Fibronilin soğan (Allium cepa L.) kök ucu hücrelerine sitogenetik etkileri

Bu çalışmada, fipronilin farklı dozlarının soğan (Allium cepa) kök ucu hücreleri üzerine sitogenetik etkileri çalışılmıştır. Kökler 5, 10, 15 mg/100 ml fipronil ile 6-12-24 saat muamele edilmiştir ve daha sonra kök ucu hücrelerinin 12, 24, 48 saat iyileştirmeye bırakılarak mitoz bölünmelerindeki değişiklikleri incelenmiştir. Fipronilin doz artışına bağlı olarak kromozom anormalliklerinin arttığı gözlenmiştir.

Anahtar Kelimeler:

böcek öldürücüler, kromozom hataları, mitoz, Allium cepa

The effects of fibronil on the root tıp cells of onion (Allium cepa L.)

In this studies, the cytogenetic effects of fipronil on root tip of Allium cepa, at different concentrations, were studied. The roots were treated with 5, 10, 15 mg/100 ml of the fipronil for 6 and 24 hours and then the root tip cells were examinated in mitosis division variations by recovering for 12, 24, and 48 hours. It was observed that mitotic abnormalites were increased depending on the concentration of fipronil.

Keywords:

insecticides, chromosome aberrations, mitosis, Allium cepa,

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[1] Mohandas, T., Grant, W.F. Cytoıgenetic effects of 2,4 D and amitrol in relation to nuclear volume and DNA cotent in some higher. Can. J. Genet. Cytol., 14:773-778 ,(1972).

[2] Kilhman, B. A. Root tips of Vicia faba for the study of the induction of chromosomal aberrations. Mutation Research, 31:401-412,(1975).

[3] Sinha, R. K., Choudhury, R., Mallick, R. Cytologycal Effects of Phosalone on Root Meristem of Allium cepa L. Cytologia, 54:429-435, (1989).

[4] Butler E.T. and Du Preez I. Laboratory determination of the LD50 and LD90 values for fipronil against fifth instar nymphs of the brown locust Locustana pardalina (Walk.). Agricultural Research Council, Plant Prptection Research Institute, Pretoria, South Africa, (1994).

[5] Kriel C.F., Butler E.T. and Du Preez I. Laboratory determination öf the LD50 and LD90 values for fipronil against fifth instar af rican migratory locust nymphs, Locusta migratoria migratorioides. Agricultural Research Council, Plant Protection Research Institude, Pretoria, South Africa ,(1994).

[6] Megenasa T. and Muinamia C.K. Evaluation of fipronil (EXP. 60145 A) in the control and management of the desert locust {Schistocerca gregaria, Forskal) nymphs and adults. DLCOEA, Nairobi, 30 pp, Kenya ,(1994).

[7] Price R.E., Butler E.T. and Brown H.D. Efficacy of fipronil against nymphal bands of the brown locust in the Karoo, South Africa. Agricultural Research Council, Plant Protection Research Institute, Pretoria, 12 pp. ,(1994).

[8] Rachadi T., Balança G., Duranton J.F. and Foucart A. Les effets du fipronil sur Schistocerca gregaria (Forskal 1775) divers sauteriaux et la faune non-cible. Principaux resultans experimentaux obtenus par le Mauritanie (October a december 1994). D.513, CIRAD-GERDAT-PRIFAS, Montpellier, France, 116 pp. ,(1995).

[9] Balança G. and de Visscher M.N. Impacts on Nontarget Insects of a New Insecticide Compound used Against the Deseryt Locust (Schistocerca gregaria, Forskal 1775). Arch. Environ. Contam. Toxicol. 32, 58-62 France ,(1997-a).

[10] Balança G. and de Visscher M.N. Effects of very low doses of fipronil on grasshoppers and non-target insects following field trials for grasshopper control. Crop Protection, Vol. 16, No. 6pp. 553-564 ,(1997-b).

[11] Kaymak F., Effects Aluminium (Al3+) on the root meristem cells of Allium cepa L. and Allium sativum L. 20: 139-145 (1996)

[12] Khynriam D and Prasad S.B. Effect of cisplatin on Allium cepa root meristem cells. Cytobios, 100,171-180,(1999).

[13] Chauhan L.K.S. and Sundararaman V. Effect of substituted ures on plant cell I. Cytological effects of isoproturon on the root meristem cells of Allium cepa. Cytologia, 55: 91-98, (1990).

[14] Rencüzoğulları E., Kayraldız A., İla H.B., Çakmak T. ve Topaktaş M. The Cytogenetic effects of sodium metabisulfite, a food preservative in root tip cells of Allium cepa. Turk J Biol, 25: 361-370,(2001).

[15] Zakia M. A., Fawzia A. E., Abo-EI-Kheir A. And İman -El- Sheikh A. Alteration in nucleic acids protein content and mitotic division of Vicia faba root tips cells as affected by malathion and tamaron insecticides. Cytologia, 55: 349-355, (1990).

[16] Pedibhotla V.K., Hall F.R., Holmsen J. Deposit characteristics and toxicity of fipronil formulations for tobacco budworm ( Lepidoptera: Noctuidae) control on cotton. Crop Protection, 18: 493-499 ,(1999).

[17] Lecoq M. and Balança G. Field trials of fipronil for control of Rhammatocerus schistocercoides ( Rehn, 1906) hopper bands in Brazil. Crop Protection, Vol. 17, No. 2 pp. 105-110,(1998).

[18] Rachadi T. and Foucart A. L'efficacite du fipronil en barrieres contre les bandes larvaires du criquet pelerin, Schistocerca gregaria (Forskal 1775) en conditions reel les d'operations antiacridiennes. Mauritanie (October november 1995). D.538, CIRAD-GERDAT-PRIFAS, Montpellier, France, 42 pp. ,(1996).

[19] Lockwood J., Schell S., Rockwell R., Isu N. Field test of fipronil for control of rangeland grasshoppers in Wyoming (USA): implications for reduced agentlarea treatments. A Repor to Rhone Pooulenc. University of Wyoming. Laramie WY, USA, 31 pp. ,(1996).