Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey
The objective of the present study was to determine genotypic/allelic frequencies and population genetic indices of CAPN1 g.5709 C>G and MYF5 g.1911 A>G polymorphisms in beef cattle imported from Brazil to Turkey. Single nucleotide polymorphisms were carried out using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. A total of 108 bulls, including Angus, Angus×Hereford×Nellore, Brahman, Hereford, Limousine, and Charolais breeds, were genotyped. Concerning the CAPN1, CC genotype was not found in this study. Besides, the G allele frequency was quite high (0.75). Regarding the MYF5, frequency of AA genotype was rather low (4.63%) compared to the other two genotypes, AG and GG. Therefore, the frequency of A allele was quite low (0.21). On the basis of breed-specific evaluation, the highest frequency of GG genotype of the CAPN1 was found in Brahman breed (88.24%) whereas the highest frequency of heterozygous genotype was determined in Charolais breed (85.71%). The frequency of MYF5 GG genotype was found to be very high in Limousine breed (75.00%). Moreover, MYF5 AA genotype was absent in Angus, Brahman, Limousine, and Charolais breeds. The present study may be useful for further genetic analyses conducted on beef cattle imported into Turkey.
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- 1. Allais S, Journaux L, Leveziel H, Payet-Duprat N, Raynaud P,
Hocquette JF, Lepetit J, Rousset S, Denoyelle C, Bernard-Capel
C, Renand G (2011): Effects of polymorphisms in the calpastatin
and mu-calpain genes on meat tenderness in 3 French beef breeds.
Journal of Animal Science, 89: 1-11.
- 2. Ardicli S, Samli H, Dincel D, Soyudal B, Balci F (2017): Individual
and combined effects of CAPN1, CAST, LEP and GHR gene polymorphisms
on carcass characteristics and meat quality in Holstein
bulls. Archives Animal Breeding, 60: 303-313.
- 3. Ardicli S, Samli H, Vatansever B, Soyudal B, Dincel D, Balci F
(2019): Comprehensive assessment of candidate genes associated
with fattening performance in Holstein–Friesian bulls. Archives
Animal Breeding, 62: 9-32.
- 4. Bhuiyan MSA, Kim N, Cho Y, Yoon D, Kim KS, Jeon JT, Lee JH
(2009): Identification of SNPs in MYOD gene family and their
associations with carcass traits in cattle. Livestock Science, 126:
292-297.
- 5. Botstein D, White RL, Skolnick M, Davis RW (1980): Construction
of a genetic linkage map in man using restriction fragment length
polymorphisms. American Journal of Human Genetics, 32: 314-
331.
- 6. Casas E, Shackelford SD, Keele JW, Koohmaraie M, Smith TP,
Stone RT (2003): Detection of quantitative trait loci for growth
and carcass composition in cattle. Journal of Animal Science, 81:
2976-2983.
- 7. Casas E, White SN, Riley DG, Smith TP, Brenneman RA, Olson
TA, Johnson DD, Coleman SW, Bennett GL, Chase CC (2005):
Assessment of single nucleotide polymorphisms in genes residing
on chromosomes 14 and 29 for association with carcass composition
traits in Bos indicus cattle. Journal of Animal Science, 83:
13-19.
- 8. Curi RA, Chardulo LAL, Giusti J, Silveira AC, Martins CL, de
Oliveira HN (2010): Assessment of GH1, CAPN1 and CAST polymorphisms
as markers of carcass and meat traits in Bos indicus
and Bos taurus–Bos indicus cross beef cattle. Meat Science, 86:
915-920.
- 9. Curi RA, Krauskopf MM, Hadlich JC, Fortes MRS, Vankan DM,
Augusto J, Silva V, de Oliveira HN, da Mota MDS (2012):
Candidate SNPs for carcass and meat traits in Nelore animals and
in their crosses with Bos taurus. Pesquisa Agropecuária Brasileira,
47: 294-301.
- 10. Falconer DS (1960): Introduction to quantitative genetics. Oliver
and Boyd Ltd, Edinburgh, Great Britain, p: 58.
- 11. Gao Y, Zhang R, Hu X, Li N (2007): Application of genomic technologies
to the improvement of meat quality of farm animals. Meat
Science, 77: 36-45.
