Chlorine and ozone applications used for fruit and vegetable disinfection in tourism accommodation facilities

Samples of vegetable and fruit were taken from the kitchens of tourism accommodation facilities and microbiological analyses were made. The samples were analysed after being disinfected with 100 ppm chlorine for 2-10 minutes and with 2 ppm ozone for 2-10 minutes. In the examined vegetables and fruit samples, Escherichia coli, E. coli O157, Salmonella spp., Listeria monocytogenes were examined in terms of microorganisms. Before disinfection, E. coli was detected in all 30 samples: E. coli O157 in 8 samples, Salmonella spp. in 4 samples, and L. monocytogenes in 3 samples. As a result of disinfection applications with 100 ppm chlorine for 2 minutes, E. coli decreased by more than 45%. Salmonella spp. was detected in 1 sample and E. coli O157 was detected in 2 samples. After disinfection applications with 2 ppm ozone for 2 minutes, E. coli decreased by more than 60%. A decrease of more than 70% was observed in E. coli. after disinfection applications with 100 ppm chlorine for 5 minutes. As a result of disinfection applications with 2 ppm ozone for 5 minutes, E. coli decreased by more than 85%. No bacteria were found in the samples after disinfection with 100 ppm chlorine and 2 ppm ozone for 10 minutes. It was observed that there was a decrease in the microorganism loads as a result of the chlorination and ozonation processes of the vegetable and fruit samples.
Anahtar Kelimeler:

Food, Chlorine, Disinfection, Ozone

Chlorine and ozone applications used for fruit and vegetable disinfection in tourism accommodation facilities

Samples of vegetable and fruit were taken from the kitchens of tourism accommodation facilities and microbiological analyses were made. The samples were analysed after being disinfected with 100 ppm chlorine for 2-10 minutes and with 2 ppm ozone for 2-10 minutes. In the examined vegetables and fruit samples, Escherichia coli, E. coli O157, Salmonella spp., Listeria monocytogenes were examined in terms of microorganisms. Before disinfection, E. coli was detected in all 30 samples: E. coli O157 in 8 samples, Salmonella spp. in 4 samples, and L. monocytogenes in 3 samples. As a result of disinfection applications with 100 ppm chlorine for 2 minutes, E. coli decreased by more than 45%. Salmonella spp. was detected in 1 sample and E. coli O157 was detected in 2 samples. After disinfection applications with 2 ppm ozone for 2 minutes, E. coli decreased by more than 60%. A decrease of more than 70% was observed in E. coli. after disinfection applications with 100 ppm chlorine for 5 minutes. As a result of disinfection applications with 2 ppm ozone for 5 minutes, E. coli decreased by more than 85%. No bacteria were found in the samples after disinfection with 100 ppm chlorine and 2 ppm ozone for 10 minutes. It was observed that there was a decrease in the microorganism loads as a result of the chlorination and ozonation processes of the vegetable and fruit samples.

