Süleyman KÖK, Sertaç ATALAY, Güldan VAPUR, M. İhsan SOYSAL

Sığırlarda Kalpain ve Kalpastatin Gen Polimorfizmlerinin Et Tekstürünün İyileştirilmesi Çalışmalarında Kullanımı

Son yıllarda, tüketici tercihleri doğrultusunda et tekstürünün iyileştirilmesi konusuna ilgi artmış ve özellikte etçi sığır ırklarında bu özelliğini iyileştirilmesine yönelik çalışmalar yapılmıştır. Etin gevrekliğinden üç enzim; μ- kalpain (CAPN1) ve m-kalpain (CAPN2) ile bunların inhibitörü olan kalpastatin (CAST) sorumludur. Yapılan çok sayıda çalışmada, kalpain-kalpastatin sisteminin normal iskelet kası gelişimi için önemli olduğu ve CAST ile CAPN1 lokuslarındaki bazı SNP’lerin et tekstürü ile ilişkili olduğu bildirilmiştir. Çalışmaların çoğunda, CAST ve CAPN1 gen polimorfizmleri ile musculus longissimus dorsi (MLD) kasının Warner Bratzler Shear Force (WBSF) tekstür bıçağının kesme direnci arasında bir ilişkinin olduğu belirlenmiştir. Et sığırcılığında, kalpain geni (CAPN1 316, CAPN1 530 ile CAPN1 4751) ve kalpastatin geni (UoG-CAST, CAST-T1) ile bu genlerdeki varyantlar et tekstürü için aday gen olarak kabul edilmektedir. Sığır eti kalite özelliklerinin erken yaşta belirlenmesi için bu SNP’ler önemli bir fırsat sağlamaktadır. Genetik markır teknolojisinin, çiftlik hayvanlarının genetik gelişimi için gelecek vaad eden umut verici bir araç olduğu düşünülmektedir.

Anahtar Kelimeler:

