The role of Enterococcal virulence factors on experimental amyloid arthropathy in chickens

Bu araştırmada, enterokokal virulens faktörlerinin tavukların amiloid artropatisinin oluşumu üzerindeki etkilerinin araştırılması amaçlandı. Çalışmada jelatinaz, agregasyon maddesi ve sitolizin yönünden çeşitlilik gösteren E. faecalis suşları 2 haftalık tavuklara intraartiküler ve 5 haftalık tavuklara intravenöz yollar ile inokule edildi. İntraartiküler deney gruplarında jelatinaz pozitif suşlar inokule edilen gruplarda %65.2, agregasyon maddesi pozitif suşlar verilen gruplarda %30.4 ve sitolizin pozitif suşlar verilen gruplarda %22.2 oranında amiloid artropati saptandı. İntravenöz deney gruplarında jelatinaz pozitif suşlar inokule edilen gruplarda %75, agregasyon maddesi pozitif suşlar verilen gruplarda %50 ve sitolizin pozitif suşlar verilen gruplarda %26.6 oranında bilateral amiloid artropati saptandı. Her iki deney grubunda da amiloid artropatiye neden olan suşlarda ortak olan tek virulens faktörünün jelatinaz olduğu belirlenirken, jelatinaz taşımayan suşların hiçbirisi amiloid artropatiye neden olmadı. Sonuç olarak, enterokokal jelatinazın tavuklarda deneysel amiloid artropati oluşumunda rol oynadığı ve jelatinaz pozitif E. faecalis suşlarının tavuklarda artritis, topallık ve büyüme geriliği ile karakterize amiloid artropatiye neden olabileceği kanısına varıldı.

Anahtar Kelimeler:

tavuk, Enterococcus faecalis, virülans, kümes hayvanları, topallık, eklemler (hayvan), eklem hastalıkları, büyüme geriliği, deneysel enfeksiyon, eklem yangısı

Tavukların deneysel amiloid artropatisinde enterokokal virulens faktörlerinin rolü

In this study, it was aimed to determine the role of enterococcal virulence factors on the development of amyloid arthropathy in chickens. Enterococcus faecalis strains phenotypically variable in gelatinase, aggregation substance and cytolisine traits were inoculated intraarticularly to chickens of two weeks and intravenously to chickens of five weeks. In intraarticular inoculation groups, amyloid arthropathy occurred in 65.2% of gelatinase positive strains, 30.4% of aggregation substance positive strains and 22.2% of cytolisine positive strains. In intravenous inoculation groups, bilateral amyloid arthropathy occurred in 75% of gelatinase positive strains, 50% of aggregation substance positive strains and 26.6% of cytolisine positive strains. Gelatinase was the only virulence factor common in all E. faecalis strains causing amyloid arthropathy, and none of the gelatinase negative strains caused the amyloid arthropathy in any of the chickens in both groups. In conclusion; it was suggested that enterococcal gelatinase plays a role in the development of experimental amyloid arthropathy and the gelatinase positive E. faecalis strains may cause amyloid arthropathy which is characterized by arthritis, lameness and growth retardation.

Keywords:

fowls, Enterococcus faecalis, virulence, poultry, lameness, joints (animal), joint diseases, growth retardation, experimental infections, arthritis,

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