Nilgün ALTIN, Kamer KOLDAŞ, Göknur TOROS YAPAR, Gülkan SOLGUN, İrfan ŞENCAN, Salih CESUR

Çeşitli Klinik Örneklerden İzole Edilen Staphylococcus aureus Suşlarında Metisilin Direncinin Belirlenmesinde Chrom-ID MRSA Besiyerinin Değerlendirilmesi

Amaç: Bu çalışmada, Etlik İhtisas Eğitim ve Araştırma Hastanesinde yatan hastane enfeksiyonu olan hastalardan izo- le edilen metisiline dirençli Staphylococcus aureus (MRSA) olarak tanımlanan 80 suş ile metisiline duyarlı S.aureus(MSSA) olarak tanımlanan 40 suş olmak üzere toplam 120 S.aureus suşunda Chrom ID MRSA agar besiyerininduyarlılık ve özgüllüğünün belirlenmesi amaçlandı.Yöntem ve Gereçler: Staphylococcus aureus suşlarında metisilin direnci sefoksitin disk difüzyon yöntemi veVITEK-2 otomatize sitemi ile belirlendi.Bulgular: Chrom ID MRSA agar besiyerinin 12., 24., 48. ve 72. saattlerdeki sensitivite ve spesifitesi sırasıyla; %70,%60; %85, %75; %92.5, %80 olarak belirlendi. İnkübasyon süresi kırksekiz saate kadar uzatıldığında Chrom ID MRSA agar besiyerinin duyarlılık ve özgüllükdeğerle¬rinin arttığı, kırksekiz saatten fazla inkübasyonun ise duyarlılık ve özgüllükte bir artışa neden olmadığıbelirlendi.Sonuç: Sonuç olarak, kromojen besiyerlerinin maliyetlerinin yüksek olduğu göz önüne alındığında konvansiyonelyöntemlere (sefoksitin ve oksasilin disk difüzyon yöntemi) göre yaklaşık 12 ile 24 saat erken sonuç elde etmenin çokfazla avantajlı olmadığı görüşüne varılmıştır.

Evaluation of Chrom ID MRSA Agar In Determining Methicillin Resistance of Staphylococcus aureus Species Isolated From Various Clinical Specimens

Aim: The aim of this study was to determine the sensitivity and specifity of Chrom ID MRSA agar in 80 methicillinresistance Staphylococcus aureus (MRSA) and 40 methicillin susceptible Staphylococcus aureus isolated from inpa- tients with nosocomial infection.Material and Methods: Methicillin resistance in Staphylococcus aureus strains was determined by cephoxitin discdiffusion method and VITEC 2 automathized system. Results: The sensitivity and specifity of Chrom ID MRSA agar at 12th, 24th, 48 th and 72 th hours were detected as70%, 60%; 85%, 75%; 92.5%, 80%, respectively. When incubation period of Chrom ID MRSA agar was prolonged up to 48 hours, sensitivity abd specifity were in- creased wheras when it was prolonged more than 48 hours sensitivity and specifity were not affected. Conclusion: As a results , chromogenic agar such as Chrom ID MRSA agar were not advantages than conventionalmethods because it is more expencive although it give results earlier up to 12-24 hours.

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