Diyabetik Ratlarda Zakkum (Nerium oleander L.) Çiçeği Etanolik Liyofilize Ekstresinin Eritrosit Frajilite, Hematolojik ve Antioksidan Etkilerinin Araştırılmas

Bu çalışma streptozotosin (50 mg kg-1) ile Tip 1 diyabet oluşturulanratlarda zakkum (N. oleander) çiçeğinden elde edilen etanolikliyofilize ekstresinin eritrosit frajilite, hematolojik parametreler, totalantioksidan kapasite (TAS), total oksidan kapasite (TOS) ve oksidatifstress indeksi (OSI) üzerine olan etkilerine odaklandı. Toksisite testisonrası, 49 rat 7 gruba ayrıldı. Kontrol, Nerium oleander (NO) (50 mgkg1), Diabetes mellitus (DM), DM+Gly-3 (3 mg kg1), DM+NO-25 (25mg kg1), DM+NO-75 (75 mg kg1) ve DM+NO-225 (225 mg kg1). 21günlük uygulamadan sonra elde edilen bulgulara göre, DM grubundaeritrosit hemolizi (%0.2-0.6 NaCI konsantrasyonlarda) kontrolgruplarına göre önemli artış gösterirken (P

Investigation of the Erythrocyte Fragility, Hematological and Antioxidant Effects of Oleander (Nerium oleander L.) Flower Ethanolic Lyophilized Extract in Diabetic Rats

The present study was focused on the erythrocyte fragility, hematological parameters, total antioxidant capacity (TAS), total oxidant capacity (TOS) and oxidative stress index (OSI) of flower ethanolic lyophilized extract obtained from N. oleander plant on Streptozotocin (50 mg kg-1)-induced Type 1 diabetes in rats. After toxicity test, 49 rats were divided into 7 groups. Control, Nerium oleander (NO) (50 mg kg-1), Diabetes mellitus (DM), DM+Gly-3 (3 mg kg-1), DM+NO-25 (25 mg kg-1), DM+NO-75 (75 mg kg-1) and DM+NO225 (225 mg kg-1). Based on the results, after 21 days of application, erythrocyte hemolysis of DM group was increased (at 0.2-0.6% NaCI concentrations) compared to Control groups whereas treatment groups significantly decreased (P

