MEHMET ELMALI, Hilmi YAMAN

Thermophilic campylobacter SPP. on frozen poultry carcasses

Kars bölgesinde Campylobacter türlerinin halk sağlığı açısından oluşturduğu riski belirlemek amacıyla marketlerden toplanan toplam 50 donmuş tavuk örneği termofilik Campylobacter türleri, Campylobacter jejuni (C.jejuni), Campylobacter coli (C. coli) ve Campylobacter lari (C. lari) yönünden analiz edildi. Campylobacter'lenn izolasyon ve identifikasyonu Gıda ve İlaç Dairesinin (PDA) önerdiği metoda göre yapılmıştır. 50 donmuş tavuk karkasının % 58.0'inden (29 örnek) termofilik Campylobacter spp. izole edildi. Bu izolatlarm 21'i (% 42.0) C.jejuni, 7'si (% 14.0) C. coli ve l'i C. lari olarak identifiye edildi. Bu yörede satışa sunulan kanatlı karkaslarının insan sağlığı açısından bir risk taşıdığı sonucuna varılmıştır.

Anahtar Kelimeler:

mikrobiyal bulaşıklık, tavuk, tavuk eti, Kars, halk sağlığı, termofilik bakteriler, Campylobacter, karkas, dondurulmuş gıdalar, kümes hayvanları

Donmuş piliç karkaslarında termofilik campylobacter türleri

A total of 50 frozen poultry samples were collected from the markets in Kars district and analyzed for the presence of thermophilic Campylobacter spp., Campylobacter jejuni (C.jejuni), Campylobacter coli (C. coli) and Campylobacter lari (C. lari). Campylobacter spp. were isolated and identified according to the method of Food and Drug Administration (FDA). Thermophilic Campylobacter spp. were isolated at the level of (29 samples) 58.0 % from 50 frozen chicken carcasses. 21 (42.0 %) of these isolates were identified as C.jejuni, 7 (14.0 %) isolates were C. coli and 1 (2.0 %) isolate was identified as C. lari. It is concluded that poultry carcasses consumed in their area pose a risk for human health.

Keywords:

microbial contamination, fowls, chicken meat, Kars, public health, thermophilic bacteria, Campylobacter, carcasses, frozen foods, poultry,

