CENK SONER BÖLÜKBAŞ, GÖKMEN ZAFER PEKMEZCİ, ALİ TÜMAY GÜRLER, MUSTAFA AÇICI, ŞİNASİ UMUR

Zoonotic trichuris trichiura infections in Non-Human primates at Samsun zoo, Turkey: First molecular characterization

Samsun Hayvanat Bahçesi’ndeki rehesus maymunu (Macaca mulatta) ve babun’dan (Papio hamadryas) Trichuris trichiura toplanmıştır. Moleküler karakterizasyon için Papio hamadryas’dan toplanan ergin parazitin DNA’sı izole edildi. Ribosomal DNA’nın ITS gen bölgesi çoğaltıldı ve NC5-NC2 primer çifti ile iki yönlü dizi analizi yapıldı. T. trichiura Türkiye izolatı (KC877992) ile diğer bölgelere ait Çin (AM992981, AM992985, AM992998), Çek Cumhuriyeti (JF690940, JF690941, JF690950), Hollanda (JF690948) ve Güney Afrika Cumhuriyeti (GQ301551, GQ301553) izolatlarının tüm ITS gen bölgesi arasındaki uzaklık indeksi %0.1 ile %1.2 arasında değişiklik gösterdi. Bu çalışma ile Türkiye’de T. trichiura’nın ITS bölgesinin ilk kez moleküler karakterizasyonu yapılmıştır.

Samsun Hayvanat Bahçesi’ndeki primatlarda zoonotik Trichuris trichiura Enfeksiyonu: İlk Moleküler Karakterizasyon

Trichuris trichiura were collected from rhesus macaque (Macaca mulatta) and Hamadryas baboon (Papio hamadryas) in Samsun Zoo, Turkey. DNA was isolated from individual worm in baboon for molecular characterization. The ITS region was amplified and sequencing in both directions using NC5-NC2 primers. Pairwise comparison between ITS region of the T. trichiura isolates from Turkey (KC877992) and other isolates China (AM992981, AM992985, AM992998), Czech Republic (JF690940, JF690941, JF690950), Netherlands (JF690948) and South Africa (GQ301551, GQ301553) showed differences ranging from 0.1% to 1.2%. With the present study, T. trichiura from Turkey were characterized for the first time by sequencing of the ITS.

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