___

  • Aruscavage D, Lee K, Miller S and LeJeune JT (2006) Interactions affecting the proliferation and control of human pathogens on edible plants. Journal of Food Science 71: 89-99.
  • Aytemiş Z (2021) Turp mikroyeşilliklerinde Salmonella enterica typhimurium ve Escherichia coli O157:H7’nin klorlu su ile sprey sulama sırasında dezenfekte edilmesi. Yüksek Lisans Tezi, Alparslan Üniversitesi Fen Bilimleri Enstitüsü, Muş.
  • Behrsing J, Winkler S, Franz P, Premier R (2000) Efficacy of chlorine for inactivation of Escherichia coli on vegetables. Postharvest Biology and Technology 19: 187-92.
  • Delaquis PJ, Stewart S, Toivonen PMA and Molys AL (1999) Effect of warm chlorinated water on the microbial flora of shredded iceberg lettuce. Food Research International 32: 553-563.
  • Erkoç Ö (2019) Çiğ olarak tüketilen bazı salata malzemelerinin mikrobiyolojik yönden incelenmesi. Yüksek Lisans Tezi, Hacettepe Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara.
  • Gökçe R (2011) Antimikrobiyal Maddelerle Gıdaların Korunması. (Ed: Osman Erkmen), Gıda Mikrobiyolojisi, Elif Yayınevi, Ankara, s. 234-260.
  • Gölgeçen İ (2014) Kirazın raf ömrünün arttırılmasında klor dioksit uygulamasının etkinliği. Yüksek Lisans Tezi, Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü, Çanakkale.
  • Gücükoğlu A, Küplülü Ö (2005) Su ve gıda güvenliğinin sağlanmasında ozon kullanımı. Akademik Gıda Dergisi 3: 5-9.
  • Karaca H (2010) Ozonlamanın marul, ıspanak ve maydanozlarda mikrobiyel inaktivasyon ve raf ömrü üzerine etkileri. Doktora Tezi, Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Kemiteks Kimya (2021a) Microgen GN-ID A. www.kemitekskimya.com.tr /files/10122a84a3mid67.pdf. Accessed 18 October, 2021.
  • Kemiteks Kimya (2021b) Microgen GN-ID A. https://www. kemitekskimya.com.tr /files/10120459a2mid64.pdf. Accessed 18 October, 2021.
  • Nou X and Luo Y (2010) Whole-leaf wash improves chlorine efficacy for microbial reduction and prevents pathogen cross-contamination during fresh-cut lettuce processing. Journal of Food Science 75: 283-90.
  • Savaş E, Tavşanlı H, Gökgözoğlu İ (2014) Gıda endüstrisinde ozon uygulamaları. Türk Tarım–Gıda Bilim ve Teknoloji Dergisi 2: 122-127.
  • Sevilgen Ö (2009) Ozon, klor ve hidrojen peroksit uygulamalarının pazıda klorofil miktarı üzerine etkileri. Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Tetik N, Topuz A, Turhan İ, Tarhan M (2006) Meyve ve sebzelerin işlenmesi ve muhafazasında ozon uygulamaları. Türkiye 9. Gıda Kongresi, Bolu, Türkiye, pp. 281-284.
  • TSE (2003) TS EN ISO 16654, Gıda ve Hayvan Yemlerinin Mikrobiyolojisi-Eschericha coli O157'nin Tespiti için Yatay Yöntem.
  • TSE (2012) TS ISO 16649-2, Gıda ve Hayvan Yemleri Mikrobiyolojisi-Beta-Glucuronidase-Positive Escherichia coli'nın Sayımı için Yatay Yöntem-Bölüm 2: 5-Bromo-4-Chloro-3-İndolyl Beta-D-Glucuronide Kullanılarak 44°C'da Koloni Sayım Yöntemi.
  • TSE (2017a) TS EN ISO 11290-1, Gıda Zinciri Mikrobiyolojisi – Listeria monocytogenes ve Listeria spp.’nin Aranması ve Sayımı için Yatay Metod Bölüm 1: Arama Metodu.
  • TSE (2017b) TS EN ISO 6579-1, Besin Zincirinin Mikrobiyolojisi – Salmonella’nın Tespiti, Sayımı ve Serotiplendirilmesi için Yatay Yöntem – Bölüm: 1: Salmonella spp.
  • Tümay M (2019) Ozonun, bazı yeşil yapraklı sebzelerde antibakteriyel etkisinin belirlenmesi. Yüksek Lisans Tezi, Hacı Bektaş Veli Üniversitesi, Nevşehir.
  • Türk Gıda Kodeksi Mikrobiyolojik Kriterler Yönetmeliği (2011) Resmi Gazete, 28157.
  • Xu L (1999) Use of ozone to improve the safety of fresh fruits and vegetables. Food Technology 53: 58-63.
Mediterranean Agricultural Sciences-Cover
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1988
  • Yayıncı: Akdeniz Üniversitesi