Hakan Can YILDIRIM, ŞEYHO CEM YÜCETAŞ, Miktat KAYA, CEM ÖZİÇ, İNAN KAYA, B. Çağlar BİLGİN, Mehmet BALİOĞLU, MAHMUT KARAPEHLİVAN, Tezcan AKIN, Birkan TOPÇU

Investigation of the effects of alpha lipoic acid application on total antioxidant and oxidant status, paraoxonase, and total sialic acid levels in laminectomized rabbits

Bu çalışma, laminektomi yapılan tavşanlarda intramüsküler Alfa Lipoik Asit (ALA) uygulamasının total antioksidan kapasite (TAS), total oksidan kapasite (TOS), paraoksonaz (PON), ve total sialik asit (TSA) düzeylerini üzerine olan etkilerini araştırmak amacı ile yapıldı. Çalışmada, 3.5-4 kg ağırlığında 24 adet beyaz Yeni Zelanda erkek tavşan kullanıldı. Araştırma, herbiri 6 adet hayvan içeren 1 kontrol ve 3 deneme grubu olmak üzere 4 grup halinde yürütüldü. Kontrol grubuna hiçbir müdahale yapılmadı. Grup I’deki hayvanlara laminektomi yapılmaksızın 50 mg/ kg/gün İM ALA (Thioctacid 600 T, M EDa, Hamburg, Germany) enjekte edildi. Grup II’deki hayvanlara sadece laminektomi yapıldı. Grub III’e İM ALA+laminektomi uygulandı. Deneme toplamda 45 gün sürdürüldü. Denemenin 0., 15., 30.,ve 45. günlerinde vena auricularisten kan alındı. Elde edilen serum örneklerinde TAS, TOS, PON ve TSA düzeyleri belirlendi. Sonuç olarak, laminektomi yapılan tavşanlarda yangısal sürecin baskılanması ve iyileşmenin hızlandırılması için eksojen ALA kullanılmasının faydalı olabileceği kanısına varıldı.

Laminektomi yapılan tavşanlarda alfa-lipoik asit uygulamalarının total antioksidan kapasite, total oksidan kapasite, paraoksonaz ve total sialik asit düzeyleri üzerine etkilerinin araştırılması

The objective of this study is to investigate the effect of intramuscular administration of alpha-lipoic acid (ALA) on total antioxidant (TAS), total oxidant (TOS), paraoxonase (PON) and total sialic acid (TSA) levels in laminectomized rabbits. In the study, twenty four white New Zeeland rabbits weighing 3-4 kg were used. The investigation was carried out with three experiment and one control group consisting of six animals of each. No intervention has been done to the control group. Animals in the Group I received 50 mg/kg/day IM ALA (Thioctacid 600 T, M, EDA, Hamburg) without undergoing laminectomy. Group II underwent laminectomy; Group III underwent laminectomy and treated with IM ALA. The experiment lasted 45 days and blood samples were taken on 0, 15th, 30th, and 45th days from the animals. TAS, TOS, PON and TSA levels were measured in the serum. We conclude that ALA treatment would be beneficial in laminectomized rabbits in order to suppress inflammatory process and to expedite recovery.

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