MURAT ŞEVİK

Molecular detection of peste des petits ruminants virus from different organs/tissues of naturally ınfected animals

Doğal enfekte koyun ve keçilerin farklı organ/dokularından PPR virusunun (PPRV) tespit oranının araştırılmasının amaçlandığı bu çalışmada, her biri farklı sürüye ait PPR şüpheli 24 hayvan, farklı organ/dokularda PPRV varlığı yönünden konvansiyonel reverz transkriptaz polimeraz zincir reaksiyonu (RT-PCR) ve real time RT-PCR yöntemleri ile incelenmiştir. PPRV spesifik antikor yanıtını belirlemek için virus nötralizasyon testi (VNT) kullanılmıştır. Real time RT-PCR ve RT-PCR yöntemleri ile test edilen 16 VNT pozitif hayvanın bütün akciğer, dalak, karaciğer ve lenf nodülü örnekleri pozitif olarak tespit edilirken, VNT negatif hayvanlardan (n=8) elde edilen bütün akciğer, dalak, karaciğer ve lenf nodülü örnekleri negatif olarak belirlenmiştir.

Doğal enfekte hayvanların farklı organ/dokularında koyun ve keçi vebası virusunun moleküler tespiti

PPR virus (PPRV) detection rate in different organs/tissues of naturally infected sheep and goats was investigated. In order to achieve this, 24 animals, each from different flocks, with PPR suspect were examined for the presence of PPRV nucleic acid in different organ/tissues by reverse transcriptase PCR (RT-PCR) and real time RT-PCR. Virus neutralizing test (VNT) was used for the determination of PPRV specific antibody response. Real time RT-PCR and RT-PCR were found positive in all of lung, spleen, liver and lymph node samples tested in each of 16 VNT-positive animals and were negative in all samples from VNT-negative animals (n=8).

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