrane formations as called the caveolae and take uygulandı. Takiben denekler alıştırma (n=6) ve antrenman grubu (n=6) olarak ikiye ayrıldı. Antrenman grubu ratlara 3 günlük kısa dönem dayanıklılık antrenmanı yaptırıldı (20-25 metre/dakika, %10 eğim, 85 dakika/gün). Koşu bandı deneylerine katılmayan ratlardan üçüncü deney grubu olarak kontrol grubu oluşturuldu (n=6). Deneklerin gastroknemius (kırmızı ve beyaz kısımları), plantaris ve soleus kas örneklerinden elde edilen total RNA’larından ters-trankripsiyon PCR ile cDNA sentezlendi. VEGF164, VEGF188, kaveolin-1, kaveolin-3 ve GAPDH PCR amplifikasyonunu takiben agaroz jel elektroforezi ve ardından UV kamera görüntülerinden bant yoğunluk analizleri yapıldı. Antrenman grubunda kontrol ve alıştırma gruplarına göre gastroknemius kası kırmızı kısımda VEGF164 mRNA düzeyinde artış olduğu gösterilmiştir (Kruskal Wallis Test: p=0.033, posthoc Tukey HSD: kontrol vs antrenman p=0.014, kontrol vs alıştırma p=0.995, alıştırma vs antrenman p=0.016). Kaveolin-1 ve -3 düzeylerinde değişiklik saptanmamıştır. VEGF164 mRNA’sındaki artış anjiyogenik mekanizmaların tetiklendiğine işaret etmektedir. Anjiyogenik mekanizmalarda kaveolin-VEGF etkileşiminin anlaşılabilmesi için ek çalışmalar gerekmektedir. into two groups as adaptation group (n=6) and training group (n=6). Training group rats underwent a 3 days short term endurance training program (20-25 meter/minute, %10 inclination, 85 minute/day). A control group was formed as a third experimental group from the rats that did not participate in any of the treadmill experiments (n=6). The total RNA obtained from the gastrocnemius (red and white portions), plantaris and soleus muscles, was used to synthesize cDNA via reverse transcription PCR method. Following VEGF164, VEGF188, caveolin-1, caveolin-3 and GAPDH PCR amplifications agarose gel electrophoresis and band density analysis of UV camera images were performed. VEGF164 mRNA levels of gastrocnemius muscle red portion were induced in training group comparing to the control and adaptation groups (Kruskal Wallis Test: p=0.033, posthoc Tukey HSD: control vs training p=0.014, adaptation vs training p=0.016, control vs adaptation p=0.995). Caveolin-1 and caveolin-3 levels were unchanged. The increase of VEGF164 mRNA levels indicates that the angiogenic mechanisms were triggered. In order to understand the interaction of caveolin-VEGF in angiogenic mechanisms, additional studies are needed

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