Ghulam ABBAS, Maryam JAVED, Muhammad Muddassir ALI, Muhammad Sajid Aqeel AHMAD, Masroor Ellahi BABAR, Muhammad Sajid TAHIR, Sadia TABASSUM, Wasim SHEHZAD

Mitochondrial Cytochrome-b, Cytochrome-c and d-loop Region Based Phylogenetic and Diversity Analysis in Blackbuck (Antilope cervicapra)

The present study was designed to find diversity analysis of Antilope cervicapra family in Pakistan. Fecal samples of Antilope cervicapra were collected from their diffrent habitats of Pakistan. Fecal DNA was extracted and Polymerase Chain Reaction (PCR) was performed. Sequencing was performed by Big DyeTM Terminator method. Diversity and phylogenetic analysis was performed by diffrent Bioinformatics tools. Less genetic variability was observed within Antilope cervicapra population through Multi-Dimensional Scaling (MDS). However, significant genetic variation was observed among other species and Antilope cervicapra. Phylogenetic analysis revealed distinct clade of this specie with respect to other species of deer. This is the first report from Pakistan that could help for designing effctive strategy in future conservation practices of deer species

Kara Antilop’ta (Antilope cervicapra) Mitokondriyal Sitokrom-b, Sitokrom-c ve d-loop Bölgelerine Dayalı Filogenetik ve Çeşitlilik Analizi

Bu çalışma Pakistan’daki Antilope cervicapra ailesinin çeşitlilik analizini belirlemek için tasarlandı. Antilope cervicapra dışkı örnekleri Pakistan’ın farklı habitatlarından toplandı. Fekal DNA ekstrakte edildi ve Polimeraz Zincir Reaksiyonu (PCR) yapıldı. Dizileme işlemi Big DyeTM Terminator metodu ile yapıldı. Çeşitlilik ve filogenetik analizlerde farklı biyoenformatik araçlar kullanıldı. Antilope cervicapra popülasyonunda Çok Boyutlu Ölçeklendirme (MDS) değerlendirmesinde düşük düzeyde genetik değişkenlik gözlendi. Bununla birlikte, diğer türler ve Antilope cervicapra arasında önemli genetik varyasyon belirlendi. Filogenetik analiz, bu türün diğer geyik türlerine göre belirgin bir tür olduğunu ortaya koydu. Bu araştırma, Pakistan’dan geyik türlerinin gelecekteki koruma uygulamalarında etkili bir strateji tasarlanmasına yardımcı olabilecek ilk rapordur.

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