Esra Uçar SÖZMEN, SERAP ŞAHİN BÖLÜKBAŞI, Mustafa ULU, HÜSEYİN AŞKIN AKPULAT

Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ’ndan Elde Edilen Reçinenin In vitro Antioksidan ve Sitotoksik Aktivitelerinin Belirlenmesi

Bu çalışmada, Toros Göknarı bitkisinden elde edilen reçineninantioksidan ve sitotoksik aktivite değerleri değerlendirilmiştir. Bureçine, 2,2′-Azino-bis- (3-etilbenzotiazolin-6-sülfonik asit) (ABTS)radikal süpürme testine göre antioksidan aktiviteye sahiptir.Reçinenin in vitro sitotoksik aktivitesi, bir dizi insan kanser hücresine(MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) karşı MTT (3-(4,5-dimetiltiyazol-2-il) -2,5-difeniltetrazolium bromid) analizi ile 48saat boyunca araştırıldı. Sağlıklı hücreler olarak normal insanakciğer fibroblast hücreleri (WI-38) kullanıldı. Sonuçlar, reçinenin invitro sitotoksik aktivitesinin hücre hattı tipine ve numuneninkonsantrasyonuna bağlı olduğunu göstermiştir. IC50 değerlerine göre,reçine diğer kanser hücrelerine kıyasla endometriyal adenokarsinomkanser hücreleri üzerinde en fazla sitotoksik aktiviteye sahiptir (IC50= 8.94 ± 0.03 µg mL-1). Sonuçlar ayrıca seçicilik indeks (SI) değeri> 2olan Ishikawa endometriyal adenokarsinom hücrelerinin reçineyekarşı en yüksek hassasiyete sahip olduğunu göstermiştir. Bu çalışma,reçinenin farklı kanser hücrelerinin büyümesini engellediğine dair ilkkanıtları sunmaktadır.

The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière)

In this study, the antioxidant and cytotoxic activities of resin obtained from the Cilician Fir plant were evaluated. This resin has antioxidant activity according to 2,2′-Azino-bis-(3-ethylbenzothiazoline-6- sulphonic acid) (ABTS) radical scavenging assay. The in vitro cytotoxic activity of the resin was investigated against a panel of human cancer cells (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay for 48 h. Normal human lung fibroblast cells (WI-38) were used as healthy cells. The results indicated that the in vitro cytotoxic activity of the resin depends on the cell line type and concentration of the resin. According to the IC50 values, the resin has the most cytotoxic activity on endometrial adenocarcinoma cancer cells (IC50=8.94 ± 0.03 µg mL-1) compared to other cancer cells. The results also indicated that Ishikawa endometrial adenocarcinoma cells, which have Selectivity Index (SI) value >2, have the most sensitivity against the resin. This study provides the first evidence that the resin inhibits the different cancer cells' growth.

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