The effect of vermiculite as litter material on some health and stress parameters in broilers
Bu çalışmada, farklı altlık materyallerinin ALT, AST; TP, ALB, GLU, CHOL, SOD, MDA, CAT, ayak taban yanığı, yürüme skoru, tonik immobilite ve tüylenme skoru gibi bazı sağlık ve stres parametreleri üzerine etkisini belirlemek amaçlanmıştır. Araştırmada 195 adet günlük civciv kullanıldı. Civcivler üç farklı altlık materyali (talaş, talaş-vermikülit karışımı ve vermikülit) kullanılan bölmelere yerleştirildi. Deneme 42 günlük yaşa kadar sürdürüldü. Denemenin sonunda ALT, AST, TP, ALB, GLU, CHOL, CAT ve tüylenme skoru bakımından altlık materyalleri arasında fark bulunamadı (P>0.05), ayak taban yanığı, yürüme skoru, tonik immobilite, SOD ve MDA değerleri bakımından ise altlık grupları arası fark önemlidir (P
Vermikülitin broilerlerde altlık olarak kullanımının bazı sağlık ve stres parametreleri üzerine etkisi
This study was conducted to determinine the effect of vermiculite as the litter material on some health and stress parameters such as alanin aminotransferaz (ALT), aspartate aminotransferase (AST), total protein (TP), albumin (ALB), glucose (GLU), cholesterol (CHOL), superoxide dismutases (SOD), malondialdehyde (MDA), catalase (CAT), footpad burn (FPB), gait score (GS), tonic immobility duration (TI) and feathering score (FS) in broilers. In present study, 195 day-old chickens were divided into three treatment groups placed on litter materials such as woodshavings (WS), mixture of woodshavings-vermiculite (WSV) and vermiculite (V), respectively. The experiment lasted for 42 days. At the end of the experiment, it was ascertained that the litter material did not have significant effect on ALT, AST, TP, ALB, GLU, CHOL, CAT and feathering score, but had significant effect on FPB, GS, TI, SOD and MDA values (P<0.01). FPB and GS were lower in the pens littered with V and WSV than in the pen littered with WS. The foot health was positively affectef by the use of V and WSV as litter material. TI of WS group was longer than those of V and WSV groups. SOD, MDA values from oxidative stress parameters were found lower (P<0.01) in the V and WSV groups than that of WS group. Consequently, vermiculite may be used as litter material in the rearing of broiler without adverse health problem.
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- 1. Laçin E, Çoban Ö, Aksu MI, Sabuncuoğlu N, Daş H: The effects of different breeding methods on fattening performance and parameters related to slaughter, carcass and some meat quality in broiler chickens. Kafkas Univ Vet Fak Derg, 19 (2): 283-289, 2013, DOI: 10.9775/ kvfd.2012.7678
- 2. Miller J: Alternative litter materials for poultry. Agfact A5.1.9, NSW Agriculture. Embury, Former, Livestock Officer (Poultry) Division of Animal Production. 2004. http://www.dpi.nsw.gov.au/__data/assets/pdf_file/0004/ 134446/Alternative-littermaterials-for-poultry.pdf, Accessed: 15.04.2013.
- 3. Andrews LD, Whiting TS, Stamps L: Performance and carcass quality of broilers grown on raised floorings and litter. Poultry Sci, 69, 1644-1651, 1990.
- 4. Sarica M, Cam MA: Potential of hazelnut husks as a broiler litter material. Brit Poultry Sci, 41 (5): 541-543, 2000.
- 5. Miles DM, Rowe DE, Cathcart TC: Litter ammonia generation: Moisture content and organic versus inorganic bedding materials. Poultry Sci, 90, 1162-1169, 2011.
- 6. Arunlertaree C, Soonngam L, Hutacharoen R: Vermiculite and hydrated sodıum calcium aluminosilicates as the agent of Aflatoxin B1 absorption for Black Tiger shrimp diets. Environ Nat Resour J, 5 (1): 50, 2007.
- 7. Toksoy F: Vermikülit: Mineraloji, jeolojik oluşum, endüstriyel kullanım ve Türkiye’deki durumu. 2. Endüstriyel Hammaddeler Sempozyumu, 16-17 Ekim, İzmir, Türkiye, pp.123-139, 1997.
- 8. Horosova K, Bujnakova D, Kmet V: Effect of oregano essential oil on chicken Lactobacilli and E. coli. Folia Microbiol, 51, 278-280, 2006.
- 9. Ratledge C, Wilkinson SG: An overview of microbial lipids. In, Ratledge C, Wilkinson SG (Eds): Microbial Lipids. 1, 3-22, Academic Press, London, 1988.
- 10. Knowles TG, Kestin SC, Haslam SM, Brown SN, Green LE, Butterworth A, Pope SJ, Pfeiffer D, Nicol CJ: Leg disorders in broiler chickens: prevalence, risk factors and prevention. Plos One, 3 (2): e1545. DOI:10.1371/ journal.pone.001545, 2008.
- 11. Roulston N: Lameness in Poultry: Evaluating Gait Scores, 2012. http:// poultrywelfarecentre.files.wordpress.com/2012/05/lameness-in-poultry- evaluating-gait-scores.pdf, Accessed: 15.04.2013.