PDF

___

Abdou RH, Basha WA, Khalil WF 2019. Subacute Toxicity of Nerium oleander Ethanolic Extract in Mice. Toxicological research 35 (3): 233.
Al-Yahya MA, Al-Farhan AH, Adam SEI 2000. Preliminary toxicity study on the individual and combined effects of Citrullus colocynthis and Nerium oleander in rats. Fitoterapia 71(4): 385- 391.
American Diabetes Association. 2018. 2. Classification and diagnosis of diabetes: standards of medical care in diabetes-2018. Diabetes care 41 (Supplement 1): S13-S27.
Bandara V, Weinstein SA, White J, Eddleston M 2010. A review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetia peruviana (yellow oleander) poisoning. Toxicon 56: 273–281.
Bavunoğlu I, Balta M, Türkmen Z 2016. Oleander poisoning as an example of self-medication attempt. Balkan medical journal 33 (5): 559.
Baytop T, Baytop A, Mat A, Sun S 1989. Türkiyede zehirli bitkiler, bitki zehirlenmeleri ve tedavi yöntemleri. İstanbul Üniversitesi Yayınları, 3560, 975- 404-111-3.
Boswell BR, Dorweiler MA, Erbs NC, Chm B, Caplan JP 2013. A case of Nerium oleander toxicity: a thorny predicament. Psychosomatics (Washington, DC) 54 (4): 379-381.
Botelho AFM, Santos-Miranda A, Joca HC, Mattoso CRS, de Oliveira MS, Pierezan F, Cruz JS, SotoBlanco B, Melo MM 2017. Hydroalcoholic extract from Nerium oleander L. (Apocynaceae) elicits arrhythmogenic activity. Journal of ethnopharmacology 206: 170-177.
Dalar A, Dogan A, Bengu AS, Mukemre M, Celik I 2018. Screening in vivo antioxidant and haematological properties of sumac and acorn bioactive rich extracts. Industrial Crops and Products 124: 20-27.
Dey P, Saha MR, Chowdhuri SR, Sen A, Sarkar MP, Haldar B, Chaudhuri TK 2015. Assessment of antidiabetic activity of an ethnopharmacological plant Nerium oleander through alloxan induced diabetes in mice. Journal of ethnopharmacology 161: 128- 137.
Dogan A, Celik I, Kaya MS 2015. Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats. Journal of ethnopharmacology 176: 243-251.
Doğan A 2018. CCI4 İle oksidatif stres oluşturulan sıçanlarda Pholiota aurivella liyofilize ekstrenin bazı biyokimyasal ve hematolojik parametrelere etkisi. Mantar Dergisi 9 (1): 58-66.
Erel O 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 37(4): 277–285.
Erel O 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38(12): 1103–1111.
Ergun B 1992. Nerium oleander’in bazı suda çözünen bileşiklerinin in vitro biyolojik etkileri. Anadolu Üniversitesi Sağlık Bilimleri Enstitüsü Farmakoloji Anabilim Dalı, Doktora Tezi, 56 sy.
Farkhondeh T, Kianmehr M, Kazemi T, Samarghandian S, Khazdair MR 2020. Toxicity effects of Nerium oleander, basic and clinical evidence: A comprehensive review. Human & Experimental Toxicology 0960327120901571.
Hase GJ, Deshmukh KK, Murade VD, Pokharkar RD, Phatanagre ND, Hase DP, Dichayal S, Gosavi AB 2016. Phytopharmacology of Nerium oleander L. A review. International Journal of Phytopharmacology 7 (2): 0975-9328.
Inci A, Olmaz R, Sarı F, Coban M, Ellidag HY, Sarıkaya M 2016. Increased oxidative stress in diabetic nephropathy and its relationship with soluble Klotho levels. Hippokratia 20 (3): 198.
Kgosana KG 2019. The effects of extraction techniques and quantitative determination of oxalates in Nerium oleander and feeds. Onderstepoort Journal of Veterinary Research 86 (1): 1-9.
Kumar A, De T, Mishra A, Mishra AK 2013. Oleandrin: A cardiac glycosides with potent cytotoxicity. Pharmacognosy reviews 7 (14): 131.
Kumar D, Al Hassan M, Naranjo MA, Agrawal V, Boscaiu M, Vicente O 2017. Effects of salinity and drought on growth, ionic relations, compatible solutes and activation of antioxidant systems in oleander (Nerium oleander L.). Plos one 12 (9).
Kung CM, Tseng ZL, Wang HL 2009. Erythrocyte fragility increases with level of glycosylated hemoglobin in type 2 diabetic patients. Clinical hemorheology and microcirculation 43 (4): 345-351.
Miikue-Yobe TFB 2015. Effect of aqueous leaf extract of Heinsia crinata on haematological and some biochemical indices of toxicity in streptozotocin induced diabetic rats. International Journal for Innovative Research in Science & Technology 2: 116-126.
Mohadjerani M 2012. Antioxidant activity and total phenolic content of Nerium oleander L. grown in North of Iran. Iranian journal of pharmaceutical research: IJPR 11(4): 1121.
Pradeep SR, Srinivasan K 2018. Haemato-protective influence of dietary fenugreek (Trigonella foenumgraecum L.) seeds is potentiated by onion (Allium cepa L.) in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy 98: 372-381.
Rabbani SI, Devi K, Khanam S 2010. Protective role of glibenclamide against nicotinamide-streptozotocin induced nuclear damage in diabetic Wistar rats. Journal of pharmacology & pharmacotherapeutics 1(1): 18-23.
Sureka C, Ramesh T, Begum VH 2015. Attenuation of erythrocyte membrane oxidative stress by Sesbania grandiflora in streptozotocin-induced diabetic rats. Biochemistry and Cell Biology 93 (4): 385-395.
Thomas MC, MacIsaac RJ, Tsalamandris C, Power D, Jerums G 2003. Unrecognized anemia in patients with diabetes: a cross-sectional survey. Diabetes care 26 (4): 1164-1169.