Muhammet Lütfi SELÇUK, Fatma ÇOLAKOĞLU, Saadettin TIPIRDAMAZ

Yeni Zellanda Tavşanlarında Böbreğin Stereolojik ve Histomorfometrik Değerlendirilmesi

Çalışmanın amacı Yeni Zelanda tavşanlarında böbrek hacim ve hacim oranlarını stereolojik yöntemlerle belirlemek, tubulus proximalis, tubulus distalis, toplayıcık borucuk, Henle kulpu ve glomerulus sayılarının histomorfolojik özelliklerini ortaya koymak, sağ ve sol böbreklerin fonksiyonel alt bileşenleri arasındaki olası farkları ve cinsiyet farkının bunlara etkisini araştırmaktır. Çalışma 9 erkek ve 9 dişi sağlıklı Yeni Zelanda tavşanı böbreği üzerinde gerçekleştirildi. Böbrekler tartıldıktan sonra, çapları ve uzunlukları dijital kumpas yardımıyla ölçüldü. Sol ve sağ böbreği oluşturan alt bileşenlerinin toplam böbrek hacmi ve hacim oranları Cavalieri metodu kullanılarak hesaplandı. Örneklenen böbreklerden histolojik kesitler alındı ve birim alandaki böbrek yapıları sayıldı. Böbreği oluşturan bileşenlerin değerleri oransal olarak ifade edildikten sonra, cinsiyetler arasındaki farklılıkları ortaya çıkarmak için istatistiksel analiz gerçekleştirildi. Böbrek yoğunlukları arasında istatistiksel bir fark tespit edilemedi. Dişi ve erkek tavşanlarda sağ dorsoventral ve mediolateral çapların sol taraftan daha büyük olduğu tespit edildi (P0.05). Dişi tavşanlarda sol toplayıcı borucuk sayısının erkek tavşanlardan daha yüksek olduğu ve istatistiksel olarak anlamlı olduğu tespit edildi (P

Stereological and Histomorphological Assessment of New Zealand Rabbit Kidneys

The objectives of this study were to determine renal volume, volume ratios in New Zealand rabbits by stereological methods, reveal thehistomorphological properties of tubulus proximalis, tubulus distalis, collecting tubule, Henle’s loop and number of glomerulus. Besides, it is toinvestigate the possible diffrences between the functional subcomponents of the right and left kidneys and the effcts of gender discriminationon them. The study was carried out on 9 males and 9 females healthy New Zealand rabbits’ kidneys. After weighing kidneys, diameters and lengthswere measured with a digital caliper. Total kidney volume and volume fractions of subcomponents of left and right kidneys were estimated byCavalieri’s method. The histological section was taken from the sampled kidneys and kidney structures in the unit area were counted. After allvalues of each component were expressed as ratios with in kidney, they were analyzed statistically to reveal diffrences between sexes. There wasno statistical diffrence between the renal densities. The right dorsoventral and mediolateral diameters of the females and males were found tobe greater than the left (P0.05). It was determined that the number of left collecting tubules in female rabbits was higher than males and it was statistically significant(P

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