E. Mine SOYLU, Soner SOYLU, Merve KARA, İ. Adem BOZKURT

Tıbbi Bitkilerden Elde Edilen Uçucu Yağların Kimyasal Bileşimi ve Gal Oluşturan Bitki Patojeni Bakteriyel Hastalık Etmenlerine Karşı Antibakteriyel Etkinlikleri

Bu çalışmanın amacı, farklı bitki türlerinden elde edilen uçucu yağların kimyasal bileşimini ve gal (ur) oluşturan bitki patojenik bakteriyel hastalık etmeni Rhizobium radiobacter, Pseudomonas savastanoi pv. savastanoi ve P.savastanoi pv. nerii’ye karşı antibakteriyel etkinlerini belirlemektir. Thymbra spicata var. spicata, Thymus serpyllum, Thymus sipyleus, Origanum syriacum, Origanum majorana, Ocimum basilicum, Mentha spicata, Melissa officinalis, Lavandula stoechas var. stoechas, Rosmarinus officinalis, Salvia officinalis, Laurus nobilis ve Foeniculum vulgare uçucu yağlarının kimyasal bileşenleri GC/MS ile tanımlanmıştır. Carvacrol Thymbra spicata var. spicata (% 66.88) ve Origanum syriacum (% 79.8), thymol Thymus serpyllum (% 41.03), geranial Thymus sipyleus (% 13.72) ve Melissa officinalis (30.4), 4-terpineol Origanum majorana (31.67 %), linalool Ocimum basilicum (% 30.23), carvone Mentha spicata (% 55.58), 1,8 cineole Lavandula stoechas var. stoechas (% 35.5), Laurus nobilis (% 35.5) ve Rosmarinus officinalis (% 18.47), camphor Salvia officinalis (% 24.59) ve trans-anethole ise Foeniculum vulgare (% 82.8) uçucu yağlarının ana bileşenleri olarak belirlenmiştir. Engelleme zon çapı değerlerine dayanarak, uçucu yağlarıP.savastanoi pv. savastanoi (7.0-44.67 mm), ardından R.radiobacter (9.6-37.67 mm) ve P.savastanoi pv. nerii (6.33-18.33 mm) ye karşı oldukça güçlü antibakteriyel etkinlik göstermiştir. Lamiaceae familyasına ait bitkilerin uçucu yağlarının genellikle Lauraceae ve Apiaceae familyalarına göre daha etkili olduğu bulunmuştur. O.syriacum, T.serpyllum ve T.spicata var. spicata, test edilen tüm bakteri türlerine karşı en yüksek antibakteriyel aktiviteleri gösteren umut verici uçucu yağlar olarak belirlenmiştir. Bu çalışmanın bulguları uçucu yağların gal oluşturan bakteriyel hastalık etmenlerine karşı antibakteriyel ajan olarak kullanılma potansiyeline sahip olduğunu göstermiştir.

Chemical Composition and Antibacterial Activity of Essential Oils Isolated from Medicinal Plants against Gall Forming Plant Pathogenic Bacterial Disease Agents

The aims of this study were to determine chemical composition ofessential oils from different plant species and their antibacterialactivities against gal-forming plant pathogenic bacterial diseaseagents Rhizobium radiobacter, Pseudomonas savastanoi pv.savastanoi and P.savastanoi pv. nerii. The chemical compositions ofessential oils were identified by GC/MS. The major constituents of theessential oils were carvacrol in Thymbra spicata var. spicata (66.88%)and Origanum syriacum (79.8%), thymol in Thymus serpyllum(41.03%), geranial in Thymus sipyleus (13.72%) and Melissa officinalis(30.4), 4-terpineol in Origanum majorana (31.67%), linalool inOcimum basilicum (30.23%), carvone in Mentha spicata (55.58%), 1,8cineole in Lavandula stoechas var. stoechas (35.5%), Laurus nobilis(35.5%) and Rosmarinus officinalis (18.47%), camphor in Salviaofficinalis (24.59%) and trans-anethole in Foeniculum vulgare (82.8%)essential oils, respectively. Based on inhibition zone diameter values,essential oils showed very strong antibacterial activities againstP.savastanoi pv. savastanoi (7.0-44.67 mm), followed by R.radiobacter(9.6-37.67 mm) and P.savastanoi pv. nerii (6.33-18.33 mm). Essentialoils of plants belong to Lamiaceae family were generally found to bemore efficient than those belong to Lauraceae and Apiaceae families.The essential oils of O.syriacum, T.serpyllum and T.spicata var.spicata were found to be the most promising essential oils displayingthe highest antibacterial activities against all tested bacterial species.The findings of the present study revealed that essential oils have apotential to be used as antibacterial agents against gall formingbacterial disease agents.

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