Injecting the prepubertal laboratory mice with a mixture of serine and threonine and its effects on spermatogenic function

Bu çalışmanın amacı; farelerde Serine ve Threonine amino asitlerinin birlikte enjekte edilmesinin spermatogenik fonk-siyonlar üzerine etkisini araştırmaktır. Bu amaçla prepubertal laboratuar faresine (QS türü) 5 gün süre ile Salına (Kontrol) veya Serine ile Threonine amino asit karışımı (Test) enjekte edildi. Son enjeksiyonu takiben teslisler alındı ve deklârasyona tabi tutuldu. Tesetislerden kesitler alınarak PAS (Periodic Acid ve Schiff) boyası ile bayandı. Boyanan kesiler üzerindeki Leydig hücrelerinin, tubulus Seminiferous contortusların çapları ölçüldü ve aynı anda her kesit üzerinde yuvarlak sper-matid sayısı tespit edildi. Alınan sonuçlara göre Serine ve Threonine enjeksiyonu; tubulus contortusların ve Leydig hücrel-erinin çaplarında bir değişikliğe neden olmadı fakat yuvarlak spermatidlerin sayısını azalttı (P

Anahtar Kelimeler:

treonin, enjeksiyon, leydig hücreleri, dönüştürücü büyüme etkeni, spermatozoa, fareler, serin, amino asitler, sperma oluşması, testis

Serine ve threonine amino asitlerinin prepubertal labaratuar faresine enjekte edilmesinin spermatogenik

The aim of this study is to determine the effect of injecting a mixture of Serine and Threonine on spermatogenic function within testes of prepubertal mice (QS strain). Therefore, the mice were injected with Saline (Control) or a mixture of Serine and Threonine (Test) for 5 days. Following the last injection, the testes were removed and dehydrated then stained with PAS (Periodic Acid Schiff). The diameter of tubules, diameter ofLeydig cells and the numbers of round spermatids were measured on sections of the testes. The number of stage round spermatids was decreased by Serine and Threonine injection (P<0.05). The total numbers of round spermatids per testis for control and test groups were detected as 1.66+0.32 and 1.06+0.05 $(x10^6/mm3)$ respectively. This experiment shows that Serine and Threonine injection reduced the number of round spermatids while the diameters ofLeydig cells and the diameters of tubules were not affected. The reason for reduced number of round spermatids might be a result of increased expression of TGF- $\beta$ which might increase the apoptosis of the spermatogonia and this probably resulted with the reduction in the number of round spermatids.

Keywords:

threonine, injection, Leydig cells, transforming growth factor, spermatozoa, mice, serine, amino acids, spermatogenesis, testes,

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