- 12. Su G, Sørensen P, Kestin SC: Research Notes. A note on the effects of perches and litter substrate on leg weakness in broiler chickens. Poultry Sci, 79, 1259 -1263, 2000.
- 13. SCAHAW (Scientific Committee on Animal Health and Animal Welfare): The Welfare of Chickens Kept for Meat Production (Broilers). European Commission, Health and Consumer Protection Directorate- General, March, 2000.
- 14. Garcia RG, Almeida Paz ICL, Caldara FR, Nääs IA, Bueno LGF, Freitas LW, Graciano JD, Sim S: Litter materials and the incidence of carcass lesions in broilers chickens. Rev Bras Cienc Avic, 14 (1): 27-32, 2012.
- 15. Dozier WA 3rd, Thaxton JP, Branton SL, Morgan GW, Miles DM, Roush WB, Lott BD, Vizzier-Thaxton Y: Stocking density effects on growth performance and processing yields of heavy broilers. Poultry Sci, 84, 1332-1338, 2005.
- 16. Estevez I: Poultry welfare issues. Poultry Digest Online, 3(2):1- 12.2002. http://ansc.umd.edu/extension/poultry/documents/Poultry_ Welfare_Behavior/publications/Poultry%20Welfare%20Issues,%20 Poultry%20Digest%20Onine%20Volume%203%20Number%202.pdf, Accessed: 15.04.2013.
- 17. Jones RB: Tonic immobility reaction of the domestic fowl: A review. World Poult Sci J, 42, 82-96, 1986.
- 18. Edens FW: Empenamento em frangos: influência de aminoácidos e minerais na dieta. Anais da Conferência Apinco de Ciência e Tecnologia Avícolas, Campinas São Paulo. Brasil, pp.81-100, 2000.
- 19. Blumberg J: Use of biomarkers of oxidative stres in research studies. J Nutr, 134, 3188-3189, 2004.
- 20. Kestin S, Knowles T, Tinch A, Gregory N: Prevalence of leg weakness in broiler chickens and its relationship with genotype. Vet Rec, 131 (9): 190, 1992.
- 21. Jones RB, Faure JM: Sex and strain comparisons of tonic immobility. Behav Processes, 6 (1): 47-55, 1981.
- 22. Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500, 1988.
- 23. Yoshioka T, Kawada K, Shimada T, Mori M: Lipid peroxidation in maternal and cord blood and protective mechanism against activated- oxygen toxicity in the blood. Am J Obstet Gynecol, 135 (3): 372-376, 1979.
- 24. Goth L: A simple method for determenation of serum catalase activity and revision of serum catalase activity and revision of reference range. Clin Chim Acta, 196, 143-152, 1991.
- 25. Statistical Packages for the Social Sciences: SPSS for Windows Release 10.01. SPSS Inc., Chicago, USA, 1996.
- 26. Sorbara JOB, Rizzo MF, Laurentiz AC: Avaliação da polpa peletizada como material para cama de frangos de corte. Rev Bras Cienc Avic, 2 (3): 1-13, 2000.
- 27. Santos RL, Nunes VA, Baião NC: Pododermatite de contato em frangos de corte. Arq Bras Med Vet Zootec, 54 (6): 655-658, 2002.
- 28. Campo JL, Gil MG, Davila SG, Munoz I: Influence of perches and footpad dermatitis on tonic immobility and heterophil to lymphocyte ratio of chickens. Poultry Sci, 84,1004-1009, 2005.
- 29. Davis, JMG: In vivo assays to evaluate the pathogenic effects of minerals in rodents. In, George D, Guthrie Jr, Mossman BT (Eds): Healty Effects of Mineral Dusts. Rew Mineral, 28, 471-488, 1993.
- 30. Yalçınkaya İ, Güngör T, Başalan M, Çınar M, Saçaklı P: Broyler rasyonlarında organik selenyum ve vitamin E kullanımının performans, iç organ ağırlıkları ve kan parametreleri üzerine etkisi. Kafkas Univ Vet Fak Derg, 16 (1): 27-32, 2010.
- 31. Seven PT, Seven İ, Yılmaz S, Dalkılıç B: The effects of selenium and Vitamin C supplementation on lipid peroxidation in broilers reared cold environment (15°C) and diets of high energy. Fırat Üniv Sağ Bil Vet Derg, 23 (1): 15-19, 2009.
- 32. Uyanık F, Liman BC, Liman N: Danofloksasinin etlik piliçlerde bazı biyokimyasal parametreler ve karaciğer üzerine etkisi. Turk J Vet Anim Sci, 23 (4): 757-764, 1999.
- 33. Yalçınkaya İ: Broyler rasyonlarında enerji kaynağı olarak ayçiçek yağı yerine lesitin katkılı karma yağın kullanılma olanakları. Ankara Üniv Vet Fak Derg, 52, 63-68, 2005.
- 34. Arivuchelvan A, Murugesan S, Mekala P: Antioxidant properties of Ocimum sanctum in broilers treated with high doses of gentamicin. Ind J Drugs Dis, 1 (6): 2278-2958, 2012.