Ali Doğan DURSUN, Metin BAŞTUĞ, Hakan FIÇICILAR, Demet TEKİN

Kısa dönem antrenmanın iskelet kasında kaveolin ve VEGF ekspresyonu üzerine etkisi

Dayanıklılık egzersizi iskelet kası ve kardiyovasküler sistemde adaptif değişikliklere yol açmaktadır. Egzersiz anjiyogenezin önemli bir mediatörüolan vasküler endotelyal büyüme faktörünü (VEGF)indüklemektedir. Kaveolin-1 ve -3, kaveoller olarakbilinen hücre membran oluşumlarını oluşturan protein ünitelerdir ve kaveolin-reseptör-postreseptöretkileşimleri yoluyla sinyal iletiminin regülasyonunda aktif rol alırlar. Kaveolin-1in VEGF ile indüklenen sinyal kaskadında yer aldığı gösterilmiştir.Çalışmada kısa dönem egzersiz antrenmanının farklılif kompozisyonlarından oluşan iskelet kaslarındaVEGF ve kaveolin-1 ve -3 mRNA düzeyleri üzerindekietkisinin incelenmesi amaçlanmıştır. Çalışmada3-4 aylık Wistar Albino türü erkek sıçanlara 10 günsüreyle koşu bandına ve egzersize alıştırma programı uygulandı. Takiben denekler alıştırma (n=6) ve antrenman grubu (n=6) olarak ikiye ayrıldı. Antrenman gruburatlara 3 günlük kısa dönem dayanıklılık antrenmanıyaptırıldı (20-25 metre/dakika, %10 eğim, 85 dakika/ gün). Koşu bandı deneylerine katılmayan ratlardanüçüncü deney grubu olarak kontrol grubu oluşturuldu(n=6). Deneklerin gastroknemius (kırmızı ve beyaz kı- sımları), plantaris ve soleus kas örneklerinden elde edilen total RNAlarından ters-trankripsiyon PCR ile cDNAsentezlendi.$VEGF_{164} ,VEGF_{188'}$ kaveolin-1, kaveolin-3 veGAPDH PCR amplifikasyonunu takiben agaroz jel elektroforezi ve ardından UV kamera görüntülerinden bantyoğunluk analizleri yapıldı. Antrenman grubunda kontrol ve alıştırma gruplarına göre gastroknemius kasıkırmızı kısımda $VEGF_{164}$ mRNA düzeyinde artış olduğugösterilmiştir (Kruskal Wallis Test: p=0.033, posthocTukey HSD: kontrol vs antrenman p=0.014, kontrol vsalıştırma p=0.995, alıştırma vs antrenman p=0.016).Kaveolin-1 ve -3 düzeylerinde değişiklik saptanmamıştır.$VEGF_{164}$mRNAsındaki artış anjiyogenik mekanizmaların tetiklendiğine işaret etmektedir. Anjiyogenik mekanizmalarda kaveolin-VEGF etkileşiminin anlaşılabilmesiiçin ek çalışmalar gerekmektedir.

Effect of short term training on caveolin and VEGF expressions in skeletal muscle

Endurance training leads to adaptational changesin skeletal muscle and cardiovascular system.Exercise induces vascular endothelial growth factor;one of the important mediator of angiogenesis.Caveolin-1 and -3 are the protein units forming the cellmembrane formations as called the caveolae and takean active part in the signal transduction regulationby caveolin-receptor-postreseptor interactions. Ithas been shown that caveolin-1 takes place in VEGFinduced signal cascades. The aim of the study was toinvestigate the effect of short term training on mRNAlevels of VEGF and caveolin-1 and -3 in the skeletalmuscles composed of different fiber compositions.3-4 months aged Wistar Albino rats were accustomedto the treadmill and exercise within a 10 days ofadaptation training program. Then rats were dividedgroup (n=6). Training group rats underwent a 3 daysshort term endurance training program (20-25 meter/ minute, %10 inclination, 85 minute/day). A controlgroup was formed as a third experimental groupfrom the rats that did not participate in any of thetreadmill experiments (n=6). The total RNA obtainedfrom the gastrocnemius (red and white portions),plantaris and soleus muscles, was used to synthesizecDNA via reverse transcription PCR method. Following$VEGF_{164} ,VEGF_{188'}$ caveolin-1, caveolin-3 and GAPDH PCRamplifications agarose gel electrophoresis and banddensity analysis of UV camera images were performed.$VEGF_{164}$ mRNA levels of gastrocnemius muscle redportion were induced in training group comparing tothe control and adaptation groups (Kruskal WallisTest: p=0.033, posthoc Tukey HSD: control vs trainingp=0.014, adaptation vs training p=0.016, control vsadaptation p=0.995). Caveolin-1 and caveolin-3 levelswere unchanged. The increase of $VEGF_{164}$mRNAlevels indicates that the angiogenic mechanisms weretriggered. In order to understand the interaction ofcaveolin-VEGF in angiogenic mechanisms, additionalstudies are needed.

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