Merve BÜYÜKAKKAŞLAR, İlker YÜCE, Tuğba BAŞKONUŞ, Tevrican DOKUYUCU, AYDIN AKKAYA, ZİYA DUMLUPINAR

Makarnalık Buğday (Triticum durum Desf.) B27 × Ege 88 Resiprokal Melez Popülasyonunda F4 Kuşağının Allele Özgü Markörlerle Değerlendirilmesi

Makarnalık buğday ıslah çalışmalarında kalite verim ve hastalıklaradayanıklılık önemli ıslah hedefleridir. Bu çalışmada, Türkiye’de bellibir ekim alanına sahip Ege 88 çeşidi ile bazı hastalık ve kaliteözellikleri bakımından iyi olduğu bilinen B27 yerel makarnalıkbuğday çeşidinin resiproklu olarak melezlenmesiyle elde edilen ve F4döl kuşağına getirilen, ebeveynlerle beraber toplam 39 genotip,mumsuluk (Wx-A1), kara pas (Sr49), gluten mukavemeti (Glu-B1),yüksek protein oranı (Gpc-B1), çavdar translokasyonu (1AL.1RS),vernelizasyon (Vrn-A1) ve bin dane ağırlığı özelliklerine ait allelspesifik markörler ile karakterize edilmiştir. Araştırmada kullanılan39 makarnalık buğday genotipi sekiz allel spesifik DNA primeri iletaranmış ve 56 adet polimorfik bant elde edilmiş ve bu datakullanılarak genotiplerin genetik mesafeleri belirlenmiştir. Ortalamabant sayısı 7 olarak bulunurken, en çok bant üreten markör 15 bantile Sun 479, en az bant üreten markör bir bant ile Bx7OE markörüolmuştur. Ortalama polimorfizm bilgi içeriği (PIC) değeri 0.875 olarakhesaplanmıştır. En yüksek PIC değeri 0.99 ile SUN1, SUN479, Bx7OE,RIS ve VRN1 markörlerinden, en düşük PIC değeri ise 0.39 ileUHW89 marköründen elde edilmiştir. Elde edilen markör verileri ileoluşturulan dendrograma göre, Ege 88 × B27_3 genotipinindiğerlerinden genetik olarak uzak olduğu ve Ege 88 × B27_7- Ege 88× B27_8 ve B27 × Ege 88_5 - B27 × Ege 88_6 genotipleri ise % 100benzer bulunmuştur. Makarnalık buğday genotiplerinde mumsuluk(Wx-A1), kara pas (Sr49), yüksek protein oranı (Gpc-B1), glutenmukavemeti (Glu-B1) ve çavdar translokasyonları (1AL.1RS) ile ilgilialleller tespit edilirken, vernelizasyon (Vrn-A1) ve bin tane ağırlığı ileilgili beklenen uzunluklarda bant elde edilememiştir.

Evaluation of F4 Individuals Obtained from B27 × Ege 88 Durum Wheat (Triticum durum Desf.) Reciprocal Cross Population by Allele Specific Markers

Yield, quality and disease resistance are important variables in durum wheat breeding studies. In this study, durum wheat cultivar Ege 88 which have been grown in a certain area in our country and durum wheat landrace B27 which is known as diseases tolerant with high quality properties were crossed as reciprocal and 39 F4 individuals with their parents were screened with allele specific markers for waxiness (Wx-A1), stem rust (Sr49), gluten strength (GluB1), high protein ratio (Gpc-B1), rye translocation (1AL.1RS), vernalisation (Vrn-A1) and thousand kernel weight. In the research, 39 genotypes were screened with eight allele specific markers and produced 56 polymorphic bands and genetic distance of the genotypes were determined. The average allele number was determined as 7, while the most allelic marker was Sun479 with 15 bands and the lowest allele number was obtained from Bx7OE with only one allele. The average polymorphism information content (PIC) was calculated as 0.875. The highest PIC value was obtained from SUN1, SUN479, Bx7OE, RIS and VRN1 markers as 0.99, while the lowest value was calculated as 0.39 from the UHW89 marker. According to the dendrogram created by the marker data Ege 88 × B27_3 genotype was the most diverse one and Ege 88 × B27_7- Ege 88 × B27_8 and B27 × Ege 88_5- B27 × Ege 88_6 genotypes were found 100% identical. The alleles for waxiness (Wx-A1), stem rust (Sr49), high protein ratio (GpcB1), gluten strength (Glu-B1) and rye translocation (1AL.1RS) were found on durum wheat genotypes, while the alleles for vernalisation (Vrn-A1) and thousand kernel weight were not identified.

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