CAPN1, CAST, et tekstürü

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1. Alireza M, Jothi MP, Awis QS, Siti S (2009): Characterization of bovine calpastatin gene in Nelore cattle using polymerase chain reaction-restricted fragment length polymorphisms. American Journal of Animal and Veterinary Sciences. 4(4): 92-94.
2. Allais S, Journaux L, Levéziel H, Payet-Duprat N, Raynaud P, Hocquette JF, Lepetit J, Rousset S, DenoyelleC, Bernard-Capel C (2011): Effects of polymorphisms in the calpastatin and μ-calpain genes on meat tenderness in 3 French beef breeds. Journal of animal science. 89(1): 1-11.
3. Anonim, (2019) Development of genetic and physical marker maps of the bovinegenome (Bovine Gene Mapping project). https://cordis. europa.eu/project/rcn/5322/factsheet/en. Erşim 13.07.2019
4. Barendse W (2002): DNA markers for meat tenderness. International patent application No. 2002, PCT/AU02/00122. World Intellectual Property Org. Int. Publication No. WO 02/064820.
5. Barendse WG (2009): DNA markers for meat tenderness. United States Patent 625698.
6. Barendse WG, Harrison BE, Hawken RJ, Ferguson DM, Thompson JM, Thomas MB, Bunch RJ (2007): Epistasis between calpain 1 and its inhibitor calpastatin within breeds of cattle. Genetics. 176(4): 2601-2610.
7. Bishop M, Koohmaraie M, Killefer J, Kappes S (1993): Rapid communication: restriction fragment length polymorphisms in the bovine calpastatin gene. Journal of animal science. 71(8): 2277.
8. Casas E, White S, Riley D, Smith T, Brenneman R, Olson T, Johnson D, Coleman S, Bennett G, Chase Jr C, (2005): Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. Journal of animal science. 83(1):13-19.
9. Casas E, White S, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase Jr C, Johnson D, Smith T (2006): Effects of calpastatin and μ-calpain markers in beef cattle on tenderness traits. Journal of Animal Science. 84(3): 520-525.
10. Cockett NE, Shay TL, Green RD, Hancock DL (1995): Rapid communication: a TaqI restriction fragment length polymorphism in the bovine calpastatin gene. J. Anim. Sci. 73: 3790.
11. Corva PM, Soria L, Schor A, Villarreal EL, Cenci MP, Motter M, Mezzadra C, Melucci LM, Miquel C, Paván E (2007): Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina. Genetics and Molecular Biology. 30(4): 1064-1069.
12. Costello S, O’Doherty E, Troy D, Ernst C, Kim KS, Stapleton P, Sweeney T, Mullen A (2007): Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus dorsi. Meat Science. 75(4): 551-557.
13. Curi RA, Chardulo LAL, Giusti J, Silveira AC, Martins CL, de Oliveira HN (2010): Assessment of GH1, CAPN1 and CAST polymorphisms as markers of carcass and meat traits in Bos indicus and Bos taurus–Bos indicus cross beef cattle. Meat Science. 86(4): 915-920.
14. Curi RA, CharduloLAL, MasonM, ArrigoniM, SilveiraAC, de Oliveira HN (2009): Effect of single nucleotide polymorphisms of CAPN1 and CAST genes on meat traits in Nellore beef cattle (Bos indicus) and in their crosses with Bos taurus. Animal genetics. 40(4): 456-462.
15. Djadid ND, Nikmard M, Zakeri S, Gholizadeh S (2011): Characterization of calpastatin gene in Iranian Afshari sheep. Iran J Biotechnol. 9(2): 145-149.
16. Eenennaam AV (2006): Marker assisted selection in beef cattle. Department of Animal Science. University of California. Davis, CA USA.
17. Elmacı C, Öner Y (2007): Et sığırcılığında moleküler genetik yaklaşımlar. Hayvansal Üretim. 48(2).
18. Frylinck L, van Wyk G, Smith TP, Strydom PE, van Marle-Köster E, Webb EC, Koohmaraie M, Smith MF (2009): Evaluation of biochemical parameters and genetic markers for association with meat tenderness in South African feedlot cattle. Meat science. 83(4): 657-665.
19. Gigli S. Failla S, Iacurto M, Contò M, Sañudo C, Olleta JL, Albertí P, Panea B, Hocquette JF, Richardson I, Ertbjerg P, Christensen M, Nute GR, Williams JL (2006): Performance at slaughter and beef quality characteristics of some Mediterranean beef breeds compared to Central and North European breeds (GemQual EU Project). Mediterranean livestock production: Uncertainties and opportunies: CIHEAM / CITA. A series, No:78, Pp 173-182, May 18-20, Zaragoza, Spain
20. Gill JL, Bishop SC, Mc Corquodale C, Williams JL, Wiener P (2009): Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired beef cattle. Genetics Selection Evolution. 41(1): 36.
21. Gollapudi G (1999): Evaluation of growth hormone as acandidate gene in dairy cattle breeding. Mc Gill University, Canada.
22. Gökalp HY, Kaya M, Tülek Y, Zorba Ö (1995): Et ve Ürünlerinde Kalite Kontrolü ve Laboratuvar Uygulama Klavuzu. Atatürk Üniversitesi Yayınları No:751. Erzurum
23. Gupta M, Chyı YS., Romero-Severson J, Owen JL (1994): Amplification of DNA markers from evalutionarily diverse genomes using single primers of simplesequence repeats. Theoretical and Applied Genetics. 89 (7-8): 998-1006
24. Hocquette JF, Renand G, Levéziel H, Picard B, Cassar-Malek I (2005): Genetic effects on beef meat quality. The Science of Beef Quality, 8th Annual Langford Food Industry Conference, Proceedings of the British Society of Animal Science. Pp 13-19, May 18 – 19, Langford, Kanada.
25. Iso-Touru T, Pesonen M, Fischer D, Huuskonen A, Sironen A (2018): The effect of CAPN1 and CAST gene variations on meat quality traits in Finnish Aberdeen Angus and Nordic Red Cattle populations. Agricultural and Food Science, 27 (4): 227–231. https://doi.org/10.23986/afsci.75125
26. Juszczuk-Kubiak E, Flisikowski K, Wicińska K, Połoszynowicz J, Rosochacki S (2009): Identification of the new polymorphisms in the promoter region of the CAST gene in cattle. Meat science. 82(2): 278-283.
