Perkloretilen’in in vitro Sitotoksik Etkisinin Brine Shrimp Letalite Testi ile Araştırılması
Perkloroetilen (PERC), kuru temizleme endüstrisinde çözücü, yağgiderici ve temizleyici olarak yaygın bir şekilde kullanılmaktadır.Perkloroetilen, güçlü ve etkili bir temizleyici olup giysilerinburuşmasını ve renginin solmasını engeller. Kuru temizlemedükkanlarının en az üçte ikisi kuru temizleme işlemlerindeperkloroetilen’i kullanmaktadır. Uluslararası Kanser AraştırmalarıAjansı (IARC, 1995) Perkloroetileni insan karsinojeni olarak Grup2Aiçerisine almıştır ve kurutemizleme endüstrisinde çalışan insanlariçin kanserojen olabileceğini belirtmiştir. PERC’in kuru temizlemedeçalışan işçilerde cilt lezyonlarının yanı sıra, lenfosarkom, lösemi,kolon, akciğer ve ürogenital kanserlere neden olduğuna dairçalışmalar bulunmaktadır. Bu çalışmada saf Perkloroetilen’inArtemia salina larvaları üzerindeki potansiyel sitotoksik etkisi BrineShrimp Letalite Testi ile araştırılmıştır. Denemelerde negatif kontrololarak tuzlu su, çözücü kontrol olarak etanol ve pozitif kontrol olarakda Mitomisin-C (MMC) kullanılmıştır. Çalışmanın sonuçları, LC 50değerlerine göre, in vitro ortamda PERC’e 24 saat maruz bırakılanA.salina larvaları üzerindeki sitotoksik etkinin sadece 1000 ppm’likPERC uygulanması sonucunda ortaya çıktığını, diğer konsantrasyonaralıklarında (10 ve 100 ppm) ise sitotoksik aktivitesinin olmadığınıgöstermiştir.
Investigation of in vitro Cytotoxic Effects of Perchloroetylene by Using Brine Shrimp Lethality Assay
Perchlorethylene (PERC= tetrachlorethylene) is widely used in industry as a dry-cleaning solvent, degreaser, and cleaner. PCE is a useful solvent in the dry-cleaning industry because it is an effective cleaner and prevent shrinking and color fading of garments. At least two-thirds of dry cleaners use PCE as a solvent in their dry-cleaning operations. The International Agency for Research on Cancer (IARC) included PCE to the Group 2A probable human carcinogen, and considers it as possibly carcinogenic to the dry-cleaning industry workers. It has been showed that PERC causes human skin lesions as well as lymphosarcoma, leukemia, colon, lung and urogenital cancers in dry cleaning workers. In this study, the potential cytotoxic effect of pure Perchloroethylene on Artemia salina larvas was investigated by Brine Shrimp Letality Assay. In experiments saline was used as negative control, ethanol was used as solvent control and Mytomicin- C (MMC) was used as positive control. The results of this study (LC 50 values) showed that the cytotoxic effect on the A. salina larvae exposed to PERC for 24 hours in vitro was only due to the application of 1000 ppm of PERC, while the other concentrations (10 and 100 ppm) did not have cytotoxic activity.
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