- 12. Gill JL, Bishop SC, McCorquodale C, Williams JL, Wiener P (2009):
Association of selected SNP with carcass and taste panel assessed
meat quality traits in a commercial population of Aberdeen Angussired
beef cattle. Genetics, Selection, Evolution, 41: 36-47.
- 13. Kisacova J, Kubek A, Melus V, Canakyova Z, Rehout V (2009):
Genetic polymorphism of Myf-5 and myostatin in Charolais breed.
Journal of Agrobiology, 26: 7-11.
- 14. Lacorte G, Machado M, Martinez M, Campos A, Maciel R,
Verneque RS, Teodoro RL, Peixoto MGCD, Carvalho MRS,
Fonseca CG (2006): DGAT1 K232A polymorphism in Brazilian
cattle breeds. Genetics and Molecular Research, 5: 475-482.
- 15. Le Hir H, Nott A, Moore MJ (2003): How introns influence and
enhance eukaryotic gene expression. Trends in Biochemical
Sciences, 28: 215-220.
- 16. Li C, Basarab J, Snelling W, Benkel B, Murdoch B, Hansen C,
Moore SS (2004): Assessment of positional candidate genes myf 5
and igf 1 for growth on bovine chromosome 5 in commercial lines
of Bos taurus. Journal of Animal Science, 82: 1-7.
- 17. Li X, Ekerljung M, Lundstrom K, Lunden A (2013): Association of
polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes
with color, marbling and water holding capacity in meat from beef
cattle populations in Sweden. Meat Science, 94: 153-158.
- 18. Lisa C, Di Stasio L (2009): Variability of μ-calpain and calpastatin
genes in cattle. Italian Journal of Animal Science, 8: 99-101.
19. Miquel MC, Villarreal E, Mezzadra C, Melucci L, Soria L, Corva P,
Schor A (2009): The association of CAPN1 316 marker genotypes
with growth and meat quality traits of steers finished on pasture.
Genetics and Molecular Biology, 32: 491-496.
- 20. Nei M, Roychoudhury A (1974): Sampling variances of heterozygosity
and genetic distance. Genetics, 76: 379-390.
- 21. Page BT, Casas E, Heaton MP, Cullen NG, Hyndman DL, Morris
CA, Crawford AM, Wheeler TL, Koohmaraie M, Keele JW, Smith
TPL (2002): Evaluation of single-nucleotide polymorphisms in
CAPN1 for association with meat tenderness in cattle. Journal of
Animal Science, 80: 3077-3085.
- 22. Pintos D, Corva PM (2011): Association between molecular markers
for beef tenderness and growth traits in Argentinian angus cattle.
Animal Genetics, 42: 329-332.
- 23. Sambrook J, Russell DW (2006): Isolation of high-molecularweight
DNA from mammalian cells using formamide, chapter
6.13. In Cold Spring Harbor protocols, 3rd ed. Cold Spring Harbor
Laboratory Press, Cold Spring Harbor, NY. doi:10.1101/pdb.
prot3225.
- 24. Seong J, Oh JD, Cheong IC, Lee KW, Lee HK, Suh DS, Jeon GJ,
Park KD, Kong HS (2011): Association between polymorphisms
of Myf5 and POU1F1 genes with growth and carcass traits in
Hanwoo (Korean cattle). Genes & Genomics, 33: 425-430.
- 25. Smith T, Thomas M, Bidner T, Paschal J, Franke D (2009): Single
nucleotide polymorphisms in Brahman steers and their association
with carcass and tenderness traits. Genetics and Molecular
Research, 8: 39-46.
- 26. Trakovicka A, Moravcikova N, Kasarda R (2013): Genetic polymorphisms
of leptin and leptin receptor genes in relation with production
and reproduction traits in cattle. Acta Biochimica Polonica,
60: 783–787.
- 27. Yeh FC, Yang RC, Boyle TB, Ye Z, Mao JX (2000): POPGENE, the
user-friendly shareware for population genetic analysis. Molecular
biology and biotechnology centre, University of Alberta, Canada.
- 28. Zhang RF, Chen H, Lei CZ, Zhang CL, Lan XY, Zhang YD, Zhang
HJ, Bao B, Niu H, Wang XZ (2007): Association between polymorphisms
of MSTN and MYF5 genes and growth traits in three
Chinese cattle breeds. Asian-Australasian Journal of Animal
Sciences, 20: 1798-1804.