27. Juszczuk-Kubiak E, Słoniewski K, Oprządek J,Wicińska K, Połoszynowicz J, Rosochacki S (2009): The effect of polymorphisms in the intron 12 of CAST gene on meat quality of young bulls. Animal Science Papers and Reports. 27(4): 281-292.
28. Juszczuk-Kubiak E, Wyszyńska-Koko J, Wicińska K, Rosochacki S (2008): A novel polymorphisms in intron 12 of the bovine calpastatin gene. Molecular biology reports. 35(1): 29-35.
29. Koohmaraie M (1994): Muscle proteinases and meat aging. Meat science. 36(1-2): 93-104.
30. Koohmaraie M (1996): Biochemical factors regulating the toughening and tenderization processes of meat. Meat science. 43: 193- 201.
31. Koohmaraie M, Whipple G, Kretchmar D, CrouseJ, MersmannH (1991): Postmortem proteolysis in longissimus muscle from beef, lamb and pork carcasses. Journal of Animal Science. 69(2): 617- 624.
32. Kök S, Atalay S (2018): The Use of Various SNPs in CAST and CAPN1 Genes to Determine the Meat Tenderness in Turkish Grey Cattle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 24(1): 1-8.
33. Kök S, Atalay S, Eken HS, Savasçı M (2017): The genetic characterization of Turkish grey cattle with regard to UoG Cast, CAPN1 316 and CAPN1 4751 markers. Pakistan Journal of Zoology. 49(1).
34. Kök S, Atalay S, Savaşçı M, Eken HS (2013): Characterization of calpastatin gene in purebred and crossbred Turkish Grey Steppe cattle. Kafkas Univ Vet Fak Derg. 19(2): 203-206.
35. Lonergan SM, Ernst C, Bishop M, Calkins CR, Koohmaraie M (1995): Relationship of restriction fragment length polymorphisms (RFLP) at the bovine calpastatin locus to calpastatin activity and meat tenderness. Journal of animal science. 73(12): 3608-3612.
36. Miller M, Huffman K, Gilbert S, Hamman L, Ramsey C (1995): Retail consumer acceptance of beef tenderized with calcium chloride. Journal of animal science. 73(8): 2308-2314.
37. Morris C, Cullen N, Hickey S, Dobbie P, Veenvliet B, Manley T, Pitchford W, Kruk Z, Bottema C, Wilson T (2006): Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey× Limousin, Angus and Hereford-cross cattle. Animal genetics. 37(4): 411-414.
38. Negrini R, Nicoloso L, Crepaldi P, Milanesi E, Colli L, Chegdani F, Pariset L, Dunner S, Leveziel H, Williams JL (2008): Assessing SNP markers for assigning individuals to cattle populations. Animal Genetics. 40: 18–26. https://doi.org/10.1111/j.1365- 2052.2008.01800.x
39. Özbeyaz C, Bayraktar M, Alpan O, Akcan A (1991): Jerseylerde süt protein polimorfizmi ve ilk laktasyon süt verimiyle İlişkisi. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi. 31(3-4): 27-33.
40. Page B, Casas E, Quaas R, Thallman R, Wheeler T, Shackelford S, Koohmaraie M, White S, Bennett G, Keele JW (2004): Association of markers in the bovine CAPN1 gene with meat tenderness in large crossbred populations that sample influential industry sires. Journal of animal science. 82(12): 3474-3481.
41. Page B, Casas E, Heaton M, Cullen N, Hyndman D, Morris C, Crawford A, Wheeler T, Koohmaraie M, Keele JW (2002): Evaluation of single-nucleotide polymorphisms in CAPN1 for association with meat tenderness in cattle. Journal of animal science. 80(12): 3077-3085.
42. Quaas R, Li J, Thallman R, Van Eenennaam A, Fernando R, Gill C (2006) Validation of commercial DNA tests for quantitative beef traits. in 8th World Congress on Genetics Applied to Livestock Production.13–18 August, Belo Horizonte, MG, Brasil.
43. Reardon W, Mullen A, Sweeney T, Hamill R (2010): Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus. Meat Science. 86(2): 270-275.
44. Rincon G, Medrano JF (2006): Assays for genotyping single nucleotide polymorphisms in the bovine CAPN1 gene. Animal genetics. 37(3).
45. Schenkel FS, Miller SP, Jiang Z, Mandell IB, Ye X, Li H, Wilton JW (2006): Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. Journal of animal science. 84(2): 291-299.
46. Smith TPL, Casas CE, Rexroad C, Kappes CM, Keele JW (2000): Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness. Journal of animal science. 78(10): 2589-2594.
47. Smith TPL, Thomas M, Bidner T, Paschal J, Franke D (2009): Single nucleotide polymorphisms in Brahman steers and their association with carcass and tenderness traits. Genetics and Molecular Research. 8(1): 39-46.
48. Van Eenennaam AL, Li J, Thallman RM, Quaas RL, Dikeman ME, Gill CA, Franke DE, Thomas MG (2007): Validation of commercial DNA tests for quantitative beef quality traits. Journal of animal science. 85(4): 891-900.
49. Weaber RL, Lusk JL (2010): The economic value of improvements in beef tenderness by genetic marker selection. American Journal of Agricultural Economics. 92(5): 1456-1471.
50. Wheeler TL, Shackelford SD, Koohmaraie M (1997): Standardizing collection and interpretation of Warner-Bratzler shear force and sensory tenderness data. In Proceedings 50th annual reciprocal meat conference, Pp 68-77, Ames, IA.
51. White S, Casas C E, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase Jr C, Johnson D, Keele JW, Smith T (2005): A new single nucleotide polymorphism in CAPN1 extends the current tenderness marker test to include cattle of Bos indicus, Bos taurus, and crossbred descent. Journal of animal science. 83(9): 2001- 2008.
52. Xiaomei S, Xiuxiang W, Yongliang F, Yongjiang M, Dejun J, Bizhi H, Zhangping Y (2018): Effects of polymorphisms in CAPN1 and CAST genes on meat tenderness of Chinese Simmental cattle. Archives Animal Breeding, 61: 433-439.
53. Yousefi S, Azari MA (2012): Study of Calpastatin Gene Polymorphism in Holstein Cattle and Buffalo. Anim. Sci. Biotech. 45: 285-288.