COMPARISON OF LATEX AGGLUTINATION AND ELISA FOR DETECTION OF CLOSTRIDIUM DIFFICILE IN PATIENTS WITH DIARRHEA AND ASYMPTOMATIC GROUPS

Bu çalışmada, diyare ve diğer gastrointestinal yakınmaları olan 184 hasta (82 kadın, 102 erkek) ve diyare mevcut olmayan 88 asemptomatik hastanın (34 kadın, 54 erkek) dışkı örneği Erciyes Üniversitesi Gevher Nesibe Hastanesi Merkez Laboratuvarı'nda C. difficile açısından ELISA, lateks aglütinasyon (LA) ve kültür yöntemleri ile değerlendirilmiştir. LA ile 184 dışkı örneğinin 17'sinde (%9.2) C. difficile varlığı saptanmıştır. Kontrol grubunda yer alan 88 örneğin üçü (%3.4) LA ile pozitif sonuç vermiştir. Örnekler C. difficile toksin A açısından da test edilmiştir. ELISA ile pozitiflik oranı %6.5 (184 örneğin 12'si) olarak bulunurken kontrol grubunda yer alan 88 asemptomatik hastada pozitiflik saptanmamıştır. ELISA toksin A testi standart bir yöntem olarak kabul edildiğinde LA testinin duyarlılığı %100, özgüllüğü %97, pozitif prediktif değeri %60 ve negatif prediktif değeri %100 olarak bulunmuştur.

DİYARELİ HASTALAR VE ASEMPTOMATİK GRUPLARDA CLOSTRIDIUM DIFFICILE'NİN SAPTANMASINDA LATEKS AGLÜTİNASYONU VE ELISA'NIN KARŞILAŞTIRILMASI

In this study, stool specimens from 184 patients with diarrhea (82 females, 102 males), who also complained of other gastrointestinal symptoms and from the control group of 88 asymptomatic nondiarrheal patients (34 females, 54 males) were tested for C. difficile by using enzyme-linked immunosorbent assay (ELISA), latex agglutination (LA), and culture in our central laboratory of Erciyes University Gevher Nesibe Hospital. LA test showed that C. difficile colonization was positive in 17 of 184 stool specimens (9.2%) from patients with diarrhea examined. In the control group, three of 88 stool samples (3.4 %) were positive by LA. The specimens were also tested for presence of toxin A of the bacterium. The positive rate by ELISA was 6.5% (12 of 184) in the patients with diarrhea. But all of the 88 asymptomatic controls were found to be negative by ELISA. When ELISA toxin A assay was accepted as standard test, the sensitivity of LA test was determined to be 100%, its specifity was 97%, the predictive value of a positive test was 60%, and the predictive value of a negative test was 100%.

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