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1.Griffiths PL, Park RWA: Campylobacters associated with human diarrhoeal disease. JAppl Bacteriol, 69: 281-301, 1990.
2.Hascelik G, Akyon Y, Hayran M, Yurdakok K, Berkman E: Campylobacter as a cause of acute enteritis in Turkey. Therapy Infect Dis, 6: 288-292, 1991.
3.Anonymous: Campylobacter spp. laboratory reports. Public Health Laboratory Service. Arriving: http.// www.phls.uk. Date of arriving: 19.01.2001.
4.Alterkuse SF, Stern NJ, Fields PI, Swerdlow DL: Campylobacter jejuni-An emerging foodborne pathogen. Emerging Infect Dis, 5 (1): 1-12,1999.
5.Skirrow MB: Campylobacter. Lancet, 336: 921,1990.
6.Tauxe RV, Hargrett NB, Patton CM: Campylobacter isolates in the United States, 1982-1986. Arriving: http://www. usda.gov. Date of arriving: 19.01.2001.
7.Kotula A, Stern N: The importance of Campylobacter jejuni to the meat industry. J Anim Sci, 58 (6): 1561-1566, 1984.
8.Bolton FJ, Dawkins HC, Hutchinson D: Biotypes and serotypes of thermophilic campylobacters isolated from cattle, sheep, and pig offal and other red meats. J Hyg Camb, 95: 1-6, 1985.
9.Skirrow MB: Diseases due to Campylobacter, Helicobacter andreleatedbacteria. JCompPathol, 111: 113-149,1994.
10.Elmali M: Isolation and Identification Thermophilic 19.Campylobacter Species in the Bacteriological Meat Inspection Samples of Cattle. PhD. Theises, Institute of Health Science, University of Ankara, Ankara, 2001.
11.Arda M, Minbay A, Aydın N, Akay O, Izgur M, Leloglu N, Kahraman M, İlgaz A, Diker KS: Özel Mikrobiyoloji. 4th ed, Ankara, 1997.
12.Bolton FJ, Coates D: A study of the oxygen and carbon dioxide requirements of thermophilic campylobacters. J Clin Pathol, 36: 829,1983.
13.Mishu B, Blaser MJU: Role of infection due to Campylobacter jejuni in the initation of Guillain-Barre syndrome. J Infect Dis, 17(1): 104-108, 1993. .
14.Nishimura M, Nukina M, Kuroki S, Obayashi H, Ohta M, Ma J, Saida T, Uchiyama T: Characterization of Campylobacter jejuni isolates from patients with Guillain- Barre syndrome. J Neurol Sci, 153 (1): 91 -99, 1997.
15.Sillero M, Almirall J: Campylobacter jejuni and hemolytic uremic syndrome. J Hyg Comb, 82: 363-364, 1999.
16.Kita E, Oku D, Hamuro A, Nishikawa F, Emoto M, Yagyu Y, Katsui N, Kashiba S: Hepatotoxic activity of Campylobacter jejuni. J Med Microbiol, 33 : 171-182, 1990.
17.Izat AL, Gardner FA, Denton, JH, Golan FA: Incidence and level of Campylobacter jejuni in broiler processing. Poultry Sci, 67: 1568-1572,1988.
18.Boer DE, Hahne M: Cross-contamination with Campylobacter jejuni and Salmonella spp. from raw chicken products during food preparation. J Food Prot, 53 (12): 1067- 1068,1990.
19.Atabay HI, Corry JEL: The prevalence of campylobacters and arcobacters in broiler chickens, J Appl Microbiol, 83: 619- 626,1997.
20.Berrang ME, Dickens JA: Presence and level of Campylobacter spp. on broiler carcasses throughout the processing plant. Appl Poultry Sci, 9: 43f47, 2000.
21.Kwiatek K, Wojton B, Stern N: Prevalence and distribution of Campylobacter spp. on poultry and selected red meat carcasses in Poland. / Food Prot, 53 (2): 127-130,1990.
22.Kramer JM, Frost JA, Bolton FJ, Wareing DR: Campylobacter contamination of raw meat poultry at retail sale:identification of multiple types and comparasion with isolates from human infection. J Food Prot, 63 (12): 1654-9, 2000.
23.Zhao C, Ge B, Villena JD, Sudler R, Yen E, Zhao S, White DG, Wagner D, Meng J: Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from Greater Washington. D.C. area. Appl Environ Microbiol, 67(12): 5431-5436,2001.
24.Elmalı M: Presence of C. jejuni in carcass and ground meat marketed in Bitlis. Istanbul Üniv Vet Fak Derg, 30(2): 2004 (in Press).
25.Steinhauserova I, Fojtivola K: Serotyping and identification of Campylobacter jejuni and Campylobacter coli strains of human and animal origin using the PCR method. Acta Vet Brno, 68: 149-154, 1999. .
26.Corry JE, James C, O'neil D, Yaman H, Kendall A, Howell M: Physical methods, reaidly adapted to existing commercial processing plants, for reducing numbers of campylobacters, on raw poultry. (Abst.), Int J Medical Microbiol, (publishing). 2003.
27.Anonymous: Campylobacter jejuni. F.D.A., Arriving: http:// www.vm.cfsan.fda.gov., 2000; Date of arriving: 12.02.2000.
28.Siimbüloğlu K, Sümbüloğlu V: Biyoistatistik, 4th ed. Özdemir Yayın. Ankara. 2000.
29.Kazwala RR, Collins DJ, Hannan RA, Crinion PAR, Mahony HO: Factors responsible for the introduction and spread of Campylobacter jejuni infection in commercial poultry production. VetRec, 126: 305-306, 1990.
30.Nielsen EM, Nielsen N: Serotypes and typability of Campylobacter jejuni and Campylobacter coli isolated from poultry products. Int J Food Microbiol, 46 (3): 199-205,1999.
31 Özer D, Ergün O: Investigation on the presence of Campylobacter jejuni in .the various poultry meat and product marketing in Istanbul. Istanbul Üniv Vet Fak Derg, 25 (1): 81- 88, 1999.
32.Stern NJ, Green SS, Thaker N, Krout DJ, Chiy J: Recovery of Campylobacter jejuni from fresh and frozen meat and poultry collected at slaughter. J Food Prot, 47 (5): 372-374, 1984.
33.Atanassova V, Ring C: Prevalence of Campylobacter spp. In poultry and poultry meat in Germany. Int J Food Microbiol, 51 (2-3): 187-90,1999.
34.Genigeorgis C, Hassuneh M, Collins P: Campylobacter jejuni infection on poultry farms and its effect on poultry meat contamination during slaughtering. J Food Prot, 49 (11): 895- 903, 1986.
35.Fronco D: Campylobacter species: Consideration for controlling a foodborne pathogen., J Food Prot, 51 (2): 145- 153,1988.
36.Berrang ME, Buhr RJ, Cason JA: Campylobacter recovery from external and internal organs of commercial broiler carcass prior to sacalding. Poultry Sci, 79: 286-290, 2000.
37.Stern NJ, Robach MC: Enumeration of Campylobacter spp. in broiler feces and in corresponding processed carcasses. / Food Prot, 66 (9): 1557-63, 2003.
38.Oosterom J, Notermans S, Karman H, Negels GB: Origin and prevalence of Campylobacter jejuni in poultry processing., / Food Prot, 46 (4): 339-344, 1983.
39.Allerberger F, Al-Jazrawi N, KreidI P, Dierich MP, Feierl G, Hein I, Wagner M: Barbecued chicken causing a multi- state outbreak of Campylobacter jejuni enteritis. Infect, 31 (1): 19-23,2003.
40.Solow BT, Cloak OM, Fratamico PM: Effect of temperature on viability of Campylobacter jejuni and Campylobacter coli on raw chicken or pork skin. J Food Prot, 66 (11): 2023-31, 2003.
41.Blankenship LC, Craven SE: Campylobacter jejuni survival in chicken meat as a function temperature. Applied and Environ Microbiol, 44: 88-92,1982.
42.Tarjan V: Investigation into the radiosensitivity of Campylobacter fetus subsp. jejuni in ground chicken meat. Int J Food Microbiol, 1: 321-326,1985.
43.Bostan K, Aksu H, Özgen O, Uğur M: Effects of refregerated and frozen storage on the survival of Campylobacter jejuni in ground and cubed beef. Istanbul Üniv Vet Fak Derg, 27 (2): 623-630, 2001.
44.Beuchat LR: Efficacy of some methods and media for detecting and enumerating Campylobacter jejuni in frozen chicken meat. J Appl Bacteriol, 62: 217-221,1987.
45.Lee A, Smith SC, Coloe PJ: Survival and growth of Campylobacter jejuni after artificial inoculation onto chicken skin as a function of temperature and packaging conditions. J Food Prot, 61: 1609-1641,1998.
46 Baker RC, Paredes MDC, Qureshi RQ: Prevalence of Campylobacter jejuni in eggs poultry meat in New York State. Poultry Sci, 66: 1766-1770,1987.