Emine ARSLAN, Leyla AÇIK, Uçkun Sait UÇAN, Ayten ÇELEBİ

Antimicrobial resistance and plasmid typing of coagulase positive Staphylococcus species isolated from bovine mastitis

Bu çalışmada, 50 koagulaz pozitif Staphylococcus izolatı mastitisli ineklerden konventiyonal bakteriyolojik metodlar kullanılarak izole edilmiş ve tanımlanmıştır. Toplam izolattan 40 tanesi Staphylococcus aureus olarak ve 10 tanesi ise Staphylococcus intermedius olarak tanımlanmıştır. Bu çalışma, subklinik mastitisli ineklerin etken ajanlar olarak S. intermedius’un öneminin arttışını göstermiştir. Bütün Staphylococcus suşlarının antibiyotiklere duyarlılıkları ve plazmit profilleri incelenmiştir. Plazmit profili 40 S. aureus izolatının 38’nin ve 10 S. intermedius izolatının 9’nun plazmit içerdiğini göstermiştir. Plazmitlerin moleküler ağırlıkları 1.1 den 19 kb’a kadar değişmektedir. Kullanılan kemoterapotiklere en yüksek dirençlilik penisilinde gözlenmiştir. Bu çalışma, plazmit profili analizinin S. aureus ve S. intermedius’un tiplendirilmesi için uygun bir metod olmadığını göstermektedir.

Anahtar Kelimeler:

Staphylococcus aureus, Staphylococcus intermedius, meme yangısı, metodoloji, inek, plazmitler

Mastitisli ineklerden izole edilen koagulaz pozitif Staphylococcus türlerinin antimikrobiyal dirençliliği ve plazmit tiplendirmesi

In this study, a number of 50 Coagulase positive Staphylococcus isolates has been isolated and identified using conventional bacteriological methods from bovine mastitis. Of the total isolates 40 was identified as Staphylococcus aureus and 10 was identified as Staphylococcus intermedius. This study indicates the increasing significance of S.intermedius as the causative agents of bovine subclinical mastitis. All the Staphylococcus strains were examined for their susceptibilities to antibiotics and plasmid profiles. Plasmid profiling demonstrated that 38 of 40 S.aureus and 9 of 10 S.intermedius isolates contained plasmid. Molecular weight of plasmids varied from 1.1 to 19 kb. The highest resistance to chematerapotics used was observed to penicillin. This study suggests that plasmid profile analysis does not appear to be a suitable method for typing S.aureus and S.intermedius.

Keywords:

Staphylococcus aureus, Staphylococcus intermedius, mastitis, methodology, cows, plasmids,

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