KÜRŞAT ALTAY, NAZİR DUMANLI, Münir AKTAŞ, Sezai ÖZÜBEK

Survey of anaplasma ınfections in small ruminants from east part of Turkey

Bu çalışma Bingöl, Elazığ, Malatya ve Muş yöresindeki koyun ve keçilerde Anaplasma ovis ve Anaplasma phagocytophilum’un araştırılması amacıyla yapılmıştır. Rastgele seçilen 422 sağlıklı (291 koyun 131 keçi) hayvandan kan örneği alınmıştır. A. ovis için 60 kDA chaperonin gen (cpn60, hsp60 veya gro EL olarak da bilinir) ve Anaplasma phagocytophilum için 16S SSU rRNA genlerine spesifik primerler kullanılarak tür spesifik PCR yapılmıştır. PCR sonucunda toplam 301 (%71.32) hayvan A. ovis ve/veya A. phagocytophilum ile enfekte bulunmuştur. Örneklerin %67.06 (283/422)’sı A. ovis, %19.66 (83/422)’sı A. phagocytophilum ve %15.40 (65/422)’ı her iki tür yönünden pozitif olarak tespit edilmiştir. A. ovis ve A. phagocytophilum yönünden pozitif olan PCR ürünleri agaroz jelden purifiye edilerek sekanslanmıştır. Elde edilen DNA dizilimlerin daha önce bildirilen A. ovis ve A. phagocytophilum’a ait dizilimlerle aynı olduğu görülmüştür. Bu çalışma Doğu Anadolu Bölgesinde koyun ve keçilerde A. ovis ve A. phagocytophilum’un moleküler yöntemlerle teşhisi üzerine yapılan ilk araştırmadır. Türlerin genotipleri ve vektörlerinin belirlenmesine yönelik çalışmaların gerektiği düşünülmektedir.

Doğu Anadolu bölgesinde koyun ve keçilerde anaplasma enfeksiyonlarının araştırılması

This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants, species-specific PCRs were set up using 60 kDA chaperonin gene (cpn60, also known as hsp60 or groEL) and 16S SSU rRNA gene primer sets, respectively. A total of 301 (71.32%) animals were found infected with A. ovis and/or A. phagocytophilum. The percentages of positive animals for A. ovis and A. phagocytophilum were 67.06% (283/422) and 19.66% (83/422), respectively. The rate of concurrent infections was 15.40% (65/422). Four PCR products from positive samples were purified from agarose gel and sequenced. These sequences were identical to the reported nucleotide sequences of A. ovis and A. phagocytophilum. This is the first molecular based study on the detection of A. phagocytophylum and A. ovis in small ruminants from East Anatolia Region. Further studies are needed on the determination of the genotypes and vectors of the species.

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1. SC, Rikihisa Y, Rurangirwa R: Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: Unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol, 51, 2145-65, 2001.
2. Estrada-Pena A, Jongejan F: Ticks feeding on humans: A review of records on human-biting Ixodoidea with special reference to pathogen transmission. Exp Appl Acarol, 23, 685-715, 1999.
3. Friedhoff KT: Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp. Parassitologia, 39, 99-109, 1997.
4. Kocan KM, de la Fuente J, Blouin EF, Garcia-Garcia JC: Anaplasma marginale (Rickettsiales: Anaplasmataceae): Recent advances in defining host-pathogen adaptations of a tick-borne rickettsia. Parasitology, 129, 285-300, 2004.
5. de la Fuente J, Atkinson MW, Naranjo V, Fernández de Mera IG, Mangold AJ, Keating KA, Kocan KM: Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet Microbiol, 119, 375-381, 2007.
6. Tibbitts T, Goff WL, Foreyt W, Stiller D: Susceptibility of two rocky mountain bighorn sheep to experimental infection with Anaplasma ovis. J Wildlife Dis, 28, 125-129, 1992.
7. Altay K, Aktas M, Dumanli N, Aydin MF: Evaluation of PCR and comparison with RLB for detection and differentiation of Theileria and Babesia species of small ruminants. Parasitol Res, 103, 319-323, 2008.
8. Altay K, Dumanli N, Aktas M: A study on ovine tick-borne haemo- protozoan parasites (Theileria and Babesia) in the East Black Sea region of Turkey. Parasitol Res, 111, 149-153, 2012.
9. Özcan HC: Ankara ve civarında evcil hayvanlarda görülen Piroplasmose vakaları ve tedavileri üzerinde araştırmalar. Ankara Üniv Vet Fak Yay, Ankara,1961.
10. Sayın F, Dinçer Ş, Karaer Z, Çakmak A, Yukarı BA, Eren H, Değer S, Nalbantoğlu S: Status of the tick-borne diseases in sheep and goats in Turkey. Parasitologia, 39 (2): 153-156, 1997.
11. Aktas M, Altay K, Dumanli N, Kalkan A: Molecular detection and identification of Ehrlichia and Anaplasma species in ixodid ticks. Parasitol Res, 104, 1243-1248, 2009.
12. Aktas M, Vatansever Z, Altay K, Aydin MF, Dumanli N: Molecular evidence for Anaplasma phagocytophilum in Ixodes ricinus from Turkey. T Roy Soc Trop Med H, 104, 10-15, 2010.
13. Aktas M, Altay K, Dumanli N: Molecular detection and identification of Anaplasma and Ehrlichia species in cattle from Turkey. Ticks and Tick- borne Dis, 2, 62-66, 2011.
14. Gokce HI, Genc O, Akca A, Vatansever Z, Unver A, Erdogan HM: Molecular and serological evidence of Anaplasma phagocytophilum infection of farm animals in the Black Sea Region of Turkey. Acta Vet Hung, 56, 281-292, 2008.
15. Clausen PH, Wiemann A, Patzelt R, Kalkaire D, Potzsch C, Peregrine A, Mehlitz D: Use of a PCR assay for the specific and sensitive detection of Trypanosoma spp. in naturally infected dairy cattle in peri- urban Kampala, Uganda. Ann NY Acad Sci, 29, 21-31, 1999.
16. Kawahara M, Rikihisa Y, Lin Q, Isogai E, Tahara K, Itagaki A, Hiramitsu Y, Tajima T: Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp. in wild deer and ticks on two major islands in Japan. Appl Environ Microbiol, 72, 1102-1109, 2006.
17. Haigh JC, Gerwing V, Erdenebaatar J, Hill JE: A novel clinical syndrome and detection of Anaplasma ovis in Mongolian reindeer (Rangifer tarandus). J Wildlife Dis, 44, 569-577, 2008.
18. Santos AS, Santos-Silva MM, Almeida VC, Bacellar F, Dumler JS: Detection of Anaplasma phagocytophilum DNA in Ixodes ticks (Acari: Ixodidae) from Madeira Island and Setubal District, mainland Portugal. Emerg Infect Dis, 10, 1643-1648, 2004.
19. Aydin L, Bakirci S: Geographical distribution of ticks in Turkey. Parasitol Res, 101, 163-166, 2007.
20. Tonbak S, Aktas M, Altay K, Azkur AK, Kalkan A, Bolat Y, Dumanli N, Ozdarendeli A: Crimean-Congo hemorrhagic fever virus: Genetic analysis and tick survey in Turkey. J Clin Microbiol, 44 (11): 4120-4124, 2006.